This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arbustini, E. Articles by Campana, C. Search for Related Content PubMed PubMed Citation Articles by Arbustini, E. Articles by Campana, C.

American Journal of Pathology, Vol 139, 709-715, Copyright © 1991 by American Society for Investigative Pathology

REGULAR ARTICLES

Expression of tumor necrosis factor in human acute cardiac rejection. An immunohistochemical and immunoblotting study

E Arbustini, M Grasso, M Diegoli, M Bramerio, AS Foglieni, M Albertario, L Martinelli, A Gavazzi, C Goggi and C Campana
Department of Pathology, Universita di Pavia, Italy.

The authors performed an immunohistochemical study on expression of tumor necrosis factor alpha (TNF alpha) in endomyocardial biopsies from human cardiac allografts. TNF alpha immunoreactivity was found in 45% biopsies with mild acute rejection, in 83% biopsies with focal moderate rejection, in 80% biopsies with diffuse moderate rejection. Biopsies with absent rejection did not show immunoreactive cells. In mild rejection, positive cells were few and scanty monocytes and macrophages (MAC-387 and LN5 positive cells) and T lymphocytes (UCHL-1/CD45 RO positive cells) (up to 20% of all infiltrating cells). Expression of major histocompatibility complex (MHC) class II antigens on infiltrating and endothelial cells occurred earlier and independent of TNF alpha reactivity. Number of immunoreactive cells increased in moderate rejection (up to 50%). Immunoreactivity was also present in nonpigmented macrophages in part of the biopsies with resolving rejection (45%). The authors conclude that TNF alpha is expressed in acute cardiac rejection by immunologically activated inflammatory cells. Immunoreactive cells increase in number with increasing severity of the reaction.

This article has been cited by other articles:

				M. Bellahcene, S. Jacquet, X. B. Cao, M. Tanno, R. S. Haworth, J. Layland, A. M. Kabir, M. Gaestel, R. J. Davis, R. A. Flavell, et al. Activation of p38 Mitogen-Activated Protein Kinase Contributes to the Early Cardiodepressant Action of Tumor Necrosis Factor J. Am. Coll. Cardiol., August 1, 2006; 48(3): 545 - 555. [Abstract] [Full Text] [PDF]

				E. Arbustini, A. Repetto, M. Pasotti, G. Azan, C. Opasich, C. Campana, A. Gavazzi, R. Ferrari, and L. Tavazzi Cardiomyology: an attempt to link structural cardiac and skeletal muscle damage in patients with dilated cardiomyopathy Eur. Heart J. Suppl., November 1, 2004; 6(suppl_F): F40 - F53. [Abstract] [Full Text] [PDF]

				E. Arbustini, A. Pilotto, A. Repetto, M. Grasso, A. Negri, M. Diegoli, C. Campana, L. Scelsi, E. Baldini, A. Gavazzi, et al. Autosomal dominant dilated cardiomyopathy with atrioventricular block: a lamin A/C defect-related disease J. Am. Coll. Cardiol., March 20, 2002; 39(6): 981 - 990. [Abstract] [Full Text] [PDF]

				C. M. McKee, R. Defina, H. He, K. J. Haley, J. R. Stone, and D. L. Perkins Prolonged Allograft Survival in TNF Receptor 1-Deficient Recipients Is Due to Immunoregulatory Effects, Not to Inhibition of Direct Antigraft Cytotoxicity J. Immunol., January 1, 2002; 168(1): 483 - 489. [Abstract] [Full Text] [PDF]

				M. Azzawi and P. Hasleton Tumour necrosis factor alpha and the cardiovascular system: its role in cardiac allograft rejection and heart disease Cardiovasc Res, September 1, 1999; 43(4): 850 - 859. [Full Text] [PDF]

				D. Li, B. Yang, and J. L. Mehta Tumor necrosis factor-{alpha} enhances hypoxia-reoxygenation-mediated apoptosis in cultured human coronary artery endothelial cells: critical role of protein kinase C Cardiovasc Res, June 1, 1999; 42(3): 805 - 813. [Abstract] [Full Text] [PDF]

				G. Alloatti, C. Penna, A. De Martino, G. Montrucchio, and G. Camussi Role of nitric oxide and platelet-activating factor in cardiac alterations induced by tumor necrosis factor-{alpha} in the guinea-pig papillary muscle Cardiovasc Res, March 1, 1999; 41(3): 611 - 619. [Abstract] [Full Text] [PDF]

				E. Arbustini, M. Diegoli, R. Fasani, M. Grasso, P. Morbini, N. Banchieri, O. Bellini, B. Dal Bello, A. Pilotto, G. Magrini, et al. Mitochondrial DNA Mutations and Mitochondrial Abnormalities in Dilated Cardiomyopathy Am. J. Pathol., November 1, 1998; 153(5): 1501 - 1510. [Abstract] [Full Text] [PDF]

				A. H. Chester, J. A.A. Borland, L. D.K. Buttery, J. A. Mitchell, D. A. Cunningham, S. Hafizi, G. S. Hoare, D. R. Springall, J. M. Polak, and M. H. Yacoub Induction of nitric oxide synthase in human vascular smooth muscle: interactions between proinflammatory cytokines Cardiovasc Res, June 1, 1998; 38(3): 814 - 821. [Abstract] [Full Text] [PDF]

				R. Ferrari Tumor necrosis factor in CHF: a double facet cytokine Cardiovasc Res, March 1, 1998; 37(3): 554 - 559. [Full Text] [PDF]

				C. F. McTiernan, B. H. Lemster, C. Frye, S. Brooks, A. Combes, and A. M. Feldman Interleukin-1ß Inhibits Phospholamban Gene Expression in Cultured Cardiomyocytes Circ. Res., October 19, 1997; 81(4): 493 - 503. [Abstract] [Full Text]

				S. R. Kapadia, H. Oral, J. Lee, M. Nakano, G. E. Taffet, and D. L. Mann Hemodynamic Regulation of Tumor Necrosis Factor-{alpha} Gene and Protein Expression in Adult Feline Myocardium Circ. Res., August 19, 1997; 81(2): 187 - 195. [Abstract] [Full Text]

				H. Oral, G. W. Dorn II, and D. L. Mann Sphingosine Mediates the Immediate Negative Inotropic Effects of Tumor Necrosis Factor-alpha in the Adult Mammalian Cardiac Myocyte J. Biol. Chem., February 21, 1997; 272(8): 4836 - 4842. [Abstract] [Full Text] [PDF]

				D. R. Murray and G. L. Freeman Tumor Necrosis Factor-{alpha} Induces a Biphasic Effect on Myocardial Contractility in Conscious Dogs Circ. Res., January 1, 1996; 78(1): 154 - 160. [Abstract] [Full Text]

				G. Torre-Amione, S. Kapadia, J. Lee, R. D. Bies, R. Lebovitz, and D. L. Mann Expression and Functional Significance of Tumor Necrosis Factor Receptors in Human Myocardium Circulation, September 15, 1995; 92(6): 1487 - 1493. [Abstract] [Full Text]

				C. A. E. O'Blenes, C. Kinnear, and M. Rabinovitch Tumor Necrosis Factor-{alpha} Induces Fibronectin Synthesis in Coronary Artery Smooth Muscle Cells by a Nitric Oxide-Dependent Posttranscriptional Mechanism Circ. Res., July 6, 2001; 89(1): 26 - 32. [Abstract] [Full Text] [PDF]