| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 141, 685-690, Copyright © 1992 by American Society for Investigative Pathology
REGULAR ARTICLES |
U Zeymer, MC Fishbein, JS Forrester and B Cercek
Division of Cardiology, Cedars Sinai Medical Center, Los Angeles, California 90048-0750.
The authors compared detection of proliferating vascular smooth muscle cells (SMC) in vitro and in vivo with proliferating cell nuclear antigen (PCNA) immunohistochemistry and two established methods: [3H]thymidine autoradiography and bromodeoxyuridine immunohistochemistry. Labeling with [3H]thymidine and bromodeoxyuridine of rat vascular SMC in culture stained 11% +/- 2% and 11% +/- 1% of cells, and PCNA immunohistochemistry 22% +/- 2% of cells. Proliferation in the media of the denuded rat aortae was highest 3 days after denudation: 4.2%, 3.8%, and 4.7% of labeled cells with [3H]thymidine, bromodeoxyuridine, and PCNA, respectively. In the intima, proliferation was highest 7 days after denudation with 42% [3H]thymidine, 40% bromodeoxyuridine, and 46% PCNA-positive cells. With double labeling, all [3H]thymidine-positive cells were PCNA positive, whereas some cells were only positive for PCNA. The authors conclude that PCNA immunohistochemistry compares favorably with [3H]thymidine autoradiography, and bromodeoxyuridine immunohistochemistry.
This article has been cited by other articles:
 |
|
 |
|
  W. Yang, J. Ge, H. Liu, K. Zhao, X. Liu, X. Qu, W. Li, Y. Huang, A. Sun, and Y. Zou Arsenic trioxide eluting stent reduces neointima formation in a rabbit iliac artery injury model Cardiovasc Res, December 1, 2006; 72(3): 483 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zargham and G. Thibault {alpha}8{beta}1 Integrin expression in the rat carotid artery: involvement in smooth muscle cell migration and neointima formation Cardiovasc Res, March 1, 2005; 65(4): 813 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Li, P. Dimayuga, M. Yamashita, J. Yano, M. Fournier, M. Lewis, and B. Cercek Arterial Injury in Mice with Severe Insulin-Like Growth Factor-1 (IGF-1) Deficiency Journal of Cardiovascular Pharmacology and Therapeutics, December 1, 2002; 7(4): 227 - 233. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Hay, C. Micko, M. F. Prescott, G. Liau, K. Robinson, and H. De Leon Differential Cell Cycle Progression Patterns of Infiltrating Leukocytes and Resident Cells After Balloon Injury of the Rat Carotid Artery Arterioscler. Thromb. Vasc. Biol., December 1, 2001; 21(12): 1948 - 1954. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-Y. Fan, K. S. Ramos, and R. S. Chapkin Dietary {{gamma}}-Linolenic Acid Suppresses Aortic Smooth Muscle Cell Proliferation and Modifies Atherosclerotic Lesions in Apolipoprotein E Knockout Mice J. Nutr., June 1, 2001; 131(6): 1675 - 1681. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Tsukamoto, N. Matsuo, K. Ozawa, O. Hori, T. Higashi, J. Nishizaki, N. Tohnai, I. Nagata, K. Kawano, C. Yutani, et al. Expression of a Novel RNA-Splicing Factor, RA301/Tra2{beta}, in Vascular Lesions and Its Role in Smooth Muscle Cell Proliferation Am. J. Pathol., May 1, 2001; 158(5): 1685 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Stawowy, J. Marcinkiewicz, K. Graf, N. G. Seidah, M. Chrétien, E. Fleck, and M. Marcinkiewicz Selective Expression of the Proprotein Convertases Furin, PC5, and PC7 in Proliferating Vascular Smooth Muscle Cells of the Rat Aorta In Vitro J. Histochem. Cytochem., March 1, 2001; 49(3): 323 - 332. [Abstract] [Full Text]
|
|
|
|
|
|
A. M. Holm, C. B. Andersen, S. Haunso, and P. Riis Hansen ACE-inhibition promotes apoptosis after balloon injury of rat carotid arteries Cardiovasc Res, February 1, 2000; 45(3): 777 - 782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. SALMON, H. KOTO, O. T. LYNCH, E.-B. HADDAD, N. J. LAMB, G. J. QUINLAN, P. J. BARNES, and K. FAN CHUNG Proliferation of Airway Epithelium After Ozone Exposure . Effect of Apocynin and Dexamethasone Am. J. Respir. Crit. Care Med., March 1, 1998; 157(3): 970 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Brandl, T. Richter, K. Haug, M. G. Wilhelm, P. C. Maurer, and W. Nathrath Topographic Analysis of Proliferative Activity in Carotid Endarterectomy Specimens by Immunocytochemical Detection of the Cell Cycle–Related Antigen Ki-67 Circulation, November 18, 1997; 96(10): 3360 - 3368. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kollum, S. Kaiser, R. Kinscherf, J. Metz, W. Kubler, and C. Hehrlein Apoptosis After Stent Implantation Compared With Balloon Angioplasty in Rabbits : Role of Macrophages Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 2383 - 2388. [Abstract] [Full Text]
|
|
|
|
|
|
L. Capron, J. Jarnet, D. Heudes, D. Joseph-Monrose, and P. Bruneval Repeated Balloon Injury of Rat Aorta : A Model of Neointima With Attenuated Inhibition by Heparin Arterioscler. Thromb. Vasc. Biol., September 1, 1997; 17(9): 1649 - 1656. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Aoyagi, M. Yamamoto, H. Wakimoto, H. Azuma, K. Hirakawa, and K. Yamamoto Immunohistochemical Detection of Ki-67 in Replicative Smooth Muscle Cells of Rabbit Carotid Arteries After Balloon Denudation Stroke, December 1, 1995; 26(12): 2328 - 2332. [Abstract] [Full Text]
|
|
|
|
|
|
V. Verin, Y. Popowski, P. Urban, J. Belenger, M. Redard, M. Costa, M.-C. Widmer, M. Rouzaud, P. Nouet, E. Grob, et al. Intra-arterial Beta Irradiation Prevents Neointimal Hyperplasia in a Hypercholesterolemic Rabbit Restenosis Model Circulation, October 15, 1995; 92(8): 2284 - 2290. [Abstract] [Full Text]
|
|
|
|
|
|
D. Zahger, M. C. Fishbein, L. I. Garfinkel, P. K. Shah, J. S. Forrester, J. Regnstrom, J. Yano, and B. Cercek VCL, an Antagonist of the Platelet GP1b Receptor, Markedly Inhibits Platelet Adhesion and Intimal Thickening After Balloon Injury in the Rat Circulation, September 1, 1995; 92(5): 1269 - 1273. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
