| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 144, 1269-1280, Copyright © 1994 by American Society for Investigative Pathology
REGULAR ARTICLES |
BM Schafer, K Maier, U Eickhoff, RF Todd and MD Kramer
Institut fur Immunologie und Serologie, Universitat Heidelberg, Germany.
Tissue injury is followed by formation of a provisional, fibrin- containing matrix. It is later on replaced by granulation tissue. Replacement involves extracellular proteolysis by fibrinolytic enzymes. Plasmin is a fibrinolytic proteinase and is generated from ubiquitous plasminogen by cell-derived urokinase-type (uPA) or tissue-type (tPA) plasminogen activator. To explore the cells and components involved in plasminogen activation, we have performed a combined immunohistological and zymographic study on human skin wounds produced iatrogenically by debridement. The fibrin(ogen)-specific staining indicated the progressive removal of a fibrin-containing provisional matrix. Plasmin(ogen) was present over the entire observation period. It was diffusely distributed and also displayed a conspicuous association with cells of the granulation tissue, in particular with monocytes/macrophages and fibroblasts. Also, uPA was associated with monocytes/macrophages and fibroblasts, whereas the uPA-receptor (uPA-R) was stained in monocytes/macrophages only. The uPA was potentially active as indicated by zymography. No tPA-specific staining was found. The findings point at the importance of monocytes/macrophages and fibroblasts in uPA-mediated plasminogen activation in healing human skin wounds.
This article has been cited by other articles:
 |
|
 |
|
  G. Fitsialos, A.-A. Chassot, L. Turchi, M. A. Dayem, K. LeBrigand, C. Moreilhon, G. Meneguzzi, R. Busca, B. Mari, P. Barbry, et al. Transcriptional Signature of Epidermal Keratinocytes Subjected to in Vitro Scratch Wounding Reveals Selective Roles for ERK1/2, p38, and Phosphatidylinositol 3-Kinase Signaling Pathways J. Biol. Chem., May 18, 2007; 282(20): 15090 - 15102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.C. Smith and J. Martinez Differential uPA Expression by TGF-{beta}1 in Gingival Fibroblasts J. Dent. Res., February 1, 2006; 85(2): 150 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. F. Khan, L. R. Howe, and D. J. Falcone Extracellular Matrix-induced Cyclooxygenase-2 Regulates Macrophage Proteinase Expression J. Biol. Chem., May 21, 2004; 279(21): 22039 - 22046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Crowther, N. J. Brown, E. T. Bishop, and C. E. Lewis Microenvironmental influence on macrophage regulation of angiogenesis in wounds and malignant tumors J. Leukoc. Biol., October 1, 2001; 70(4): 478 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. B. Siconolfi and N. W. Seeds Induction of the Plasminogen Activator System Accompanies Peripheral Nerve Regeneration after Sciatic Nerve Crush J. Neurosci., June 15, 2001; 21(12): 4336 - 4347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Siconolfi and N. W. Seeds Mice Lacking tPA, uPA, or Plasminogen Genes Showed Delayed Functional Recovery after Sciatic Nerve Crush J. Neurosci., June 15, 2001; 21(12): 4348 - 4355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Falcone, W. Borth, K. M. F. Khan, and K. A. Hajjar Plasminogen-mediated matrix invasion and degradation by macrophages is dependent on surface expression of annexin II Blood, February 1, 2001; 97(3): 777 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-M. Deng, M. Ding, R. M. Lavker, and T.-T. Sun Urothelial function reconsidered: A role in urinary protein secretion PNAS, January 2, 2001; 98(1): 154 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Bickerstaff, D. Xia, R. P. Pelletier, and C. G. Orosz Mechanisms of Graft Acceptance: Evidence That Plasminogen Activator Controls Donor-Reactive Delayed-Type Hypersensitivity Responses in Cardiac Allograft Acceptor Mice J. Immunol., May 15, 2000; 164(10): 5132 - 5139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hattori, T. H. Sisson, Y. Xu, T. J. Desai, and R. H. Simon Participation of urokinase-type plasminogen activator receptor in the clearance of fibrin from the lung Am J Physiol Lung Cell Mol Physiol, September 1, 1999; 277(3): L573 - L579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Schott, C.-E. Dempfle, P. Beck, A. Liermann, A. Mohr-Pennert, M. Goldner, P. Mehlem, H. Azuma, V. Schuster, A.-M. Mingers, et al. Therapy with a Purified Plasminogen Concentrate in an Infant with Ligneous Conjunctivitis and Homozygous Plasminogen Deficiency N. Engl. J. Med., December 3, 1998; 339(23): 1679 - 1686. [Full Text] [PDF]
|
|
|
|
|
|
V. Schuster, A.-M. Mingers, S. Seidenspinner, Z. Nussgens, T. Pukrop, and H. W. Kreth Homozygous Mutations in the Plasminogen Gene of Two Unrelated Girls With Ligneous Conjunctivitis Blood, August 1, 1997; 90(3): 958 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R Garvin, M. Labinaz, K. Pels, V. M Walley, H. F Mizgala, and E. R O'Brien Arterial expression of the plasminogen activator system early after cardiac transplantation Cardiovasc Res, August 1, 1997; 35(2): 241 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M.F. Khan and D. J. Falcone Role of Laminin in Matrix Induction of Macrophage Urokinase-type Plasminogen Activator and 92-kDa Metalloproteinase Expression J. Biol. Chem., March 28, 1997; 272(13): 8270 - 8275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Mikhailenko, D. Krylov, K. M. Argraves, D. D. Roberts, G. Liau, and D. K. Strickland Cellular Internalization and Degradation of Thrombospondin-1 Is Mediated by the Amino-terminal Heparin Binding Domain (HBD). HIGH AFFINITY INTERACTION OF DIMERIC HBD WITH THE LOW DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN J. Biol. Chem., March 7, 1997; 272(10): 6784 - 6791. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
