This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Villaschi, S. Articles by Nicosia, R. F. Search for Related Content PubMed PubMed Citation Articles by Villaschi, S. Articles by Nicosia, R. F.

American Journal of Pathology, Vol 143, 181-190, Copyright © 1993 by American Society for Investigative Pathology

REGULAR ARTICLES

Angiogenic role of endogenous basic fibroblast growth factor released by rat aorta after injury

S Villaschi and RF Nicosia
Department of Pathology, Medical College of Pennsylvania, Philadelphia 19129.

The autocrine role of basic fibroblast growth factor (bFGF) in angiogenesis was studied in the rat aortic ring-collagen gel model using serum-free culture conditions. Immunohistochemical staining of the rat aorta showed bFGF in the cytoplasm of endothelial and smooth muscle cells. Aortic rings mechanically injured during the dissection procedure released bFGF, which was demonstrated in the conditioned medium by slot and Western blot analysis. bFGF-containing aorta- conditioned medium and purified bFGF increased both the number and length of microvessels sprouting from the explants. This effect was particularly evident during the second week of culture, when the release of endogenous bFGF was minimal. Neutralizing anti-bFGF antibodies induced a 40% reduction of angiogenesis. Regression of microvessels, which regularly occurred toward the end of the second week, was prevented by purified bFGF. These data support the idea that bFGF released by vascular cells plays an important role in the autoregulation of angiogenesis after injury.

This article has been cited by other articles:

				M. Komori, Y. Tomizawa, K. Takada, and M. Ozaki A Single Local Application of Recombinant Human Basic Fibroblast Growth Factor Accelerates Initial Angiogenesis During Wound Healing in Rabbit Ear Chamber Anesth. Analg., March 1, 2005; 100(3): 830 - 834. [Abstract] [Full Text] [PDF]

				T. Licht, L. Tsirulnikov, H. Reuveni, T. Yarnitzky, and S. A. Ben-Sasson Induction of pro-angiogenic signaling by a synthetic peptide derived from the second intracellular loop of S1P3 (EDG3) Blood, September 15, 2003; 102(6): 2099 - 2107. [Abstract] [Full Text] [PDF]

				T. Akimoto and M. R. Hammerman Microvessel formation from mouse aorta is stimulated in vitro by secreted VEGF and extracts from metanephroi Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1625 - C1632. [Abstract] [Full Text] [PDF]

				W.-H. Zhu, A. MacIntyre, and R. F. Nicosia Regulation of Angiogenesis by Vascular Endothelial Growth Factor and Angiopoietin-1 in the Rat Aorta Model : Distinct Temporal Patterns of Intracellular Signaling Correlate with Induction of Angiogenic Sprouting Am. J. Pathol., September 1, 2002; 161(3): 823 - 830. [Abstract] [Full Text] [PDF]

				T. Gloe, H. Y. Sohn, G. A. Meininger, and U. Pohl Shear Stress-induced Release of Basic Fibroblast Growth Factor from Endothelial Cells Is Mediated by Matrix Interaction via Integrin alpha Vbeta 3 J. Biol. Chem., June 21, 2002; 277(26): 23453 - 23458. [Abstract] [Full Text] [PDF]

				D. Corseaux, T. Meurice, I. Six, L. Rugeri, M. D. Ezekowitz, P. Rouvier, R. Bordet, C. Bauters, and B. Jude Basic Fibroblast Growth Factor Increases Tissue Factor Expression in Circulating Monocytes and in Vascular Wall Circulation, April 25, 2000; 101(16): 2000 - 2006. [Abstract] [Full Text] [PDF]

				U. R. Pendurthi, L. V. M. Rao, J. T. Williams, and S. Idell Regulation of Tissue Factor Pathway Inhibitor Expression in Smooth Muscle Cells Blood, July 15, 1999; 94(2): 579 - 586. [Abstract] [Full Text] [PDF]

				S. Liekens, D. Leali, J. Neyts, R. Esnouf, M. Rusnati, P. Dell'Era, P. C. Maudgal, E. De Clercq, and M. Presta Modulation of Fibroblast Growth Factor-2 Receptor Binding, Signaling, and Mitogenic Activity by Heparin-Mimicking Polysulfonated Compounds Mol. Pharmacol., July 1, 1999; 56(1): 204 - 213. [Abstract] [Full Text]

				N Ilan, S Mahooti, and J. Madri Distinct signal transduction pathways are utilized during the tube formation and survival phases of in vitro angiogenesis J. Cell Sci., June 14, 1999; 111(24): 3621 - 3631. [Abstract] [PDF]

				K. J. Scheidegger, J. Du, and P. Delafontaine Distinct and Common Pathways in the Regulation of Insulin-like Growth Factor-1 Receptor Gene Expression by Angiotensin II and Basic Fibroblast Growth Factor J. Biol. Chem., February 5, 1999; 274(6): 3522 - 3530. [Abstract] [Full Text] [PDF]

				T Korff and H. Augustin Tensional forces in fibrillar extracellular matrices control directional capillary sprouting J. Cell Sci., January 10, 1999; 112(19): 3249 - 3258. [Abstract] [PDF]

				T. Korff and H. G. Augustin Integration of Endothelial Cells in Multicellular Spheroids Prevents Apoptosis and Induces Differentiation J. Cell Biol., November 30, 1998; 143(5): 1341 - 1352. [Abstract] [Full Text] [PDF]

				S. Kim-Schulze, W. L. Lowe Jr, and H. W. Schnaper Estrogen Stimulates Delayed Mitogen-Activated Protein Kinase Activity in Human Endothelial Cells via an Autocrine Loop That Involves Basic Fibroblast Growth Factor Circulation, August 4, 1998; 98(5): 413 - 421. [Abstract] [Full Text] [PDF]

				R. L. Panek, G. H. Lu, T. K. Dahring, B. L. Batley, C. Connolly, J. M. Hamby, and K. J. Brown In Vitro Biological Characterization and Antiangiogenic Effects of PD 166866, a Selective Inhibitor of the FGF-1 Receptor Tyrosine Kinase J. Pharmacol. Exp. Ther., July 1, 1998; 286(1): 569 - 577. [Abstract] [Full Text]

				A. Sahni, T. Odrljin, and C. W. Francis Binding of Basic Fibroblast Growth Factor to Fibrinogen and Fibrin J. Biol. Chem., March 27, 1998; 273(13): 7554 - 7559. [Abstract] [Full Text] [PDF]

				R. D. Kenagy, C. E. Hart, W. G. Stetler-Stevenson, and A. W. Clowes Primate Smooth Muscle Cell Migration From Aortic Explants Is Mediated by Endogenous Platelet-Derived Growth Factor and Basic Fibroblast Growth Factor Acting Through Matrix Metalloproteinases 2 and 9 Circulation, November 18, 1997; 96(10): 3555 - 3560. [Abstract] [Full Text]

				C.-H. Chen, J. Cartwright Jr, Z. Li, S. Lou, H. H. Nguyen, A. M. Gotto Jr, and P. D. Henry Inhibitory Effects of Hypercholesterolemia and Ox-LDL on Angiogenesis-like Endothelial Growth in Rabbit Aortic Explants : Essential Role of Basic Fibroblast Growth Factor Arterioscler. Thromb. Vasc. Biol., July 1, 1997; 17(7): 1303 - 1312. [Abstract] [Full Text]

				A. Bikfalvi, S. Klein, G. Pintucci, and D. B. Rifkin Biological Roles of Fibroblast Growth Factor-2 Endocr. Rev., February 1, 1997; 18(1): 26 - 45. [Abstract] [Full Text]