help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsutsumi, K.
Right arrow Articles by Pater, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsutsumi, K.
Right arrow Articles by Pater, M. M.

American Journal of Pathology, Vol 140, 255-261, Copyright © 1992 by American Society for Investigative Pathology

REGULAR ARTICLES

Human papillomavirus 16 DNA immortalizes two types of normal human epithelial cells of the uterine cervix

K Tsutsumi, N Belaguli, S Qi, TI Michalak, WP Gulliver, A Pater and MM Pater
Division of Basic Medical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.

Premalignant cervical lesions occur at the squamo-columnar junction and in endocervical epithelium and squamous ectocervical epithelium, in descending order of frequency. However, previously only ectocervical cells have been clearly shown to be immortalized in vitro by the oncogenic human papillomaviruses (HPVs). This report describes the immortalization of normal human ecto- and endocervical epithelial cells by the intact HPV 16 genome. Ectocervical epithelial cells (HEC) became immortalized (HEC-16) without crisis while endocervical cells (HEN) were immortalized (HEN-16) after undergoing crisis. HEN-16 and HEC-16 contained integrated HPV 16 DNA, expressed E6 and E7 mRNA, and were aneuploid and nontumorigenic. They also expressed cytokeratins in a pattern similar to their distinct normal parental cells. These results suggest that both squamous and simple epithelial cells of uterine cervix are targets for immortalization by HPV 16.


This article has been cited by other articles:


Home page
 Obstet GynecolHome page
M. Yokoyama, Y. Nakao, T. Iwasaka, A. Pater, and H. Sugimori
Retinoic Acid and Interferon-{alpha} Effects on Cell Growth and Differentiation in Cervical Carcinoma Cell Lines
Obstet. Gynecol., August 1, 2001; 98(2): 332 - 340.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1992 by the American Society for Investigative Pathology.