This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arai, T. Articles by Sugawara, I. Search for Related Content PubMed PubMed Citation Articles by Arai, T. Articles by Sugawara, I.

American Journal of Pathology, Vol 142, 1938-1944, Copyright © 1993 by American Society for Investigative Pathology

REGULAR ARTICLES

Reduced nm 23-H1 messenger RNA expression in metastatic lymph nodes from patients with papillary carcinoma of the thyroid

T Arai, M Watanabe, M Onodera, T Yamashita, A Masunaga, S Itoyama, K Itoh and I Sugawara
Department of Pathology, Saitama Medical Center, Saitama Medical School, Japan.

The nm 23 gene product, which possesses nucleoside diphosphate kinase activity, is a possible mediator of cancer cell invasion and metastasis. It has been divided into two distinct gene products, nm23- H1 and nm23-H2. We have developed a method for detecting nm 23-H1 and nm 23-H2 RNA using the polymerase chain reaction, based on the amplification of complementary DNA copies of nm 23-H1 and nm 23-H2 RNA. Using this method, the nm 23-H1 and nm 23-H2 messenger (m)RNA levels in 35 thyroid papillary carcinomas, 11 metastatic lymph nodes from patients with thyroid papillary carcinomas, five thyroid follicular adenomas, and three normal thyroid tissue samples were studied. Both nm 23-H1 and nm 23-H2 mRNA were expressed ubiquitously in normal and malignant thyroid tissues. However, in metastatic lymph nodes no (3 of 11) or weak expression (8 of 11) of nm 23-H1 mRNA was observed, the extent of which was inversely proportional to the degree of cancer cell occupancy, whereas nm 23-H2 mRNA was expressed and the levels were similar to those in other tissue tested. These results show that nm 23- H1 only may play a role in cancer cell invasion and metastasis although the exact mechanisms involved have yet to be elucidated.

This article has been cited by other articles:

				N. Stathatos, I. Bourdeau, A. V. Espinosa, M. Saji, V. V. Vasko, K. D. Burman, C. A. Stratakis, and M. D. Ringel KiSS-1/G Protein-Coupled Receptor 54 Metastasis Suppressor Pathway Increases Myocyte-Enriched Calcineurin Interacting Protein 1 Expression and Chronically Inhibits Calcineurin Activity J. Clin. Endocrinol. Metab., September 1, 2005; 90(9): 5432 - 5440. [Abstract] [Full Text] [PDF]

				C. Zafon, G. Obiols, J. CastellvI, N. Tallada, P. Galofre, E. Gemar, J. Mesa, and R. Simo nm23-H1 Immunoreactivity as a Prognostic Factor in Differentiated Thyroid Carcinoma J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3975 - 3980. [Abstract] [Full Text] [PDF]

				M. Tomita, T. Ayabe, Y. Matsuzaki, and T. Onitsuka Expression of nm23-H1 gene product in mediastinal lymph nodes from lung cancer patients Eur. J. Cardiothorac. Surg., June 1, 2001; 19(6): 904 - 907. [Abstract] [Full Text] [PDF]

				S. Abe, H. Takizawa, I. Sugawara, and S. Kudoh Diesel Exhaust (DE)-Induced Cytokine Expression in Human Bronchial Epithelial Cells . A Study with a New Cell Exposure System to Freshly Generated DE In Vitro Am. J. Respir. Cell Mol. Biol., March 1, 2000; 22(3): 296 - 303. [Abstract] [Full Text]