This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mackman, N. Articles by Loskutoff, D. J. Search for Related Content PubMed PubMed Citation Articles by Mackman, N. Articles by Loskutoff, D. J.

American Journal of Pathology, Vol 143, 76-84, Copyright © 1993 by American Society for Investigative Pathology

REGULAR ARTICLES

Murine tissue factor gene expression in vivo. Tissue and cell specificity and regulation by lipopolysaccharide

N Mackman, MS Sawdey, MR Keeton and DJ Loskutoff
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.

Regulation of tissue factor (TF) gene expression was studied in vivo employing a murine model system. In untreated mice, TF mRNA was detected in brain, lung, kidney, and heart by Northern blot analysis. After administration of lipopolysaccharide, steady-state levels of TF mRNA were unchanged in brain, decreased in heart, and increased in both kidney and lung. In the brain, Bergmann glia within the Purkinje cell layer of the cerebellum and neuroglia within the cerebral cortex expressed TF mRNA by in situ hybridization. Epidermal cells of the skin and tongue also expressed TF mRNA. At present, we have not identified the cell type(s) in the kidney and lung responsible for increased TF gene expression. These results demonstrate tissue- and cell-specific TF gene expression in vivo. Lipopolysaccharide-mediated increases in TF expression in the kidney and lung may promote fibrin deposition in these organs during Gram-negative sepsis.

This article has been cited by other articles:

				N. Mackman, R. E. Tilley, and N. S. Key Role of the Extrinsic Pathway of Blood Coagulation in Hemostasis and Thrombosis Arterioscler. Thromb. Vasc. Biol., August 1, 2007; 27(8): 1687 - 1693. [Abstract] [Full Text] [PDF]

				B. Szotowski, P. Goldin-Lang, S. Antoniak, V. Y. Bogdanov, D. Pathirana, M. Pauschinger, A. Dorner, U. Kuehl, S. Coupland, Y. Nemerson, et al. Alterations in myocardial tissue factor expression and cellular localization in dilated cardiomyopathy J. Am. Coll. Cardiol., April 5, 2005; 45(7): 1081 - 1089. [Abstract] [Full Text] [PDF]

				N. Mackman Role of Tissue Factor in Hemostasis, Thrombosis, and Vascular Development Arterioscler. Thromb. Vasc. Biol., June 1, 2004; 24(6): 1015 - 1022. [Abstract] [Full Text] [PDF]

				R. Pawlinski, B. Pedersen, B. Kehrle, W. C. Aird, R. D. Frank, M. Guha, and N. Mackman Regulation of tissue factor and inflammatory mediators by Egr-1 in a mouse endotoxemia model Blood, May 15, 2003; 101(10): 3940 - 3947. [Abstract] [Full Text] [PDF]

				F. Samad, M. Pandey, and D. J. Loskutoff Regulation of tissue factor gene expression in obesity Blood, December 1, 2001; 98(12): 3353 - 3358. [Abstract] [Full Text] [PDF]

				Y. H. Yang, P. Carmeliet, and J. A. Hamilton Tissue-Type Plasminogen Activator Deficiency Exacerbates Arthritis J. Immunol., July 15, 2001; 167(2): 1047 - 1052. [Abstract] [Full Text] [PDF]

				J. H. Erlich, E. M. Boyle, J. Labriola, J. C. Kovacich, R. A. Santucci, C. Fearns, E. N. Morgan, W. Yun, T. Luther, O. Kojikawa, et al. Inhibition of the Tissue Factor-Thrombin Pathway Limits Infarct Size after Myocardial Ischemia-Reperfusion Injury by Reducing Inflammation Am. J. Pathol., December 1, 2000; 157(6): 1849 - 1862. [Abstract] [Full Text] [PDF]

				J. Erlich, G. C. N. Parry, C. Fearns, M. Muller, P. Carmeliet, T. Luther, and N. Mackman Tissue factor is required for uterine hemostasis and maintenance of the placental labyrinth during gestation PNAS, July 6, 1999; 96(14): 8138 - 8143. [Abstract] [Full Text] [PDF]

				J. Erlich, C. Fearns, J. Mathison, R. J. Ulevitch, and N. Mackman Lipopolysaccharide Induction of Tissue Factor Expression in Rabbits Infect. Immun., May 1, 1999; 67(5): 2540 - 2546. [Abstract] [Full Text] [PDF]

				K. Yamamoto, V. de Waard, C. Fearns, and D. J. Loskutoff Tissue Distribution and Regulation of Murine von Willebrand Factor Gene Expression In Vivo Blood, October 15, 1998; 92(8): 2791 - 2801. [Abstract] [Full Text] [PDF]

				K. Yamamoto and D. J. Loskutoff Extrahepatic Expression and Regulation of Protein C in the Mouse Am. J. Pathol., August 1, 1998; 153(2): 547 - 555. [Abstract] [Full Text] [PDF]

				F. Samad, M. Pandey, and D. J. Loskutoff Tissue factor gene expression in the adipose tissues of obese mice PNAS, June 23, 1998; 95(13): 7591 - 7596. [Abstract] [Full Text] [PDF]

				S. E. Tsirka, T. H. Bugge, J. L. Degen, and S. Strickland Neuronal death in the central nervous system demonstrates a non-fibrin substrate for plasmin PNAS, September 2, 1997; 94(18): 9779 - 9781. [Abstract] [Full Text] [PDF]

				M.-Z. Cui, G. C. N. Parry, P. Oeth, H. Larson, M. Smith, R.-P. Huang, E. D. Adamson, and N. Mackman Transcriptional Regulation of the Tissue Factor Gene in Human Epithelial Cells Is Mediated by Sp1 and EGR-1 J. Biol. Chem., February 2, 1996; 271(5): 2731 - 2739. [Abstract] [Full Text] [PDF]

				A. Bierhaus, Y. Zhang, Y. Deng, N. Mackman, P. Quehenberger, M. Haase, T. Luther, M. Müller, H. Böhrer, J. Greten, et al. Mechanism of the Tumor Necrosis Factor alpha-mediated Induction of Endothelial Tissue Factor J. Biol. Chem., November 3, 1995; 270(44): 26419 - 26432. [Abstract] [Full Text] [PDF]