This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rohde, D. Articles by Lemke, H. Search for Related Content PubMed PubMed Citation Articles by Rohde, D. Articles by Lemke, H.

American Journal of Pathology, Vol 140, 473-482, Copyright © 1992 by American Society for Investigative Pathology

REGULAR ARTICLES

Cellular localizations and processing of the two molecular forms of the Hodgkin-associated Ki-1 (CD30) antigen. The protein kinase Ki-1/57 occurs in the nucleus

D Rohde, H Hansen, M Hafner, H Lange, V Mielke, ML Hansmann and H Lemke
Biochemisches Instituts, Medizinischen Fakultat, Christian-Albrechts- Universitat zu Kiel, Federal Republic of Germany.

The Ki-1 antibody not only detects a Hodgkin-associated membrane molecule of 120 kd (Ki-1/120 = CD30), but also reacts with an independently synthesized molecule of 57 kd (Ki-1/57) that only occurs intracellularly. Hodgkin's disease-derived cell lines L428 and L540 contain both Ki-1-reactive antigens, whereas others, e.g., U266/Bl myeloma cells, only express the intracellular Ki-1/57. The present immunoelectronmicroscopic analysis detected the Ki-1/57 antigen of U266/Bl cells not only in the cytoplasm, but also in association with the nuclear envelope, chromatin structures, and nucleoli. This Ki-1/57- specific type of labeling also was observed in L428 and L540 cells that, in contrast to U266/Bl cells, showed an additional staining of cell membranes and cytoplasmic vesicles. These results were confirmed by two independent methods: 1) cytocentrifuge preparations of isolated nuclei of L540 cells showed a spotted Ki-1-specific labeling, 2) immunoprecipitations demonstrated that the Ki-1/57, but not the Ki- 1/120 antigen, was transferred into the nuclei of L540 and U266/Bl cells, whereas the Ki-1/120 antigen with its 90-kd precursor remained in the non-nuclei fraction of L540 cells.

This article has been cited by other articles:

				F. C. Nery, D. O. Passos, V. S. Garcia, and J. Kobarg Ki-1/57 Interacts with RACK1 and Is a Substrate for the Phosphorylation by Phorbol 12-Myristate 13-Acetate-activated Protein Kinase C J. Biol. Chem., March 19, 2004; 279(12): 11444 - 11455. [Abstract] [Full Text] [PDF]

				V. Gattei, M. Degan, A. Gloghini, A. De Iuliis, S. Improta, F. M. Rossi, D. Aldinucci, V. Perin, D. Serraino, R. Babare, et al. CD30 Ligand Is Frequently Expressed in Human Hematopoietic Malignancies of Myeloid and Lymphoid Origin Blood, March 15, 1997; 89(6): 2048 - 2059. [Abstract] [Full Text] [PDF]

				L. Huang, N. Grammatikakis, M. Yoneda, S. D. Banerjee, and B. P. Toole Molecular Characterization of a Novel Intracellular Hyaluronan-binding Protein J. Biol. Chem., September 15, 2000; 275(38): 29829 - 29839. [Abstract] [Full Text] [PDF]