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American Journal of Pathology, Vol 140, 1025-1030, Copyright © 1992 by American Society for Investigative Pathology

REGULAR ARTICLES

Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody

A Hameed, KJ Olsen, L Cheng, WM Fox 3d, RH Hruban and ER Podack
Department of Pathology, Johns Hopkins Hospital, Baltimore, Maryland 21205.

Perforin is a potent cytolytic pore-forming protein expressed in cytoplasmic granules of cytotoxic T lymphocytes and natural killer cells. A new monoclonal antibody raised against human perforin was used to detect both in vitro and in vivo perforin expression in cytotoxic cells. Immunohistochemical analysis of human peripheral blood mononuclear cells cultured in recombinant interleukin-2 (rIL-2) showed strong granular cytoplasmic staining of the IL-2 activated cytotoxic cells. Fresh-frozen tissue sections from patients with heart allograft rejection were also stained. Strong granular cytoplasmic staining of the mononuclear inflammatory infiltrate characteristic for perforin in cardiac allograft rejection was observed. The detection and quantitative analysis of perforin-associated cytotoxic cells by the human anti-perforin monoclonal antibody will help to evaluate the significance of these functionally distinct cytotoxic cells in human tissue.

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