This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kradin, R. L. Articles by Schneeberger, E. E. Search for Related Content PubMed PubMed Citation Articles by Kradin, R. L. Articles by Schneeberger, E. E.

American Journal of Pathology, Vol 142, 811-819, Copyright © 1993 by American Society for Investigative Pathology

REGULAR ARTICLES

FcR+/- subsets of Ia+ pulmonary dendritic cells in the rat display differences in their abilities to provide accessory co-stimulation for naive (OX-22+) and sensitized (OX-22-) T cells

RL Kradin, W Xia, K McCarthy and EE Schneeberger
Department of Pathology, Massachusetts General Hospital, Boston 02114.

A substantial body of evidence indicates that the primary sensitization of naive T cells to inhaled antigens occurs in the regional lymph nodes, whereas secondary responses may be generated directly within lung tissue. Ia+ pulmonary dendritic cells are widely distributed within the rat lung where they can participate in the induction of the immune response to inhaled antigens. Recently, two subsets of Ia+ pulmonary dendritic cells have been distinguished based on their expression of Fc receptors (FcR), but little is known concerning their abilities to support the responses of naive or sensitized T cells. In order to address this question, pulmonary FcR+/- dendritic cells have been purified from enzymatic digests of Lewis rat lungs, based on their differential binding to heat-aggregated immunoglobulin. The FcR+/- dendritic cell subsets differed with respect to their light microscopic appearance and in their expression of non-specific esterase. Only the FcR+ subset was able to phagocytize latex beads and showed intracellular phagolysosomes by electron microscopy. Both of the FcR+/- subsets rapidly formed clusters with naive (OX-22+) and sensitized (OX- 22-) T cells. However, the clusters yielded by the FcR+ subset were substantially smaller, possibly reflecting their diminished surface membrane expression of the intercellular adhesion molecule-1. The FcR+/- subsets were capable of presenting soluble and particulate antigens to OX-22- T cells. FcR+ cells were less effective than FcR- cells in promoting the proliferative response of OX-22+ T cells to concanavalin A and in the primary mixed leukocyte reaction. We conclude that the FcR+/- pulmonary dendritic cells differ in their abilities to support the responses of naive and sensitized T lymphocytes. This observation may have significance for how primary and secondary pulmonary cell- mediated immune responses are generated.

This article has been cited by other articles:

				R. L. KRADIN, H.-W. LIU, N. van ROOIJEN, K. SPRINGER, L.-H. ZHAO, and C. P. LEARY Pulmonary Immunity to Listeria Is Enhanced by Elimination of Alveolar Macrophages Am. J. Respir. Crit. Care Med., June 1, 1999; 159(6): 1967 - 1974. [Abstract] [Full Text]

				L. Liu, M. Zhang, C. Jenkins, and G. G. MacPherson Dendritic Cell Heterogeneity In Vivo: Two Functionally Different Dendritic Cell Populations in Rat Intestinal Lymph Can Be Distinguished by CD4 Expression J. Immunol., August 1, 1998; 161(3): 1146 - 1155. [Abstract] [Full Text] [PDF]