This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kono, D. H. Articles by Theofilopoulos, A. N. Search for Related Content PubMed PubMed Citation Articles by Kono, D. H. Articles by Theofilopoulos, A. N.

American Journal of Pathology, Vol 140, 823-830, Copyright © 1992 by American Society for Investigative Pathology

REGULAR ARTICLES

Application of a multiprobe RNase protection assay and junctional sequences to define V beta gene diversity in Sezary syndrome

DH Kono, R Baccala, RS Balderas, SJ Kovac, PW Heald, RL Edelson and AN Theofilopoulos
Department of Immunology, Scripps Research Institute, La Jolla, California.

Nineteen patients with mycosis fungoides/Sezary syndrome (MF/SZ), a malignancy of the mature helper T-cell phenotype (CD4+TCR alpha beta+), were screened for clonotypic V beta expansions in peripheral blood with a multiprobe RNase protection assay. A different predominant V beta gene was identified in 9 of 14 patients with high peripheral blood CD4/CD8 ratios, whereas 4 of these patients showed T-cell expansions expressing V beta genes other than those included in the assay. In contrast, five patients with few, if any, malignant cells in the circulation had V beta expression levels similar to that in normal peripheral blood. A unique V-D-J sequence was found for each highly expressed V beta gene, thereby documenting monoclonality of the expanded T-cell populations. Polymerase chain reaction (PCR) primers specific for the D-J beta junction accurately identified the corresponding malignant clonotype in peripheral blood. The diverse TCR V beta gene usage found in these MF/SZ patients suggests that T-cell receptor (TCR) specificity has no bearing on this disease.

This article has been cited by other articles:

				C. Assaf, M. Hummel, E. Dippel, S. Goerdt, H.-H. Muller, I. Anagnostopoulos, C. E. Orfanos, and H. Stein High detection rate of T-cell receptor beta chain rearrangements in T-cell lymphoproliferations by family specific polymerase chain reaction in combination with the GeneScan technique and DNA sequencing Blood, July 15, 2000; 96(2): 640 - 646. [Abstract] [Full Text] [PDF]

				C. M. Jackow, J. C. Cather, V. Hearne, A. T. Asano, J. M. Musser, and M. Duvic Association of Erythrodermic Cutaneous T-Cell Lymphoma, Superantigen-Positive Staphylococcus aureus, and Oligoclonal T-Cell Receptor Vbeta Gene Expansion Blood, January 1, 1997; 89(1): 32 - 40. [Abstract] [Full Text] [PDF]