This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Scarpa, A. Articles by Hirohashi, S. Search for Related Content PubMed PubMed Citation Articles by Scarpa, A. Articles by Hirohashi, S.

American Journal of Pathology, Vol 142, 1534-1543, Copyright © 1993 by American Society for Investigative Pathology

REGULAR ARTICLES

Pancreatic adenocarcinomas frequently show p53 gene mutations

A Scarpa, P Capelli, K Mukai, G Zamboni, T Oda, C Iacono and S Hirohashi
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.

Thirty-four pancreatic adenocarcinomas were studied for the presence of p53 gene mutations by the single-strand conformation polymorphism method and by direct sequencing of PCR-amplified fragments. p53 protein expression was immunohistochemically evaluated using monoclonal PAb1801 and polyclonal CM1 antibodies. Mutations were detected in 14 cases. The transitions were six G to A and two A to G; the transversions were one C to G and two A to C; the remaining three were frameshift mutations. Immunostaining results were identical with both antibodies. Nuclear immunohistochemical p53-positive cells were found in nine p53 mutated cases and in 12 cases in which no mutation was detected. In most of these latter cases only a minority of cancer cells showed immunohistochemical positivity. Twenty-nine cases, including all p53 mutated cancers, were known to contain codon 12 Ki-ras gene mutations. Also in the light of the demonstrated cooperation of ras and p53 gene alterations in the transformation of cultured cells, our data suggest that p53 mutation is one of the genetic defects that may have a role in the pathogenesis of a proportion of pancreatic cancers.

This article has been cited by other articles:

				T. B. Deramaudt, M. Takaoka, R. Upadhyay, M. J. Bowser, J. Porter, A. Lee, B. Rhoades, C. N. Johnstone, R. Weissleder, S. R. Hingorani, et al. N-Cadherin and Keratinocyte Growth Factor Receptor Mediate the Functional Interplay between Ki-RASG12V and p53V143A in Promoting Pancreatic Cell Migration, Invasion, and Tissue Architecture Disruption. Mol. Cell. Biol., June 1, 2006; 26(11): 4185 - 4200. [Abstract] [Full Text] [PDF]

				Y. Koga, Y. Kitajima, A. Miyoshi, K. Sato, K. Kitahara, H. Soejima, and K. Miyazaki Tumor Progression Through Epigenetic Gene Silencing of O6-Methylguanine-DNA Methyltransferase in Human Biliary Tract Cancers Ann. Surg. Oncol., May 1, 2005; 12(5): 354 - 363. [Abstract] [Full Text] [PDF]

				S. Yamamoto, Y. Tomita, Y. Hoshida, H. Nagano, K. Dono, K. Umeshita, M. Sakon, O. Ishikawa, H. Ohigashi, S. Nakamori, et al. Increased Expression of Valosin-Containing Protein (p97) is Associated With Lymph Node Metastasis and Prognosis of Pancreatic Ductal Adenocarcinoma Ann. Surg. Oncol., February 1, 2004; 11(2): 165 - 172. [Abstract] [Full Text] [PDF]

				F. H. Brembeck, F. S. Schreiber, T. B. Deramaudt, L. Craig, B. Rhoades, G. Swain, P. Grippo, D. A. Stoffers, D. G. Silberg, and A. K. Rustgi The Mutant K-ras Oncogene Causes Pancreatic Periductal Lymphocytic Infiltration and Gastric Mucous Neck Cell Hyperplasia in Transgenic Mice Cancer Res., May 1, 2003; 63(9): 2005 - 2009. [Abstract] [Full Text] [PDF]

				G. Esposito, S. Pucciarelli, R. Alaggio, L. Giacomelli, E. Marchiori, G. A. Iaderosa, M. L. Friso, P. Toppan, L. Chieco-Bianchi, and M. Lise p27kip1 Expression Is Associated With Tumor Response to Preoperative Chemoradiotherapy in Rectal Cancer Ann. Surg. Oncol., May 1, 2001; 8(4): 311 - 318. [Abstract] [Full Text] [PDF]

				P. S. Moore, G. Zamboni, A. Brighenti, D. Lissandrini, D. Antonello, P. Capelli, G. Rigaud, M. Falconi, and A. Scarpa Molecular Characterization of Pancreatic Serous Microcystic Adenomas : Evidence for a Tumor Suppressor Gene on Chromosome 10q Am. J. Pathol., January 1, 2001; 158(1): 317 - 321. [Abstract] [Full Text] [PDF]

				L. Zhang, D. L. Weddle, P. E. Thomas, B. Zheng, A. Castonguay, H. M. Schuller, and M. S. Miller Low Levels of Expression of Cytochromes P-450 in Normal and Cancerous Fetal Pancreatic Tissues of Hamsters Treated with NNK and/or Ethanol Toxicol. Sci., August 1, 2000; 56(2): 313 - 323. [Abstract] [Full Text] [PDF]

				M. Yamano, H. Fujii, T. Takagaki, N. Kadowaki, H. Watanabe, and T. Shirai Genetic Progression and Divergence in Pancreatic Carcinoma Am. J. Pathol., June 1, 2000; 156(6): 2123 - 2133. [Abstract] [Full Text] [PDF]

				J. Okami, H. Yamamoto, Y. Fujiwara, M. Tsujie, M. Kondo, S. Noura, S. Oshima, H. Nagano, K. Dono, K. Umeshita, et al. Overexpression of Cyclooxygenase-2 in Carcinoma of the Pancreas Clin. Cancer Res., August 1, 1999; 5(8): 2018 - 2024. [Abstract] [Full Text] [PDF]

				M. Kusumoto, T. Ogawa, K. Mizumoto, H. Ueno, H. Niiyama, N. Sato, M. Nakamura, and M. Tanaka Adenovirus-mediated p53 Gene Transduction Inhibits Telomerase Activity Independent of Its Effects on Cell Cycle Arrest and Apoptosis in Human Pancreatic Cancer Cells Clin. Cancer Res., August 1, 1999; 5(8): 2140 - 2147. [Abstract] [Full Text] [PDF]

				A. SCARPA and G. ZAMBONI Genomic Anomalies in Pancreatic Tumors Other Than Common Adenocarcinoma Ann. N.Y. Acad. Sci., June 30, 1999; 880(1): 179 - 190. [Abstract] [Full Text] [PDF]

				R. M. SCHMID, G. KLOPPEL, G. ADLER, and M. WAGNER Acinar-Ductal-Carcinoma Sequence in Transforming Growth Factor-{alpha} Transgenic Mice Ann. N.Y. Acad. Sci., June 30, 1999; 880(1): 219 - 230. [Abstract] [Full Text] [PDF]

				C. Sorio, A. Baron, S. Orlandini, G. Zamboni, P. Pederzoli, K. Huebner, and A. Scarpa The FHIT Gene Is Expressed in Pancreatic Ductular Cells and Is Altered in Pancreatic Cancers Cancer Res., March 1, 1999; 59(6): 1308 - 1314. [Abstract] [Full Text] [PDF]