| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 144, 997-1007, Copyright © 1994 by American Society for Investigative Pathology
REGULAR ARTICLES |
L Mazzucchelli, C Hauser, K Zgraggen, H Wagner, M Hess, JA Laissue and C Mueller
Institute of Pathology, University of Bern, Switzerland.
Interleukin-8 (IL-8) is a potent cytokine for recruitment and activation of neutrophils. To visualize its distribution in the intestinal mucosa and to understand better its possible role in the induction and promotion of inflammatory bowel disease, expression of the IL-8 gene was analyzed in resected bowel segments of 14 patients with active Crohn's disease or ulcerative colitis. In situ hybridization with IL-8 anti-sense RNA probes revealed strong and specific signals in the histologically affected mucosa. The number of cells expressing IL-8 gene correlated with the histological grade of active inflammation. In accordance with the characteristic histological signs of active disease, IL-8-expressing cells were diffusely distributed over the entire affected mucosa in patients with ulcerative colitis, whereas in patients with Crohn's disease, IL-8-expressing cells showed a focal distribution pattern. Cells expressing IL-8 were mainly located at the base of ulcers, in inflammatory exudates on mucosal surfaces, in crypt abscesses, and at the border of fistulae. Analysis of semi-serial sections pointed to macrophages, neutrophils, and epithelial cells as possible sources of this cytokine in active inflammatory bowel disease. We consistently failed to detect IL-8 messenger RNA in the mucosa of uninvolved bowel segments and in normal- appearing control mucosa of patients with colon cancer. In contrast, tissue specimens from two patients with acute appendicitis displayed IL- 8-expressing cells in the mucosa. These results support the notion that IL-8 plays and important but nonspecific role in the pathogenesis of inflammatory bowel disease and that the production of IL-8 messenger RNA is restricted to areas with histological signs of inflammatory activity and mucosal destruction.
This article has been cited by other articles:
 |
|
 |
|
  P. Buanne, E. Di Carlo, L. Caputi, L. Brandolini, M. Mosca, F. Cattani, L. Pellegrini, L. Biordi, G. Coletti, C. Sorrentino, et al. Crucial pathophysiological role of CXCR2 in experimental ulcerative colitis in mice J. Leukoc. Biol., November 1, 2007; 82(5): 1239 - 1246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Danese, F Scaldaferri, S Vetrano, T Stefanelli, C Graziani, A Repici, R Ricci, G Straface, A Sgambato, A Malesci, et al. Critical role of the CD40 CD40-ligand pathway in regulating mucosal inflammation-driven angiogenesis in inflammatory bowel disease Gut, September 1, 2007; 56(9): 1248 - 1256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhao, M.-J. Kwon, S. Huang, J. Y. Lee, K. Fukase, N. Inohara, and D. H. Hwang Differential Modulation of Nods Signaling Pathways by Fatty Acids in Human Colonic Epithelial HCT116 Cells J. Biol. Chem., April 20, 2007; 282(16): 11618 - 11628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-H. Shim, T. Suzuki, S.-Y. Chang, S.-M. Park, P. J. Sansonetti, C. Sasakawa, and M.-N. Kweon New Animal Model of Shigellosis in the Guinea Pig: Its Usefulness for Protective Efficacy Studies J. Immunol., February 15, 2007; 178(4): 2476 - 2482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. T. Chiu, W. Y. Leung, M. P. Moyer, R. M. Strieter, and E. Rozengurt Protein kinase D2 mediates lysophosphatidic acid-induced interleukin 8 production in nontransformed human colonic epithelial cells through NF-{kappa}B Am J Physiol Cell Physiol, February 1, 2007; 292(2): C767 - C777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y Nishio, T Ando, O Maeda, K Ishiguro, O Watanabe, N Ohmiya, Y Niwa, K Kusugami, and H Goto Pit patterns in rectal mucosa assessed by magnifying colonoscope are predictive of relapse in patients with quiescent ulcerative colitis Gut, December 1, 2006; 55(12): 1768 - 1773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. I. Khan, Y. Motomura, H. Wang, R. T. El-Sharkawy, E. F. Verdu, M. Verma-Gandhu, B. J. Rollins, and S. M. Collins Critical role of MCP-1 in the pathogenesis of experimental colitis in the context of immune and enterochromaffin cells Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G803 - G811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Canny, E. Cario, A. Lennartsson, U. Gullberg, C. Brennan, O. Levy, and S. P. Colgan Functional and biochemical characterization of epithelial bactericidal/permeability-increasing protein Am J Physiol Gastrointest Liver Physiol, March 1, 2006; 290(3): G557 - G567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Mueller and A J Macpherson Layers of mutualism with commensal bacteria protect us from intestinal inflammation Gut, February 1, 2006; 55(2): 276 - 284. [Full Text] [PDF]
|
|
|
|
|
|
J. H. Kwon, A. C. Keates, P. M. Anton, M. Botero, J. D. Goldsmith, and C. P. Kelly Topical antisense oligonucleotide therapy against LIX, an enterocyte-expressed CXC chemokine, reduces murine colitis Am J Physiol Gastrointest Liver Physiol, December 1, 2005; 289(6): G1075 - G1083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Kucharzik, J T Hudson III, A Lugering, J A Abbas, M Bettini, J G Lake, M E Evans, T R Ziegler, D Merlin, J L Madara, et al. Acute induction of human IL-8 production by intestinal epithelium triggers neutrophil infiltration without mucosal injury Gut, November 1, 2005; 54(11): 1565 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Singer and P. J. Sansonetti IL-8 Is a Key Chemokine Regulating Neutrophil Recruitment in a New Mouse Model of Shigella-Induced Colitis J. Immunol., September 15, 2004; 173(6): 4197 - 4206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S J Connor, N Paraskevopoulos, R Newman, N Cuan, T Hampartzoumian, A R Lloyd, and M C Grimm CCR2 expressing CD4+ T lymphocytes are preferentially recruited to the ileum in Crohn's disease Gut, September 1, 2004; 53(9): 1287 - 1294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Alzoghaibi, S. W. Walsh, A. Willey, D. R. Yager, A. A. Fowler III, and M. F. Graham Linoleic acid induces interleukin-8 production by Crohn's human intestinal smooth muscle cells via arachidonic acid metabolites Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G528 - G537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Berkes, V K Viswanathan, S D Savkovic, and G Hecht Intestinal epithelial responses to enteric pathogens: effects on the tight junction barrier, ion transport, and inflammation Gut, March 1, 2003; 52(3): 439 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Heidemann, H. Ogawa, M. B. Dwinell, P. Rafiee, C. Maaser, H. R. Gockel, M. F. Otterson, D. M. Ota, N. Lugering, W. Domschke, et al. Angiogenic Effects of Interleukin 8 (CXCL8) in Human Intestinal Microvascular Endothelial Cells Are Mediated by CXCR2 J. Biol. Chem., February 28, 2003; 278(10): 8508 - 8515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. H. Nemeth, E. A. Deitch, C. Szabo, J. G. Mabley, P. Pacher, Z. Fekete, C. J. Hauser, and G. Hasko Na+/H+ exchanger blockade inhibits enterocyte inflammatory response and protects against colitis Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G122 - G132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. D. Mennechet, L. H. Kasper, N. Rachinel, W. Li, A. Vandewalle, and D. Buzoni-Gatel Lamina Propria CD4+ T Lymphocytes Synergize with Murine Intestinal Epithelial Cells to Enhance Proinflammatory Response Against an Intracellular Pathogen J. Immunol., March 15, 2002; 168(6): 2988 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Uguccioni, P. Gionchetti, D. F. Robbiani, F. Rizzello, S. Peruzzo, M. Campieri, and M. Baggiolini Increased Expression of IP-10, IL-8, MCP-1, and MCP-3 in Ulcerative Colitis Am. J. Pathol., August 1, 1999; 155(2): 331 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Wedemeyer, A Lorentz, M Goke, P N Meier, P Flemming, C A Dahinden, M P Manns, and S C Bischoff Enhanced production of monocyte chemotactic protein 3 in inflammatory bowel disease mucosa Gut, May 1, 1999; 44(5): 629 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Sansonetti, J. Arondel, M. Huerre, A. Harada, and K. Matsushima Interleukin-8 Controls Bacterial Transepithelial Translocation at the Cost of Epithelial Destruction in Experimental Shigellosis Infect. Immun., March 1, 1999; 67(3): 1471 - 1480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Song, K. Ito, T. L. Denning, D. Kuninger, J. Papaconstantinou, W. Gourley, G. Klimpel, E. Balish, J. Hokanson, and P. B. Ernst Expression of the Neutrophil Chemokine KC in the Colon of Mice with Enterocolitis and by Intestinal Epithelial Cell Lines: Effects of Flora and Proinflammatory Cytokines J. Immunol., February 15, 1999; 162(4): 2275 - 2280. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
