This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Caamano, J. Articles by Klein-Szanto, A. J. Search for Related Content PubMed PubMed Citation Articles by Caamano, J. Articles by Klein-Szanto, A. J.

American Journal of Pathology, Vol 139, 839-845, Copyright © 1991 by American Society for Investigative Pathology

REGULAR ARTICLES

Detection of p53 in primary lung tumors and nonsmall cell lung carcinoma cell lines

J Caamano, B Ruggeri, S Momiki, A Sickler, SY Zhang and AJ Klein-Szanto
Department of Pathology, Fox Chase Cancer Center, Philadelphia, PA 19111.

Immunohistochemical analysis of p53, a nuclear protein involved in the development of numerous human tumors, was performed in a series of 50 primary nonsmall cell lung carcinomas and in a group of eight lung carcinoma cell lines. Using two mouse monoclonal antibodies, PAb1801 and PAb421, sixteen of thirty-five (45.7%) lung adenocarcinomas and seven of fifteen (46.6%) squamous cell carcinomas showed marked-to- moderate immunoreactivity. In fifty-six percent of the positive tumors more than 40% of all cells were p53 positive, and in only 17% of positive tumors the percentage of immunostained cells was less than ten. Although the number of p53 negative adenocarcinomas without metastasis was larger than the number of p53 positive tumors without metastasis, there were not clear differences between p53 positive and negative tumors with metastasis. Furthermore, six adenocarcinomas that infiltrated the pleura and/or the thoracic wall were p53 positive, whereas only two of these invasive tumors were p53 negative. From eight cell lines studied, six were positive for p53. A good correlation between immunocytochemistry and immunoprecipitation was observed. Two tumorigenic and metastatic cell lines, Calu 1 and Calu 6, that were not immunoreactive also showed lack of protein by immunoprecipitation, as well as absence of mRNA in Northern analysis. In addition, Calu 1 showed an important gene deletion. These observations point to the fact that deletions and alterations in transcription of the p53 gene could coincide with or eventuate in an advanced malignant phenotype that nevertheless results in a p53 negative immunostain. Although this type of change cannot be detected immunohistochemically in primary tumors without further molecular analysis, the results presented herein indicate that p53 can be detected immunohistochemically in a majority of lung tumors and that there is a tendency for more advanced adenocarcinoma stages to exhibit positive p53 immunostain.

This article has been cited by other articles:

				R. Faraonio, P. Vergara, D. Di Marzo, M. G. Pierantoni, M. Napolitano, T. Russo, and F. Cimino p53 Suppresses the Nrf2-dependent Transcription of Antioxidant Response Genes J. Biol. Chem., December 29, 2006; 281(52): 39776 - 39784. [Abstract] [Full Text] [PDF]

				Msh2, Mlh1, Fhit, p53, Bcl-2, and Bax Expression in Invasive and in Situ Squamous Cell Carcinoma of the Uterine Cervix Clin. Cancer Res., September 1, 2000; 6(9): 3600 - 3606. [Abstract] [Full Text]

				O. N. Aurelio, X.-T. Kong, S. Gupta, and E. J. Stanbridge p53 Mutants Have Selective Dominant-Negative Effects on Apoptosis but Not Growth Arrest in Human Cancer Cell Lines Mol. Cell. Biol., February 1, 2000; 20(3): 770 - 778. [Abstract] [Full Text]

				A. Makrigiannakis, K. Amin, G. Coukos, J. L. Tilly, and C. Coutifaris Regulated Expression and Potential Roles of p53 and Wilms' Tumor Suppressor Gene (WT1) during Follicular Development in the Human Ovary J. Clin. Endocrinol. Metab., January 1, 2000; 85(1): 449 - 459. [Abstract] [Full Text]

				G. I. Shapiro, D. A. Koestner, C. B. Matranga, and B. J. Rollins Flavopiridol Induces Cell Cycle Arrest and p53-independent Apoptosis in Non-Small Cell Lung Cancer Cell Lines Clin. Cancer Res., October 1, 1999; 5(10): 2925 - 2938. [Abstract] [Full Text] [PDF]

				Y.-C. Lee, Y.-L. Chang, S.-P. Luh, J.-M. Lee, and J.-S. Chen Significance of P53 and Rb protein expression in surgically treated non-small cell lung cancers Ann. Thorac. Surg., August 1, 1999; 68(2): 343 - 347. [Abstract] [Full Text] [PDF]

				N. Sueoka, H.-Y. Lee, G. L. Walsh, W. K. Hong, and J. M. Kurie Posttranslational Mechanisms Contribute to the Suppression of Specific Cyclin: CDK Complexes by All-Trans Retinoic Acid in Human Bronchial Epithelial Cells Cancer Res., August 1, 1999; 59(15): 3838 - 3844. [Abstract] [Full Text] [PDF]

				M. C. Tammemagi, J. R. McLaughlin, and S. B. Bull Meta-Analyses of p53 Tumor Suppressor Gene Alterations and Clinicopathological Features in Resected Lung Cancers Cancer Epidemiol. Biomarkers Prev., July 1, 1999; 8(7): 625 - 634. [Abstract] [Full Text]

				H. Adachi, G. Preston, B. Harvat, M. I. Dawson, and A. M. Jetten Inhibition of Cell Proliferation and Induction of Apoptosis by the Retinoid AHPN in Human Lung Carcinoma Cells Am. J. Respir. Cell Mol. Biol., March 1, 1998; 18(3): 323 - 333. [Abstract] [Full Text]

				G. I. Shapiro, C. D. Edwards, M. E. Ewen, and B. J. Rollins p16INK4A Participates in a G1 Arrest Checkpoint in Response to DNA Damage Mol. Cell. Biol., January 1, 1998; 18(1): 378 - 387. [Abstract] [Full Text]