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American Journal of Pathology, Vol 139, 961-967, Copyright © 1991 by American Society for Investigative Pathology

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In situ injury-induced release of basic-fibroblast growth factor from corneal epithelial cells

AP Adamis, B Meklir and NC Joyce
Pharmacology Unit, Eye Research Institute, Boston, MA 02114.

Basic-fibroblast growth factor (b-FGF) binds to heparan sulfate proteoglycan in Bowman's layer of the cornea. The mechanism by which the molecule is deposited in Bowman's layer is the subject of controversy since b-FGF lacks a signal peptide sequence for extracellular secretion. Using immunofluorescence, the authors studied the presence and distribution of b-FGF in the bovine cornea and the conditions under which it could be released and bound to Bowman's layer. The results indicate that corneal epithelium contains b-FGF but that uninjured corneas do not contain detectable levels of b-FGF in Bowman's layer. Injury to the corneal epithelium results in the binding of b-FGF to Bowman's layer. Removal of the intact corneal epithelium without cell injury does not result in the binding of b-FGF to Bowman's layer. These findings suggest that one mechanism for the release of b- FGF from corneal epithelial cells is passive leakage after cell injury with secondary binding to Bowman's layer.

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