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American Journal of Pathology, Vol 141, 699-706, Copyright © 1992 by American Society for Investigative Pathology

REGULAR ARTICLES

Assessment of proliferative activity in ovarian neoplasms by flow and static cytometry. Correlation with prognostic features

PC Huettner, DS Weinberg and JM Lage
Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.

We undertook a prospective flow and static cytometric study of proliferative activity in 74 malignant, borderline and benign ovarian neoplasms. Proliferative activity as assessed by S phase fraction (SPF), and immunostaining of tissue sections with Ki-67 antibody was compared with prognostically important clinicopathologic features. Malignant neoplasms had higher median percentage Ki-67 staining (27.6%) than borderline (12.3%) and benign (2.7%) tumors (P less than 0.05). Percentage Ki-67 staining correlated with SPF, DNA index, architectural grade, nucleolar grade, and mitotic count in malignant tumors; with nucleolar grade in benign tumors and with none of these variables in borderline tumors. Similarly, malignant neoplasms had a higher median SPF (11.5%) than borderline (3.4%) and benign (2.9%) neoplasms (P less than 0.05). In malignant neoplasms, SPF correlated with percentage Ki- 67 staining, DNA index, age, and stage, but with none of these in borderline or benign neoplasms.