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American Journal of Pathology, Vol 138, 1503-1509, Copyright © 1991 by American Society for Investigative Pathology

REGULAR ARTICLES

Quantitation of T-cell DNA in cutaneous lymphoid infiltrates

GS Wood, A Bourguin, CF Crooks and J Sklar
Department of Dermatology, Case Western Reserve, University School of Medicine, Cleveland, Ohio.

A method is described that allows the quantitation of T-cell DNA within a tissue biopsy specimen. This method is based on the densitometric evaluation of the multiband pattern of TCR-gamma gene rearrangements detectable in Southern blot analyses of polyclonal T-cell DNA digested with HindIII. These rearrangements can be detected down to a limit of approximately 5% T-cell DNA. The information derived from this type of analysis can be used to roughly assess the total T-cell content of lesional tissue specimens expressed as a percentage of total tissue DNA, and can be correlated with the pattern of T-cell infiltration visualized by immunohistology. This assessment can be used also to determine the threshold, expressed as a percentage of total lesional T- cell DNA instead of total tissue DNA, required for the detection of a monoclonal T-cell population by Southern blot analysis. These quantitative relationships may prove useful for studying cutaneous T- cell lymphoma and associated precursor conditions.


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Copyright © 1991 by the American Society for Investigative Pathology.