| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 141, 397-407, Copyright © 1992 by American Society for Investigative Pathology
REGULAR ARTICLES |
RM Strieter, K Kasahara, RM Allen, TJ Standiford, MW Rolfe, FS Becker, SW Chensue and SL Kunkel
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0360.
During acute inflammation, the first line of cellular response for host defense is the neutrophil. In addition to the historic role of the neutrophil as a phagocyte, recent studies have identified this cell as an important source of a number of cytokines. In this study, we provide evidence that the neutrophil is a significant source of interleukin-8 (IL-8). Neutrophils freshly isolated from whole blood were not found to constitutively express IL-8 mRNA. In contrast, when these leukocytes were cultured on plastic they were activated, leading to the significant expression of de novo steady-state levels of IL-8 mRNA. In addition, when neutrophils were treated with cycloheximide, there was evidence for "superinduction" of steady-state levels of IL-8 mRNA and inhibition of antigenic IL-8 production. Neutrophils were subsequently stimulated with lipopolysaccharide (LPS), tumor necrosis factor-alpha, or interleukin-1-beta and were found to express IL-8 mRNA and antigen in both a time- and dose-dependent manner. Furthermore, neutrophils stimulated with traditional chemotactic/activating factors, such as the split product of the fifth component of complement (C5a), formylmethionyleucylphenylalanine (fMLP), and leukotriene B4 (LTB4) in a dose-dependent manner did not produce significant antigenic IL-8, as compared with unstimulated controls. In contrast, when neutrophils were exposed to either of these neutrophil agonists in the presence of LPS, the production of antigenic IL-8 was significantly elevated, as compared with either of the stimuli alone, suggesting a synergistic response. These data would suggest that the neutrophil can no longer be viewed as only a phagocyte or warehouse for proteolytic enzymes, but is a pivotal effector cell that is able to respond to mediators in its environment and generate cytokines. This latter neutrophil response may be important for either the elicitation of additional neutrophils or to orchestrate the conventional immune response at sites of inflammation.
This article has been cited by other articles:
 |
|
 |
|
  R.-F. Guo, L. Sun, H. Gao, K. X. Shi, D. Rittirsch, V. J. Sarma, F. S. Zetoune, and P. A. Ward In vivo regulation of neutrophil apoptosis by C5a during sepsis J. Leukoc. Biol., December 1, 2006; 80(6): 1575 - 1583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wislez, N. Fujimoto, J. G. Izzo, A. E. Hanna, D. D. Cody, R. R. Langley, H. Tang, M. D. Burdick, M. Sato, J. D. Minna, et al. High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras. Cancer Res., April 15, 2006; 66(8): 4198 - 4207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Jo, H.-Y. Lee, Y.-N. Lee, J. I. Kim, H.-K. Kang, D.-W. Park, S.-H. Baek, J.-Y. Kwak, and Y.-S. Bae Group IB Secretory Phospholipase A2 Stimulates CXC Chemokine Ligand 8 Production via ERK and NF-{kappa}B in Human Neutrophils J. Immunol., November 15, 2004; 173(10): 6433 - 6439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. G. Arndt, N. Suzuki, N. J. Avdi, K. C. Malcolm, and G. S. Worthen Lipopolysaccharide-induced c-Jun NH2-terminal Kinase Activation in Human Neutrophils: ROLE OF PHOSPHATIDYLINOSITOL 3-KINASE AND Syk-MEDIATED PATHWAYS J. Biol. Chem., March 19, 2004; 279(12): 10883 - 10891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Ryu, J.-H. Kang, S.-Y. Jung, M.-H. Shin, J.-M. Kim, H. Park, and D.-Y. Min Production of Interleukin-8 by Human Neutrophils Stimulated with Trichomonas vaginalis Infect. Immun., March 1, 2004; 72(3): 1326 - 1332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. C. Riedemann, R.-F. Guo, and P. A. Ward A key role of C5a/C5aR activation for the development of sepsis J. Leukoc. Biol., December 1, 2003; 74(6): 966 - 970. [Abstract] [Full Text]
|
|
|
|
|
|
A. Cloutier, T. Ear, O. Borissevitch, P. Larivee, and P. P. McDonald Inflammatory Cytokine Expression Is Independent of the c-Jun N-Terminal Kinase/AP-1 Signaling Cascade in Human Neutrophils J. Immunol., October 1, 2003; 171(7): 3751 - 3761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Hayashi, T. K. Means, and A. D. Luster Toll-like receptors stimulate human neutrophil function Blood, October 1, 2003; 102(7): 2660 - 2669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Sabroe, L. R. Prince, E. C. Jones, M. J. Horsburgh, S. J. Foster, S. N. Vogel, S. K. Dower, and M. K. B. Whyte Selective Roles for Toll-Like Receptor (TLR)2 and TLR4 in the Regulation of Neutrophil Activation and Life Span J. Immunol., May 15, 2003; 170(10): 5268 - 5275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Bosshart and M. Heinzelmann Arginine-Rich Cationic Polypeptides Amplify Lipopolysaccharide-Induced Monocyte Activation Infect. Immun., December 1, 2002; 70(12): 6904 - 6910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ersson, M. Walles, K. Ohlsson, and A. Ekholm Chronic hyperbaric exposure activates proinflammatory mediators in humans J Appl Physiol, June 1, 2002; 92(6): 2375 - 2380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Sabroe, E. C. Jones, L. R. Usher, M. K. B. Whyte, and S. K. Dower Toll-Like Receptor (TLR)2 and TLR4 in Human Peripheral Blood Granulocytes: A Critical Role for Monocytes in Leukocyte Lipopolysaccharide Responses J. Immunol., May 1, 2002; 168(9): 4701 - 4710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Muller-Suur, P.H. Larsson, K. Larsson, and J. Grunewald Lymphocyte activation after exposure to swine dust: a role of humoral mediators and phagocytic cells Eur. Respir. J., January 1, 2002; 19(1): 104 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. White, D. L. Livant, S. Markwart, and D. A. Arenberg Monocyte-Fibronectin Interactions, Via {alpha}5{beta}1 Integrin, Induce Expression of CXC Chemokine-Dependent Angiogenic Activity J. Immunol., November 1, 2001; 167(9): 5362 - 5366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Chensue Molecular Machinations: Chemokine Signals in Host-Pathogen Interactions Clin. Microbiol. Rev., October 1, 2001; 14(4): 821 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ethuin, C. Delarche, S. Benslama, M.-A. Gougerot-Pocidalo, L. Jacob, and S. Chollet-Martin Interleukin-12 increases interleukin 8 production and release by human polymorphonuclear neutrophils J. Leukoc. Biol., September 1, 2001; 70(3): 439 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Qiu, K. A. Frait, F. Reich, E. Komuniecki, and S. W. Chensue Chemokine Expression Dynamics in Mycobacterial (Type-1) and Schistosomal (Type-2) Antigen-Elicited Pulmonary Granuloma Formation Am. J. Pathol., April 1, 2001; 158(4): 1503 - 1515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Savoie, V. Lavastre, M. Pelletier, T. Hajto, K. Hostanska, and D. Girard Activation of human neutrophils by the plant lectin Viscum album agglutinin-I: modulation of de novo protein synthesis and evidence that caspases are involved in induction of apoptosis J. Leukoc. Biol., December 1, 2000; 68(6): 845 - 853. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. M. Page, G. J. Gleich, K. A. Roebuck, and L. L. Thomas Stimulation of Neutrophil Interleukin-8 Production by Eosinophil Granule Major Basic Protein Am. J. Respir. Cell Mol. Biol., August 1, 1999; 21(2): 230 - 237. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Hatano, T. Kasama, H. Iwabuchi, R. Hanaoka, H. T Takeuchi, L. Jing, Y. Mori, K. Kobayashi, M. Negishi, H. Ide, et al. Macrophage inflammatory protein 1 alpha expression by synovial fluid neutrophils in rheumatoid arthritis Ann Rheum Dis, May 1, 1999; 58(5): 297 - 302. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. E John, J. Galea, S. E Francis, C. M Holt, and A. Finn Interleukin-8 mRNA expression in circulating leucocytes during cardiopulmonary bypass Perfusion, December 1, 1998; 13(6): 409 - 417. [Abstract] [PDF]
|
|
|
|
|
|
M. Heinzelmann, M. A. Mercer-Jones, H. Flodgaard, and F. N. Miller Heparin-Binding Protein (CAP37) Is Internalized in Monocytes and Increases LPS-Induced Monocyte Activation J. Immunol., June 1, 1998; 160(11): 5530 - 5536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Marie, J. Muret, C. Fitting, M.-R. Losser, D. Payen, and J.-M. Cavaillon Reduced Ex Vivo Interleukin-8 Production by Neutrophils in Septic and Nonseptic Systemic Inflammatory Response Syndrome Blood, May 1, 1998; 91(9): 3439 - 3446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Palmberg, B.-M. Larsson, P. Malmberg, and K. Larsson Induction of IL-8 production in human alveolar macrophages and human bronchial epithelial cells in vitro by swine dust Thorax, April 1, 1998; 53(4): 260 - 264. [Abstract] [Full Text]
|
|
|
|
|
|
J. Qi, S. Goralnick, and D. L. Kreutzer Fibrin Regulation of Interleukin-8 Gene Expression in Human Vascular Endothelial Cells Blood, November 1, 1997; 90(9): 3595 - 3602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. McDonald, A. Bald, and M. A. Cassatella Activation of the NF-kappa B Pathway by Inflammatory Stimuli in Human Neutrophils Blood, May 1, 1997; 89(9): 3421 - 3433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Browning, Z. K. Pan, E. R. Prossnitz, and R. D. Ye Cell Type- and Developmental Stage-specific Activation of NF-kappa B by fMet-Leu-Phe in Myeloid Cells J. Biol. Chem., March 21, 1997; 272(12): 7995 - 8001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Novick, K. E. Gehman, I. S. Ali, and J. Lee Lung Preservation: The Importance of Endothelial and Alveolar Type II Cell Integrity Ann. Thorac. Surg., July 1, 1996; 62(1): 302 - 314. [Abstract] [Full Text]
|
|
|
|
|
|
B. B. Damaj, S. R. McColl, W. Mahana, M. F. Crouch, and P. H. Naccache Physical Association of Gi2alpha with Interleukin-8 Receptors J. Biol. Chem., May 31, 1996; 271(22): 12783 - 12789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Chertov, D. F. Michiel, L. Xu, J. M. Wang, K. Tani, W. J. Murphy, D. L. Longo, D. D. Taub, and J. J. Oppenheim Identification of Defensin-1, Defensin-2, and CAP37/Azurocidin as T-cell Chemoattractant Proteins Released from Interleukin-8-stimulated Neutrophils J. Biol. Chem., February 9, 1996; 271(6): 2935 - 2940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. W. Wakefield, R. M. Strieter, C. A. Wilke, A. M. Kadell, S. K. Wrobleski, M. D. Burdick, R. Schmidt, S. L. Kunkel, and L. J. Greenfield Venous Thrombosis–Associated Inflammation and Attenuation With Neutralizing Antibodies to Cytokines and Adhesion Molecules Arterioscler. Thromb. Vasc. Biol., February 1, 1995; 15(2): 258 - 268. [Abstract] [Full Text]
|
|
|
|
|
|
I. Vancurova, V. Miskolci, and D. Davidson NF-kappa B Activation in Tumor Necrosis Factor alpha -stimulated Neutrophils Is Mediated by Protein Kinase Cdelta . CORRELATION TO NUCLEAR Ikappa Balpha J. Biol. Chem., June 1, 2001; 276(23): 19746 - 19752. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
