| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 141, 1063-1072, Copyright © 1992 by American Society for Investigative Pathology
REGULAR ARTICLES |
C van Kalken, G Giaccone, P van der Valk, CM Kuiper, MM Hadisaputro, SA Bosma, RJ Scheper, CJ Meijer and HM Pinedo
Department of Medical Oncology, Free University Hospital, Amsterdam, The Netherlands.
P-glycoprotein, a transmembrane protein associated with multidrug resistance in cancer cells, is also expressed in normal tissues. To get more insight into the physiologic role of mdr1/P-glycoprotein, we investigated its expression in human fetal tissues after 7 to 38 weeks of gestation by an immunohistochemical technique, using three different monoclonal antibodies, and by a sensitive RNAse protection assay. Expression of mdr1-mRNA could already be demonstrated in the embryonal phase of human development, after 7 weeks of gestation. Comparing the adult with the fetal tissue distribution, differences were found in specific organs, such as adrenal, intestine, respiratory epithelium, and brain capillaries. In the fetal zone cells of the fetal adrenal cortex no staining was observed by immunohistochemistry, whereas the definitive zone showed increasing expression throughout gestation. Prenatal intestine did not show staining of the epithelium, although definite mdr1-mRNA expression was observed in late specimens. Interestingly, respiratory epithelium of main bronchi and pharynx, not expressing P-gp in adults, did stain positive. Expression of P-gp in brain capillaries was not observed before the third trimester of pregnancy, whereas in kidney and liver, mdr1-mRNA expression and staining for P-glycoprotein were detected in early fetal life (11 to 14 weeks). These findings suggest a pivotal role of P-glycoprotein in physiology of various organs already in early phases of human development and may help to identify its physiologic substrates.
This article has been cited by other articles:
 |
|
 |
|
  S. Petropoulos, G. M. Kalabis, W. Gibb, and S. G. Matthews Functional Changes of Mouse Placental Multidrug Resistance Phosphoglycoprotein (ABCB1) With Advancing Gestation and Regulation by Progesterone Reproductive Sciences, May 1, 2007; 14(4): 321 - 328. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Couture, J. A. Nash, and J. Turgeon The ATP-Binding Cassette Transporters and Their Implication in Drug Disposition: A Special Look at the Heart. Pharmacol. Rev., June 1, 2006; 58(2): 244 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fakhoury, C. Litalien, Y. Medard, H. Cave, N. Ezzahir, M. Peuchmaur, and E. Jacqz-Aigrain LOCALIZATION AND mRNA EXPRESSION OF CYP3A AND P-GLYCOPROTEIN IN HUMAN DUODENUM AS A FUNCTION OF AGE Drug Metab. Dispos., November 1, 2005; 33(11): 1603 - 1607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Kalabis, A. Kostaki, M. H. Andrews, S. Petropoulos, W. Gibb, and S. G. Matthews Multidrug Resistance Phosphoglycoprotein (ABCB1) in the Mouse Placenta: Fetal Protection Biol Reprod, October 1, 2005; 73(4): 591 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ratcliffe, M. Nakanishi, and R. B. Jaffe Identification of Definitive and Fetal Zone Markers in the Human Fetal Adrenal Gland Reveals Putative Developmental Genes J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3272 - 3277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Yague, A. L. Armesilla, G. Harrison, J. Elliott, A. Sardini, C. F. Higgins, and S. Raguz P-glycoprotein (MDR1) Expression in Leukemic Cells Is Regulated at Two Distinct Steps, mRNA Stabilization and Translational Initiation J. Biol. Chem., March 14, 2003; 278(12): 10344 - 10352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Giaccone and H. M. Pinedo Drug Resistance Oncologist, February 1, 1996; 1(1): 82 - 87. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
