help button home button Am J Pathol Underwater digital camera with your antibody orders
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kulig, E.
Right arrow Articles by Lloyd, R. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kulig, E.
Right arrow Articles by Lloyd, R. V.

American Journal of Pathology, Vol 140, 207-214, Copyright © 1992 by American Society for Investigative Pathology

REGULAR ARTICLES

The effects of estrogen on prolactin gene methylation in normal and neoplastic rat pituitary tissues

E Kulig, TD Landefeld and RV Lloyd
Department of Pathology, University of Michigan Medical Center, Ann Arbor 48109-0054.

The effects of estrogen treatment on rat prolactin (PRL) gene methylation were analyzed in normal pituitaries and in three transplantable rat pituitary tumors. Northern analysis showed increased PRL mRNA expression in estrogen-treated pituitary and in MtT/F4 and MtT/F-DMBA tumors. Prolactin mRNA amounts in MtT/W15 tumor were decreased by estrogen treatment. There was an inverse relationship between changes in PRL mRNA expression and changes in gene methylation in the coding regions of the PRL gene after estrogen treatment. The amounts of the 4.6-Kb and 1.8-Kb restriction fragments generated by HpaII digestion in pituitary tissues were influenced by estrogen, with an increase in these fragments in normal pituitary, MtT/F4, and MtT/F- DMBA tumors after estrogen treatment. In contrast, the amounts of the 4.6-Kb and 1.8-Kb fragments were decreased in MtT/W15 tumors after estrogen treatment. Most of the internal -CCGG- sites in the PRL gene were methylated in the liver, and the PRL gene was not expressed in the liver. These data suggest that there is a tissue-specific pattern of DNA methylation of the PRL gene and that PRL gene methylation is influenced by estrogen in vivo in normal and tumorous pituitary tissues. These results also suggest that estrogen may influence PRL expression by multiple mechanisms, including changes in the level of DNA methylation.


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
X. Qian, L. Jin, E. Kulig, and R. V. Lloyd
DNA Methylation Regulates p27Kip1 Expression in Rodent Pituitary Cell Lines
Am. J. Pathol., November 1, 1998; 153(5): 1475 - 1482.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
X. Qian, E. Kulig, L. Jin, and R. V. Lloyd
Expression of D-Type Cyclins in Normal and Neoplastic Rat Pituitary
Endocrinology, April 1, 1998; 139(4): 2058 - 2067.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1992 by the American Society for Investigative Pathology.