This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schrock, E. Articles by Janisch, W. Search for Related Content PubMed PubMed Citation Articles by Schrock, E. Articles by Janisch, W.

American Journal of Pathology, Vol 144, 1203-1218, Copyright © 1994 by American Society for Investigative Pathology

REGULAR ARTICLES

Comparative genomic hybridization of human malignant gliomas reveals multiple amplification sites and nonrandom chromosomal gains and losses

E Schrock, G Thiel, T Lozanova, S du Manoir, MC Meffert, A Jauch, MR Speicher, P Nurnberg, S Vogel and W Janisch
Institute of Human Genetics and Anthropology, University of Heidelberg, Germany.

Nine human malignant gliomas (2 astrocytomas grade III and 7 glioblastomas) were analyzed using comparative genomic hybridization (CGH). In addition to the amplification of the EGFR gene at 7p12 in 4 of 9 cases, six new amplification sites were mapped to 1q32, 4q12, 7q21.1, 7q21.2-3, 12p, and 22q12. Nonrandom chromosomal gains and losses were identified with overrepresentation of chromosome 7 and underrepresentation of chromosome 10 as the most frequent events (1 of 2 astrocytomas, 7 of 7 glioblastomas). Gain of a part or the whole chromosome 19 and losses of chromosome bands 9pter-23 and 22q13 were detected each in five cases. Loss of chromosome band 17p13 and gain of chromosome 20 were revealed each in three cases. The validity of the CGH data was confirmed using interphase cytogenetics with YAC clones, chromosome painting in tumor metaphase spreads, and DNA fingerprinting. A comparison of CGH data with the results of chromosome banding analyses indicates that metaphase spreads accessible in primary tumor cell cultures may not represent the clones predominant in the tumor tissue.

This article has been cited by other articles:

				U. Fischer, A. Keller, P. Leidinger, S. Deutscher, S. Heisel, S. Urbschat, H.-P. Lenhof, and E. Meese A Different View on DNA Amplifications Indicates Frequent, Highly Complex, and Stable Amplicons on 12q13-21 in Glioma Mol. Cancer Res., April 1, 2008; 6(4): 576 - 584. [Abstract] [Full Text] [PDF]

				G. Huang, S. Krig, D. Kowbel, H. Xu, B. Hyun, S. Volik, B. Feuerstein, G. B. Mills, D. Stokoe, P. Yaswen, et al. ZNF217 suppresses cell death associated with chemotherapy and telomere dysfunction Hum. Mol. Genet., November 1, 2005; 14(21): 3219 - 3225. [Abstract] [Full Text] [PDF]

				B. Suarez-Merino, M. Hubank, T. Revesz, W. Harkness, R. Hayward, D. Thompson, J. L. Darling, D. G.T. Thomas, and T. J. Warr Microarray analysis of pediatric ependymoma identifies a cluster of 112 candidate genes including four transcripts at 22q12.1-q13.3 Neuro-oncol, January 1, 2005; 7(1): 20 - 31. [Abstract] [PDF]

				R. N. Wiltshire, J. E. Herndon II, A. Lloyd, H. S. Friedman, D. D. Bigner, S. H. Bigner, and R. E. McLendon Comparative Genomic Hybridization Analysis of Astrocytomas: Prognostic and Diagnostic Implications J. Mol. Diagn., August 1, 2004; 6(3): 166 - 179. [Abstract] [Full Text]

				S. Raguz, M. Tamburo De Bella, G. Tripuraneni, M. J. Slade, C. F. Higgins, R. C. Coombes, and E. Yague Activation of the MDR1 Upstream Promoter in Breast Carcinoma as a Surrogate for Metastatic Invasion Clin. Cancer Res., April 15, 2004; 10(8): 2776 - 2783. [Abstract] [Full Text] [PDF]

				E. C. Burton, K. R. Lamborn, B. G. Feuerstein, M. Prados, J. Scott, P. Forsyth, S. Passe, R. B. Jenkins, and K. D. Aldape Genetic Aberrations Defined by Comparative Genomic Hybridization Distinguish Long-Term from Typical Survivors of Glioblastoma Cancer Res., November 1, 2002; 62(21): 6205 - 6210. [Abstract] [Full Text] [PDF]

				C. H. Rickert, R. Strater, P. Kaatsch, H. Wassmann, H. Jurgens, B. Dockhorn-Dworniczak, and W. Paulus Pediatric High-Grade Astrocytomas Show Chromosomal Imbalances Distinct from Adult Cases Am. J. Pathol., April 1, 2001; 158(4): 1525 - 1532. [Abstract] [Full Text] [PDF]

				A. E COCKWELL, B. GIBBONS, I. E MOORE, and J. A CROLLA An analphoid supernumerary marker chromosome derived from chromosome 3 ascertained in a fetus with multiple malformations J. Med. Genet., October 1, 2000; 37(10): 807 - 810. [Full Text]

				R. N. Wiltshire, B.K. A. Rasheed, H. S. Friedman, A. H. Friedman, and S. H. Bigner Comparative genetic patterns of glioblastoma multiforme: Potential diagnostic tool for tumor classification Neuro-oncol, July 1, 2000; 2(3): 164 - 173. [Abstract] [PDF]

				H. KNOBLAUCH, G. THIEL, S. TINSCHERT, H. KÖRNER, C. TENNSTEDT, R. CHAOUI, J. KOHLHASE, C. DIXKENS, and C. BLANCK Clinical and molecular cytogenetic studies of a large de novo interstitial deletion 16q11.2-16q21 including the putative transcription factor gene SALL1 J. Med. Genet., May 1, 2000; 37(5): 389 - 392. [Full Text]

				M. J. Riemenschneider, R. Buschges, M. Wolter, J. Reifenberger, J. Bostrom, J. A. Kraus, U. Schlegel, and G. Reifenberger Amplification and Overexpression of the MDM4 (MDMX) Gene from 1q32 in a Subset of Malignant Gliomas without TP53 Mutation or MDM2 Amplification Cancer Res., December 1, 1999; 59(24): 6091 - 6096. [Abstract] [Full Text] [PDF]

				S. L. Huhn, G. Mohapatra, A. Bollen, K. Lamborn, M. D. Prados, and B. G. Feuerstein Chromosomal Abnormalities in Glioblastoma Multiforme by Comparative Genomic Hybridization: Correlation with Radiation Treatment Outcome Clin. Cancer Res., June 1, 1999; 5(6): 1435 - 1443. [Abstract] [Full Text] [PDF]

				P. E. McKeever Insights About Brain Tumors Gained Through Immunohistochemistry and in Situ Hybridization of Nuclear and Phenotypic Markers J. Histochem. Cytochem., May 1, 1998; 46(5): 585 - 594. [Abstract] [Full Text]