help button home button Am J Pathol Epitomics Buy 2 Antibodies Get 1 Free Special Offer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Greve, G.
Right arrow Articles by Saetersdal, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Greve, G.
Right arrow Articles by Saetersdal, T.

American Journal of Pathology, Vol 142, 1279-1289, Copyright © 1993 by American Society for Investigative Pathology

REGULAR ARTICLES

Reversibility of mild to moderate ischemic injuries in the isolated rat heart. A characterization by 31P-NMR and by physiological and ultrastructural indices

G Greve, OE Bakoy, T Holten, P Jynge and T Saetersdal
Department of Anatomy, University of Bergen, Norway.

We have studied cardiac function, metabolism, and ultrastructure during reperfusion after global ischemia of short duration (6 and 12 minutes) in isolated rat hearts. Our aim was to obtain more detailed information on the reversibility of changes following presumedly mild and moderate ischemic injuries by use of multiple time-based indices. In a modified Langendorff perfusion system, hearts were subjected to 24 minutes of control perfusion and 6 or 12 minutes of ischemia followed by 1.5 or 24 minutes of reperfusion. During the experiments we monitored left ventricular developed pressure (LVDP), heart rate, and coronary flow rate, and intracellular phosphocreatine (PCr), inorganic phosphate (P(i)), pH, and ATP by 31P nuclear magnetic resonance spectroscopy. The number of cells with sarcolemmal and nuclear injuries was counted. Our main findings during 24 minutes of reperfusion following 6 and 12 minutes of ischemia were 80% versus 53% recovery of LVDP at the end of reperfusion, an increased PCr, 80% versus 65% recovery of ATP, and a rapid versus slower recovery of pH. Ultrastructural examination revealed sarcolemmal and unclear abnormalities at the end of ischemia, these alterations being fully (rapidly versus more slowly) reversible during reperfusion. According to these findings, there was a dissociation between an essentially normal ultrastructure, and a depressed recovery of LVDP, reduced ATP, and an overshoot of PCr upon 24 minutes of reperfusion after 12 minutes of ischemia. This may indicate a postischemic dysfunction closely related to stunning.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the American Society for Investigative Pathology.