This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Falini, B. Articles by Cavaliere, A. Search for Related Content PubMed PubMed Citation Articles by Falini, B. Articles by Cavaliere, A.

American Journal of Pathology, Vol 142, 1359-1372, Copyright © 1993 by American Society for Investigative Pathology

REGULAR ARTICLES

PG-M1: a new monoclonal antibody directed against a fixative-resistant epitope on the macrophage-restricted form of the CD68 molecule

B Falini, L Flenghi, S Pileri, M Gambacorta, B Bigerna, H Durkop, F Eitelbach, J Thiele, R Pacini and A Cavaliere
Institute of Hematology, Perugia University, Italy.

A new anti-macrophage monoclonal antibody (PG-M1) was produced by immunizing BALB/c mice with fresh spleen cells from a patient with Gaucher's disease. PG-M1 reacts strongly with a fixative-resistant epitope of an intracytoplasmic molecule, selectively expressed by virtually all macrophages of the human body. Although attempts to immunoprecipitate the molecule recognized by PG-M1 have failed so far, the reactivity of the antibody with COS-1 and WOP cells transfected with a human complementary DNA clone encoding for the CD68 antigen suggests that PG-M1 is a new member of the CD68 cluster. However, unlike other CD68 antibodies (KP1, EBM11, etc.), which react with both macrophages and myeloid cells, PG-M1 detects a fixative-resistant epitope on the macrophage-restricted form of the CD68 antigen. In 957 routinely fixed, paraffin-embedded samples, PG-M1 showed a more restricted reactivity with elements of the monocyte/macrophage lineage than the previously described monoclonal antibodies MAC-387 (anti- calgranulins), KP1 (CD68) and Ki-M1P. Among hematological malignancies, PG-M1 only labels acute leukemias of M4 and M5 type and rare examples of malignant histiocytosis/true histiocytic sarcoma. In contrast, acute leukemias of the M1, M2, M3, M6, M7, and L1-L3 types, non-Hodgkin's lymphomas, and Hodgkin and Reed-Sternberg cells of Hodgkin's disease are consistently PG-M1-negative. In the daily diagnostic practice, PG- M1 seems to be particularly valuable for the diagnosis of myelomonocytic or monocytic leukemia and neoplasms of true histiocytic origin in routine paraffin sections.

This article has been cited by other articles:

				L. Lyck, I. Dalmau, J. Chemnitz, B. Finsen, and H. D. Schroder Immunohistochemical Markers for Quantitative Studies of Neurons and Glia in Human Neocortex J. Histochem. Cytochem., March 1, 2008; 56(3): 201 - 221. [Abstract] [Full Text] [PDF]

				W. Maat, L. V. Ly, E. S. Jordanova, D. de Wolff-Rouendaal, N. E. Schalij-Delfos, and M. J. Jager Monosomy of Chromosome 3 and an Inflammatory Phenotype Occur Together in Uveal Melanoma Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 505 - 510. [Abstract] [Full Text] [PDF]

				M. Pierer, F. Brentano, J. Rethage, U. Wagner, H. Hantzschel, R. E. Gay, S. Gay, and D. Kyburz The TNF superfamily member LIGHT contributes to survival and activation of synovial fibroblasts in rheumatoid arthritis Rheumatology, July 1, 2007; 46(7): 1063 - 1070. [Abstract] [Full Text] [PDF]

				A. C. B. Molteno, A. J. Suter, M. Fenwick, T. H. Bevin, and A. G. Dempster Otago Glaucoma Surgery Outcome Study: Cytology and Immunohistochemical Staining of Bleb Capsules around Molteno Implants Invest. Ophthalmol. Vis. Sci., May 1, 2006; 47(5): 1975 - 1981. [Abstract] [Full Text] [PDF]

				T. J. Welsh, R. H. Green, D. Richardson, D. A. Waller, K. J. O'Byrne, and P. Bradding Macrophage and Mast-Cell Invasion of Tumor Cell Islets Confers a Marked Survival Advantage in Non-Small-Cell Lung Cancer J. Clin. Oncol., December 10, 2005; 23(35): 8959 - 8967. [Abstract] [Full Text] [PDF]

				M. Saruta, K. Takahashi, T. Suzuki, A. Torii, M. Kawakami, and H. Sasano Urocortin 1 in Colonic Mucosa in Patients with Ulcerative Colitis J. Clin. Endocrinol. Metab., November 1, 2004; 89(11): 5352 - 5361. [Abstract] [Full Text] [PDF]

				E. Tiacci, S. Pileri, A. Orleth, R. Pacini, A. Tabarrini, F. Frenguelli, A. Liso, D. Diverio, F. Lo-Coco, and B. Falini PAX5 Expression in Acute Leukemias: Higher B-Lineage Specificity Than CD79a and Selective Association with t(8;21)-Acute Myelogenous Leukemia Cancer Res., October 15, 2004; 64(20): 7399 - 7404. [Abstract] [Full Text] [PDF]

				E Kunisch, R Fuhrmann, A Roth, R Winter, W Lungershausen, and R W Kinne Macrophage specificity of three anti-CD68 monoclonal antibodies (KP1, EBM11, and PGM1) widely used for immunohistochemistry and flow cytometry Ann Rheum Dis, July 1, 2004; 63(7): 774 - 784. [Abstract] [Full Text] [PDF]

				K. Basso, A. Liso, E. Tiacci, R. Benedetti, A. Pulsoni, R. Foa, F. Di Raimondo, A. Ambrosetti, A. Califano, U. Klein, et al. Gene Expression Profiling of Hairy Cell Leukemia Reveals a Phenotype Related to Memory B Cells with Altered Expression of Chemokine and Adhesion Receptors J. Exp. Med., January 5, 2004; 199(1): 59 - 68. [Abstract] [Full Text] [PDF]

				B. V. Offersen, B. S. Nielsen, G. Hoyer-Hansen, F. Rank, S. Hamilton-Dutoit, J. Overgaard, and P. A. Andreasen The Myofibroblast Is the Predominant Plasminogen Activator Inhibitor-1-Expressing Cell Type in Human Breast Carcinomas Am. J. Pathol., November 1, 2003; 163(5): 1887 - 1899. [Abstract] [Full Text] [PDF]

				S. A. Pileri, F. Facchetti, S. Ascani, E. Sabattini, S. Poggi, M. Piccioli, D. Rondelli, F. Vergoni, P. L. Zinzani, P. P. Piccaluga, et al. Myeloperoxidase Expression by Histiocytes in Kikuchi's and Kikuchi-Like Lymphadenopathy Am. J. Pathol., September 1, 2001; 159(3): 915 - 924. [Abstract] [Full Text] [PDF]

				H. M. Kvasnicka, J. Thiele, A. Schmitt-Graeff, V. Diehl, R. Zankovich, N. Niederle, L.-D. Leder, and H. E. Schaefer Bone Marrow Features Improve Prognostic Efficiency in Multivariate Risk Classification of Chronic-Phase Ph1+ Chronic Myelogenous Leukemia: A Multicenter Trial J. Clin. Oncol., June 15, 2001; 19(12): 2994 - 3009. [Abstract] [Full Text] [PDF]

				T. Mäkitie, P. Summanen, A. Tarkkanen, and T. Kivelä Tumor-Infiltrating Macrophages (CD68+ Cells) and Prognosis in Malignant Uveal Melanoma Invest. Ophthalmol. Vis. Sci., June 1, 2001; 42(7): 1414 - 1421. [Abstract] [Full Text]

				L.L. Nikitenko, I.Z. MacKenzie, M.C.P. Rees, and R. Bicknell Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium Mol. Hum. Reprod., September 1, 2000; 6(9): 811 - 819. [Abstract] [Full Text] [PDF]

				K. L. Talks, H. Turley, K. C. Gatter, P. H. Maxwell, C. W. Pugh, P. J. Ratcliffe, and A. L. Harris The Expression and Distribution of the Hypoxia-Inducible Factors HIF-1{alpha} and HIF-2{alpha} in Normal Human Tissues, Cancers, and Tumor-Associated Macrophages Am. J. Pathol., August 1, 2000; 157(2): 411 - 421. [Abstract] [Full Text] [PDF]

				B. Vergier, A. de Muret, M. Beylot-Barry, L. Vaillant, D. Ekouevi, G. Chene, A. Carlotti, N. Franck, P. Dechelotte, P. Souteyrand, et al. Transformation of mycosis fungoides: clinicopathological and prognostic features of 45 cases Blood, April 1, 2000; 95(7): 2212 - 2218. [Abstract] [Full Text] [PDF]

				B. Falini, M. Fizzotti, A. Pucciarini, B. Bigerna, T. Marafioti, M. Gambacorta, R. Pacini, C. Alunni, L. Natali-Tanci, B. Ugolini, et al. A monoclonal antibody (MUM1p) detects expression of the MUM1/IRF4 protein in a subset of germinal center B cells, plasma cells, and activated T cells Blood, March 15, 2000; 95(6): 2084 - 2092. [Abstract] [Full Text] [PDF]

				J. Yamate, H. Yoshida, Y. Tsukamoto, M. Ide, M. Kuwamura, F. Ohashi, T. Miyamoto, T. Kotani, S. Sakuma, and M. Takeya Distribution of Cells Immunopositive for AM-3K, a Novel Monoclonal Antibody Recognizing Human Macrophages, in Normal and Diseased Tissues of Dogs, Cats, Horses, Cattle, Pigs, and Rabbits Vet. Pathol., March 1, 2000; 37(2): 168 - 176. [Abstract] [Full Text] [PDF]

				B. Falini, S. Pileri, P. L. Zinzani, A. Carbone, V. Zagonel, C. Wolf-Peeters, G. Verhoef, F. Menestrina, G. Todeschini, M. Paulli, et al. ALK+ Lymphoma: Clinico-Pathological Findings and Outcome Blood, April 15, 1999; 93(8): 2697 - 2706. [Abstract] [Full Text] [PDF]

				K. L. HUDKINS, C. M. GIACHELLI, YAN CUI, W. G. COUSER, R. J. JOHNSON, and C. E. ALPERS Osteopontin Expression in Fetal and Mature Human Kidney J. Am. Soc. Nephrol., March 1, 1999; 10(3): 444 - 457. [Abstract] [Full Text]

				M. Christgau, R. G. Caffesse, J. R. Newland, G. Schmalz, and R. N. D'Souza Characterization of Immunocompetent Cells in the Diseased Canine Periodontium J. Histochem. Cytochem., December 1, 1998; 46(12): 1443 - 1454. [Abstract] [Full Text]

				A. Fiumara, A. Belfiore, G. Russo, E. Salomone, G. M. Santonocito, O. Ippolito, R. Vigneri, and P. Gangemi In Situ Evidence of Neoplastic Cell Phagocytosis by Macrophages in Papillary Thyroid Cancer J. Clin. Endocrinol. Metab., May 1, 1997; 82(5): 1615 - 1620. [Abstract] [Full Text] [PDF]