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American Journal of Pathology, Vol 142, 821-829, Copyright © 1993 by American Society for Investigative Pathology

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Epitope specificity of anti-gp330 autoantibodies determines the development of proteinuria in active Heymann nephritis

EH Van Leer, P Ronco, P Verroust, AM van der Wal, PJ Hoedemaeker and E De Heer
Department of Pathology, University of Leiden, The Netherlands.

In active Heymann nephritis, an experimental autoimmune disease in the rat, gp330 is regarded as the main antigenic target. Immunization with detergent-solubilized renal tubular epithelium (RTE-DOC) has been shown to be less nephritogenic than immunization with crude RTE. In this study immunization with either crude RTE or affinity-purified gp330 did, but immunization with RTE-DOC did not induce proteinuria. Both a possible aberrant subclass distribution of anti-gp330 autoantibodies and the involvement of additional nephritogenic autoantigens such as DPP IV (gp90) or laminin could be excluded. Circulating anti-gp330 autoantibody titers were significantly higher in RTE-DOC-immunized rats than in RTE-immunized animals. In contrast, significantly more antibodies were shown to bind in the glomeruli in the latter group. The time of onset of abnormal proteinuria was shown to be related to the recognition of a particular V8 protease-induced 250 kD fragment of gp330 in Western blots. This study shows that a particular fragment- specific subset of autoantibodies against gp330 is involved in the glomerular damage in Heymann nephritis.

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