This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Legrand, A. Articles by Nachtigal, M. Search for Related Content PubMed PubMed Citation Articles by Legrand, A. Articles by Nachtigal, M.

American Journal of Pathology, Vol 139, 629-640, Copyright © 1991 by American Society for Investigative Pathology

REGULAR ARTICLES

Characterization of human vascular smooth muscle cells transformed by the early genetic region of SV40 virus

A Legrand, P Greenspan, ML Nagpal, SA Nachtigal and M Nachtigal
Department of Pathology, University of South Carolina School of Medicine, Columbia 29208.

Human arterial smooth muscle cells transfected with the plasmid pSV3- neo, which contains the SV40 virus early region and the neor gene, developed colonies of morphologically transformed cells. Five cell strains were initiated from these colonies and could be subcultivated for up to 9 months before entering a stage of crisis that ended their life span. Deoxyribonucleic acid (DNA) molecules containing viral sequences were found free and integrated in the transformed cells. The intranuclear SV40 large T antigen and the p53 cellular protein were expressed in the transformed cells. Most of the transformed cells were spindle shaped but some were large and multinucleated. The modal chromosome numbers were in the triploid range, and aberrations, particularly dicentrics, were common. The transcripts for smooth muscle actins were significantly reduced and there were less alpha-actin filaments detected by immunofluorescence. Cytochemical staining disclosed a large accumulation of lipid droplets in the transformed cells incubated with rabbit hypercholesterolemic beta-very-low-density lipoprotein. Chemical analysis showed that cholesteryl esters were significantly elevated in these cells. Phenotypic changes induced in human vascular smooth muscle cells by SV40 early genes are similar to those found in smooth muscle cells from atherosclerotic lesions and may indicate common pathogenetic mechanisms.

This article has been cited by other articles:

				J.-K. Hsieh, D. Kletsas, G. Clunn, A. D. Hughes, M. Schachter, and C. Demoliou-Mason p53, p21WAF1/CIP1, and MDM2 Involvement in the Proliferation and Apoptosis in an In Vitro Model of Conditionally Immortalized Human Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., April 1, 2000; 20(4): 973 - 981. [Abstract] [Full Text] [PDF]

				J.-K. Hsieh, D. Kletsas, G. Clunn, A. D. Hughes, M. Schachter, and C. Demoliou-Mason p53, p21WAF1/CIP1, and MDM2 Involvement in Proliferation and Apoptosis in an In Vitro Model of Conditionally Immortalized Human Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., March 1, 2000; 20(3): 636 - 644. [Abstract] [Full Text] [PDF]

				K. A. Hajjar, C. A. Guevara, E. Lev, K. Dowling, and J. Chacko Interaction of the Fibrinolytic Receptor, Annexin II, with the Endothelial Cell Surface. ESSENTIAL ROLE OF ENDONEXIN REPEAT 2 J. Biol. Chem., August 30, 1996; 271(35): 21652 - 21659. [Abstract] [Full Text] [PDF]

				L Jahn, J Sadoshima, A Greene, C Parker, K. Morgan, and S Izumo Conditional differentiation of heart- and smooth muscle-derived cells transformed by a temperature-sensitive mutant of SV40 T antigen J. Cell Sci., January 2, 1996; 109(2): 397 - 407. [Abstract] [PDF]

				M. Guittaut, S. Charpentier, T. Normand, M. Dubois, J. Raimond, and A. Legrand Identification of an Internal Gene to the Human Galectin-3 Gene with Two Different Overlapping Reading Frames That Do Not Encode Galectin-3 J. Biol. Chem., January 19, 2001; 276(4): 2652 - 2657. [Abstract] [Full Text] [PDF]