| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 140, 23-31, Copyright © 1992 by American Society for Investigative Pathology
REGULAR ARTICLES |
BL King, D Carter, HG Foellmer and BM Kacinski
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510.
In this communication, the authors summarize their characterization of eight ovarian adenocarcinoma-derived cell lines for level of neu gene amplification, expression of neu transcripts and protein, and intraperitoneal tumorigenicity in nude mice. Two of the eight cell lines in our study (SKOV3 and YAOVBIX1) exhibited five- to ninefold neu DNA sequence amplification, accompanied by up to 200-fold overexpression of transcripts and protein (p185). Both of these cell lines expressed a major approximately 7.5 kb neu-complementary transcript not previously reported in other neu-positive tumor cell lines. One pair of cell lines (YAOVBIX1 and YAOVBIX3), isolated from a single ovarian carcinoma patient's ascites sample differed dramatically in regard to level of neu gene amplification and expression. Immunohistochemical staining of the primary ovarian tumor from which these two lines were derived demonstrated populations of both neu- positive and neu-negative malignant epithelial cells. Seven of the eight ovarian carcinoma lines produced intra-abdominal tumors after intraperitoneal injection into nude mice, irrespective of level of neu gene expression. This study demonstrates tumor cell heterogeneity with regard to neu gene amplification and expression in an ovarian adenocarcinoma, reveals the overexpression of novel neu-complementary transcripts in two independently isolated ovarian adenocarcinoma cell lines, and suggests that neu gene expression is not required for intraperitoneal tumorigenicity of ovarian carcinoma xenografts in a nude mouse model system.
This article has been cited by other articles:
 |
|
 |
|
  J. J. Schumacher, R. P.M. Dings, J. Cosin, I. V. Subramanian, N. Auersperg, and S. Ramakrishnan Modulation of Angiogenic Phenotype Alters Tumorigenicity in Rat Ovarian Epithelial Cells Cancer Res., April 15, 2007; 67(8): 3683 - 3690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schirmer, J. Kennedy, S. Murli, R. Reid, and D. V. Santi Targeted covalent inactivation of protein kinases by resorcylic acid lactone polyketides. PNAS, March 14, 2006; 103(11): 4234 - 4239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-C. Hsu, H.-C. Chang, and W.-C. Hung HER-2/neu Represses the Metastasis Suppressor RECK via ERK and Sp Transcription Factors to Promote Cell Invasion J. Biol. Chem., February 24, 2006; 281(8): 4718 - 4725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Bonafe, M. Gilmore-Hebert, N. L. Folk, M. Azodi, Y. Zhou, and S. K. Chambers Glyceraldehyde-3-Phosphate Dehydrogenase Binds to the AU-Rich 3' Untranslated Region of Colony-Stimulating Factor-1 (CSF-1) Messenger RNA in Human Ovarian Cancer Cells: Possible Role in CSF-1 Posttranscriptional Regulation and Tumor Phenotype Cancer Res., May 1, 2005; 65(9): 3762 - 3771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Dressman, A. Baras, R. Malinowski, L. B. Alvis, I. Kwon, T. M. Walz, and M. H. Polymeropoulos Gene Expression Profiling Detects Gene Amplification and Differentiates Tumor Types in Breast Cancer Cancer Res., May 1, 2003; 63(9): 2194 - 2199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Chambers and B. M. Kacinski Messenger RNA Decay of Macrophage Colony-Stimulating Factor in Hum a n Ovarian Carcinomas in Vitro Reproductive Sciences, October 1, 1994; 1(4): 310 - 316. [Abstract] [PDF]
|
|
|
|
|
|
J. D. Nathan, D. L. Keefe, M. A. Weinstein, Zhaocong Chen, and F. Naftolin Estrogen Mustard Induces Cell Cycle Arrest of Human Epithelial Ovarian Cancer Cell Lines Reproductive Sciences, January 1, 1994; 1(1): 97 - 103. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
