This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Charlotte, F. Articles by Zafrani, E. S. Search for Related Content PubMed PubMed Citation Articles by Charlotte, F. Articles by Zafrani, E. S.

American Journal of Pathology, Vol 144, 460-465, Copyright © 1994 by American Society for Investigative Pathology

REGULAR ARTICLES

Immunohistochemical detection of bcl-2 protein in normal and pathological human liver

F Charlotte, A L'Hermine, N Martin, Y Geleyn, M Nollet, P Gaulard and ES Zafrani
Service d'Anatomie et de Cytologie Pathologiques, Hopital Henri Mondor, Creteil, France.

The bcl-2 protein, which prolongs cell survival by blocking apoptosis, is expressed by progenitor cells in several self-renewing tissues and by tumoral cells in some extrahepatic neoplasms. Because the liver is a slow self-renewing tissue, an immunohistochemical study of the cellular distribution of the bcl-2 protein was performed in normal liver (12 cases), nontumoral hepatic lesions (33 cases), and benign or malignant liver tumors (46 cases). In normal liver, bcl-2 was expressed by bile ductules and small bile duct epithelium, but not by hepatocytes or large bile duct epithelium. In cirrhosis and focal nodular hyperplasia, epithelial cells of the ductular proliferation were bcl-2-positive. Eight of 11 cholangiocarcinomas stained positively for bcl-2, whereas all 15 hepatocellular carcinomas were bcl-2-negative. bcl-2 was also expressed in 6 of 14 metastatic adenocarcinomas. These findings suggest that the ductular cells and small bile duct epithelial cells might have a prolonged survival and might be hepatic progenitor cells. In addition, the bcl-2 protein appears to be a marker of cholangiocarcinoma but not of hepatocellular carcinoma and could help in distinguishing between these two primary liver tumors.

This article has been cited by other articles:

				L. Gramantieri, F. Fornari, M. Ferracin, A. Veronese, S. Sabbioni, G. A. Calin, G. L. Grazi, C. M. Croce, L. Bolondi, and M. Negrini MicroRNA-221 Targets Bmf in Hepatocellular Carcinoma and Correlates with Tumor Multifocality Clin. Cancer Res., August 15, 2009; 15(16): 5073 - 5081. [Abstract] [Full Text] [PDF]

				E Novo, F Marra, E Zamara, L Valfre di Bonzo, L Monitillo, S Cannito, I Petrai, A Mazzocca, A Bonacchi, R S M De Franco, et al. Overexpression of Bcl-2 by activated human hepatic stellate cells: resistance to apoptosis as a mechanism of progressive hepatic fibrogenesis in humans Gut, August 1, 2006; 55(8): 1174 - 1182. [Abstract] [Full Text] [PDF]

				A. Barrier, N. Olaya, F. Chiappini, F. Roser, O. Scatton, C. Artus, B. Franc, S. Dudoit, A. Flahault, B. Debuire, et al. Ischemic preconditioning modulates the expression of several genes, leading to the overproduction of IL-1Ra, iNOS, and Bcl-2 in a human model of liver ischemia-reperfusion FASEB J, October 1, 2005; 19(12): 1617 - 1626. [Abstract] [Full Text] [PDF]

				I. Martin-Burriel, N. O. Roome, O. Dorchies, and A. Prenez Histopathological and Molecular Changes During Apoptosis Produced by 7H-Dibenzo[c,g]-Carbazole in Mouse Liver Toxicol Pathol, February 1, 2004; 32(2): 202 - 211. [Abstract] [PDF]

				A C Okaro, D A Fennell, M Corbo, B R Davidson, and F E Cotter Pk11195, a mitochondrial benzodiazepine receptor antagonist, reduces apoptosis threshold in Bcl-XL and Mcl-1 expressing human cholangiocarcinoma cells Gut, October 1, 2002; 51(4): 556 - 561. [Abstract] [Full Text] [PDF]

				A C Okaro, A R Deery, R R Hutchins, and B R Davidson The expression of antiapoptotic proteins Bcl-2, Bcl-XL, and Mcl-1 in benign, dysplastic, and malignant biliary epithelium J. Clin. Pathol., December 1, 2001; 54(12): 927 - 932. [Abstract] [Full Text] [PDF]

				E. O. Souto, H. Miyoshi, R. N. Dubois, and G. J. Gores Kupffer cell-derived cyclooxygenase-2 regulates hepatocyte Bcl-2 expression in choledocho-venous fistula rats Am J Physiol Gastrointest Liver Physiol, May 1, 2001; 280(5): G805 - G811. [Abstract] [Full Text] [PDF]

				L. Fabris, M. Strazzabosco, H. A. Crosby, G. Ballardini, S. G. Hubscher, D. A. Kelly, J. M. Neuberger, A. J. Strain, and R. Joplin Characterization and Isolation of Ductular Cells Coexpressing Neural Cell Adhesion Molecule and Bcl-2 from Primary Cholangiopathies and Ductal Plate Malformations Am. J. Pathol., May 1, 2000; 156(5): 1599 - 1612. [Abstract] [Full Text] [PDF]

				T. Umino, E. Kusano, S. Muto, T. Akimoto, S. Yanagiba, S. Ono, M. Amemiya, Y. Ando, S. Homma, U. Ikeda, et al. AVP inhibits LPS- and IL-1beta -stimulated NO and cGMP via V1 receptor in cultured rat mesangial cells Am J Physiol Renal Physiol, March 1, 1999; 276(3): F433 - F441. [Abstract] [Full Text] [PDF]

				Y. Soini, P. Paakko, and V-P. Lehto Histopathological Evaluation of Apoptosis in Cancer Am. J. Pathol., October 1, 1998; 153(4): 1041 - 1053. [Full Text] [PDF]

				Proceedings of the British Toxicology Society Annual Congress University of Surrey, Guildford 19 - 22 April 1998 Human and Experimental Toxicology, September 1, 1998; 17(9): 469 - 534. [PDF]

				S. Eissa and L. S. Seada Quantitation of bcl-2 protein in bladder cancer tissue by enzyme immunoassay: comparison with Western blot and immunohistochemistry Clin. Chem., July 1, 1998; 44(7): 1423 - 1429. [Abstract] [Full Text] [PDF]