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American Journal of Pathology, Vol 142, 1695-1700, Copyright © 1993 by American Society for Investigative Pathology

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Two types of putative preneoplastic lesions identified by hexosaminidase activity in whole-mounts of colons from F344 rats treated with carcinogen

TP Pretlow, MA O'Riordan, KM Spancake and TG Pretlow
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.

Previous studies identified as putative preneoplastic lesions 1) enzyme- altered foci in sections of methacrylate-embedded colon and 2) aberrant crypts in methylene blue-stained unembedded (whole-mount) colon and established that aberrant crypts embedded in methacrylate had enzyme alterations. We have now studied histochemically demonstrable hexosaminidase activity in unembedded or whole-mount preparations of colons from carcinogen-treated rats. These preparations have revealed two populations of crypts that are enzyme-altered: those that are morphologically altered or aberrant and those that are morphologically normal. Both populations can be quantified rigorously in less than an hour with whole-mount preparations reacted for hexosaminidase. The demonstration of phenotypic characteristics with histochemical techniques in whole-mount preparations should have wide applicability to functional studies in many normal and diseased tissues.

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