| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 142, 3-9, Copyright © 1993 by American Society for Investigative Pathology
REGULAR ARTICLES |
H Kreipe, HJ Heidebrecht, S Hansen, W Rohlk, M Kubbies, HH Wacker, M Tiemann, HJ Radzun and R Parwaresch
Institute of Pathology and Hematopathology, University of Kiel, Germany.
A monoclonal antibody (Ki-S1) has been raised that reacts with the nuclei of proliferating cells. The antigen recognized is resistant to formalin fixation and can be detected in frozen tissues as well as in routinely processed specimens. In immunohistochemistry, nuclear staining can be seen in those tissues and cellular compartments known to be actively proliferating. Peripheral blood lymphocytes are negative but show a strong increase in antigen expression after mitogen stimulation. Flow cytometric determination of DNA content and antigen expression revealed negativity of G0 cells and positivity of G1 to G2/M cells. A cytoplasmic co-reactivity, not associated with proliferation, was confined to Langerhans islands of the pancreas. The nuclear localized antigen has a molecular mass of 160 kd and therefore seems to be different from all other known immunohistochemical markers of proliferating cells. We conclude that the monoclonal antibody Ki-S1 might provide a useful tool for studying cell proliferation in situ under normal and pathological circumstances.
This article has been cited by other articles:
 |
|
 |
|
  C. CORDES, A.-K. MUNZEL, P. RUDOLPH, M. HOFFMANN, I. LEUSCHNER, and S. GOTTSCHLICH Immunohistochemical Staining of Ki-67 Using the Monoclonal Antibody Ki-S11 Is a Prognostic Indicator for Laryngeal Squamous Cell Carcinoma Anticancer Res, April 1, 2009; 29(4): 1459 - 1465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. A. Doussis-Anagnostopoulou, T. P. Vassilakopoulos, I. Thymara, P. Korkolopoulou, M. K. Angelopoulou, M. P. Siakantaris, S. I. Kokoris, E. M. Dimitriadou, C. Kalpadakis, M. Matzouranis, et al. Topoisomerase II{alpha} Expression as an Independent Prognostic Factor in Hodgkin's Lymphoma Clin. Cancer Res., March 15, 2008; 14(6): 1759 - 1766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Greeve, A. Philipsen, K. Krause, W. Klapper, K. Heidorn, B. E. Castle, J. Janda, K. B. Marcu, and R. Parwaresch Expression of activation-induced cytidine deaminase in human B-cell non-Hodgkin lymphomas Blood, May 1, 2003; 101(9): 3574 - 3580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Lehmann, S. Glockner, W. Kleeberger, H. F. R. von Wasielewski, and H. Kreipe Detection of Gene Amplification in Archival Breast Cancer Specimens by Laser-Assisted Microdissection and Quantitative Real-Time Polymerase Chain Reaction Am. J. Pathol., June 1, 2000; 156(6): 1855 - 1864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-M. C. Dingemans, M. A. Witlox, R. A. L. M. Stallaert, P. van der Valk, P. E. Postmus, and G. Giaccone Expression of DNA Topoisomerase II{{alpha}} and Topoisomerase II{beta} Genes Predicts Survival and Response to Chemotherapy in Patients with Small Cell Lung Cancer Clin. Cancer Res., August 1, 1999; 5(8): 2048 - 2058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Rudolph, P. Alm, H.-J. Heidebrecht, H. Bolte, V. Ratjen, B. Baldetorp, M. Ferno, H. Olsson, and R. Parwaresch Immunologic Proliferation Marker Ki-S2 as Prognostic Indicator for Lymph Node-Negative Breast Cancer J Natl Cancer Inst, February 3, 1999; 91(3): 271 - 278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Grue, A. Grasser, M. Sehested, P. B. Jensen, A. Uhse, T. Straub, W. Ness, and F. Boege Essential Mitotic Functions of DNA Topoisomerase IIalpha Are Not Adopted by Topoisomerase IIbeta in Human H69 Cells J. Biol. Chem., December 11, 1998; 273(50): 33660 - 33666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.J. Heidebrecht, F. Buck, J. Steinmann, R. Sprenger, H.H. Wacker, and R. Parwaresch p100: A Novel Proliferation-Associated Nuclear Protein Specifically Restricted to Cell Cycle Phases S, G2 , and M Blood, July 1, 1997; 90(1): 226 - 233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. N. Meyer, E. Kjeldsen, T. Straub, B. R. Knudsen, I. D. Hickson, A. Kikuchi, H. Kreipe, and F. Boege Cell Cycle-coupled Relocation of Types I and II Topoisomerases and Modulation of Catalytic Enzyme Activities J. Cell Biol., February 24, 1997; 136(4): 775 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Kellner, H.-J. Heidebrecht, P. Rudolph, H. Biersack, F. Buck, T. Dakowski, H.-H. Wacker, M. Domanowski, A. Seidel, O. Westergaard, et al. Detection of Human Topoisomerase II{alpha} in Cell Lines and Tissues: Characterization of Five Novel Monoclonal Antibodies J. Histochem. Cytochem., February 1, 1997; 45(2): 251 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Casiano, G Landberg, R. Ochs, and E. Tan Autoantibodies to a novel cell cycle-regulated protein that accumulates in the nuclear matrix during S phase and is localized in the kinetochores and spindle midzone during mitosis J. Cell Sci., January 12, 1993; 106(4): 1045 - 1056. [Abstract] [PDF]
|
|
|
|
|
|
H. U. Barthelmes, P. Grue, S. Feineis, T. Straub, and F. Boege Active DNA Topoisomerase IIalpha Is a Component of the Salt-stable Centrosome Core J. Biol. Chem., December 1, 2000; 275(49): 38823 - 38830. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
