| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 140, 795-807, Copyright © 1992 by American Society for Investigative Pathology
REGULAR ARTICLES |
AM Dvorak, SJ Ackerman, T Furitsu, P Estrella, L Letourneau and T Ishizaka
Department of Pathology, Beth Israel Hospital, Boston, MA 02215.
The mechanism of piecemeal degranulation by human eosinophils was investigated. Mature eosinophils that developed in rhIL-5-containing conditioned media from cultured human cord blood mononuclear cells were prepared for ultrastructural studies using a combined technique to image eosinophil peroxidase by cytochemistry in the same sections on which postembedding immunogold was used to demonstrate Charcot-Leyden crystal protein. Vesicular transport of eosinophil peroxidase from the specific granule matrix compartment to the cell surface was associated with piecemeal degranulation. This process involved budding of eosinophil peroxidase-loaded vesicles and tubules from specific granules. Some eosinophil peroxidase that was released from eosinophils remained bound to the cell surface; some was free among the cultured cells. Macrophages and basophils bound the released eosinophil peroxidase to their plasma membranes, internalized it in endocytotic vesicles, and stored it in their respective phagolysosomes and secretory granules. Charcot-Leyden crystal protein was diffusely present in the nucleus and cytoplasm of IL-5-stimulated mature eosinophils. Extensive amounts were generally present in granule-poor and subplasma membrane areas of the cytoplasm in contrast to eosinophil peroxidase, which was secreted and bound to the external surface of eosinophil plasma membranes. These studies establish vesicular transport as a mechanism for emptying the specific eosinophil granule matrix compartment during IL-5-associated piecemeal degranulation.
This article has been cited by other articles:
 |
|
 |
|
  R. C. N. Melo, L. A. Spencer, A. M. Dvorak, and P. F. Weller Mechanisms of eosinophil secretion: large vesiculotubular carriers mediate transport and release of granule-derived cytokines and other proteins J. Leukoc. Biol., February 1, 2008; 83(2): 229 - 236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Clark, L. Simson, N. Newcombe, A. M. L. Koskinen, J. Mattes, N. A. Lee, J. J. Lee, L. A. Dent, K. I. Matthaei, and P. S. Foster Eosinophil degranulation in the allergic lung of mice primarily occurs in the airway lumen J. Leukoc. Biol., June 1, 2004; 75(6): 1001 - 1009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mahmudi-Azer, G. P. Downey, and R. Moqbel Translocation of the tetraspanin CD63 in association with human eosinophil mediator release Blood, May 13, 2002; 99(11): 4039 - 4047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Feng, R. Flaumenhaft, C. Bandeira–Melo, P. F. Weller, and A. M. Dvorak Ultrastructural Localization of Vesicle-associated Membrane Protein(s) to Specialized Membrane Structures in Human Pericytes, Vascular Smooth Muscle Cells, Endothelial Cells, Neutrophils, and Eosinophils J. Histochem. Cytochem., March 1, 2001; 49(3): 293 - 304. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Karawajczyk, L. Sevéus, R. Garcia, E. Björnsson, C. G. B. Peterson, G. M. Roomans, and P. Venge Piecemeal Degranulation of Peripheral Blood Eosinophils . A Study of Allergic Subjects during and out of the Pollen Season Am. J. Respir. Cell Mol. Biol., October 1, 2000; 23(4): 521 - 529. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. A. Giembycz and M. A. Lindsay Pharmacology of the Eosinophil Pharmacol. Rev., June 1, 1999; 51(2): 213 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Scepek and M. Lindau Exocytotic Competence and Intergranular Fusion in Cord Blood-Derived Eosinophils During Differentiation Blood, January 15, 1997; 89(2): 510 - 517. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
