This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cai, J. Articles by Radka, S. F. Search for Related Content PubMed PubMed Citation Articles by Cai, J. Articles by Radka, S. F.

American Journal of Pathology, Vol 145, 74-79, Copyright © 1994 by American Society for Investigative Pathology

REGULAR ARTICLES

Oncostatin-M is an autocrine growth factor in Kaposi's sarcoma

J Cai, PS Gill, R Masood, P Chandrasoma, B Jung, RE Law and SF Radka
Department of Medicine and Pathology, University of Southern California School of Medicine, Los Angeles.

Oncostatin-M is a cytokine produced by macrophages and activated T lymphocytes that has recently been shown to be a mitogen for AIDS- related Kaposi's sarcoma (KS)-derived spindle cells. The significance of oncostatin-M production in AIDS-related KS in vivo, however, remains unknown. In this study we wanted to determine whether oncostatin-M is expressed in vivo in patients with HIV-I-related KS, define the cell types that express this cytokine, and compared with the control tissues from HIV-I-negative individuals. A second objective of our study was to define the expression of oncostatin-M in AIDS-KS-derived spindle cell isolates cultured in vitro and to determine whether oncostatin-M is an autocrine growth factor for these KS cells. We have determined that oncostatin-M is not expressed in any of the several organs examined in control cases, whereas the tumor tissue obtained from the skin biopsies of HIV-I-infected cases with KS displayed oncostatin-M expression in the spindle cell components of the tumor, as well as the cells lining the vascular structures, smooth muscle cells lining the eccrine sweat glands, and the epidermal layers of the skin. Furthermore, uninvolved skin of patients with HIV-related KS express oncostatin-M in the cells lining normal vessels. The mRNA polymerase chain reaction analysis confirmed findings in the primary tissues and showed expression in all of the AIDS-KS-derived spindle cell isolates examined. We have also shown with the use of oncostatin-M-specific antisense oligodeoxynucleotides that KS cell proliferation is inhibited, which correlated with a more precipitous decline in the production of interleukin-6 by these cells. We conclude that oncostatin-M is only expressed in the skin and KS tumor of HIV-I-infected individuals. Furthermore, we provide evidence that oncostatin-M is an autocrine growth factor for KS.

This article has been cited by other articles:

				A. S. Punjabi, P. A. Carroll, L. Chen, and M. Lagunoff Persistent Activation of STAT3 by Latent Kaposi's Sarcoma-Associated Herpesvirus Infection of Endothelial Cells J. Virol., March 1, 2007; 81(5): 2449 - 2458. [Abstract] [Full Text] [PDF]

				E. E. Brown, D. Fallin, I. Ruczinski, A. Hutchinson, B. Staats, F. Vitale, C. Lauria, D. Serraino, G. Rezza, G. Mbisa, et al. Associations of Classic Kaposi Sarcoma with Common Variants in Genes that Modulate Host Immunity. Cancer Epidemiol. Biomarkers Prev., May 1, 2006; 15(5): 926 - 934. [Abstract] [Full Text] [PDF]

				L. A. Dourmishev, A. L. Dourmishev, D. Palmeri, R. A. Schwartz, and D. M. Lukac Molecular Genetics of Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Epidemiology and Pathogenesis Microbiol. Mol. Biol. Rev., June 1, 2003; 67(2): 175 - 212. [Abstract] [Full Text] [PDF]

				K. Mahboubi and J. S. Pober Activation of Signal Transducer and Activator of Transcription 1 (STAT1) Is Not Sufficient for the Induction of STAT1-dependent Genes in Endothelial Cells. COMPARISON OF INTERFERON-gamma AND ONCOSTATIN M J. Biol. Chem., March 1, 2002; 277(10): 8012 - 8021. [Abstract] [Full Text] [PDF]

				R. Somasundaram, M. Ruehl, B. Schaefer, M. Schmid, R. Ackermann, E. O. Riecken, M. Zeitz, and D. Schuppan Interstitial Collagens I, III, and VI Sequester and Modulate the Multifunctional Cytokine Oncostatin M J. Biol. Chem., January 25, 2002; 277(5): 3242 - 3246. [Abstract] [Full Text] [PDF]

				R. Masood, J. Cai, A. Tulpule, T. Zheng, A. Hamilton, S. Sharma, B. M. Espina, D. L. Smith, and P. S. Gill Interleukin 8 Is an Autocrine Growth Factor and a Surrogate Marker for Kaposi's Sarcoma Clin. Cancer Res., September 1, 2001; 7(9): 2693 - 2702. [Abstract] [Full Text] [PDF]

				I. Ogata, K. Shimoya, A. Moriyama, Y. Shiki, Y. Matsumura, K. Yamanaka, T. Nobunaga, Y. Tokugawa, T. Kimura, M. Koyama, et al. Oncostatin M is produced during pregnancy by decidual cells and stimulates the release of HCG Mol. Hum. Reprod., August 1, 2000; 6(8): 750 - 757. [Abstract] [Full Text] [PDF]

				M. Mercader, B. Taddeo, J. R. Panella, B. Chandran, B. J. Nickoloff, and K. E. Foreman Induction of HHV-8 Lytic Cycle Replication by Inflammatory Cytokines Produced by HIV-1-Infected T Cells Am. J. Pathol., June 1, 2000; 156(6): 1961 - 1971. [Abstract] [Full Text] [PDF]

				N. Munshi, J. E. Groopman, P. S. Gill, and R. K. Ganju c-Src Mediates Mitogenic Signals and Associates with Cytoskeletal Proteins upon Vascular Endothelial Growth Factor Stimulation in Kaposi's Sarcoma Cells J. Immunol., February 1, 2000; 164(3): 1169 - 1174. [Abstract] [Full Text] [PDF]

				C. Bernard, R. Merval, M. Lebret, P. Delerive, I. Dusanter-Fourt, S. Lehoux, C. Creminon, B. Staels, J. Maclouf, and A. Tedgui Oncostatin M Induces Interleukin-6 and Cyclooxygenase-2 Expression in Human Vascular Smooth Muscle Cells : Synergy With Interleukin-1{beta} Circ. Res., December 3, 1999; 85(12): 1124 - 1131. [Abstract] [Full Text] [PDF]

				R. Sun, S.-F. Lin, K. Staskus, L. Gradoville, E. Grogan, A. Haase, and G. Miller Kinetics of Kaposi's Sarcoma-Associated Herpesvirus Gene Expression J. Virol., March 1, 1999; 73(3): 2232 - 2242. [Abstract] [Full Text]

				V. Fiorelli, G. Barillari, E. Toschi, C. Sgadari, P. Monini, M. Sturzl, and B. Ensoli IFN-{gamma} Induces Endothelial Cells to Proliferate and to Invade the Extracellular Matrix in Response to the HIV-1 Tat Protein: Implications for AIDS-Kaposi's Sarcoma Pathogenesis J. Immunol., January 15, 1999; 162(2): 1165 - 1170. [Abstract] [Full Text] [PDF]

				R. K. Ganju, N. Munshi, B. C. Nair, Z.-Y. Liu, P. Gill, and J. E. Groopman Human Immunodeficiency Virus Tat Modulates the Flk-1/KDR Receptor, Mitogen-Activated Protein Kinases, and Components of Focal Adhesion in Kaposi's Sarcoma Cells J. Virol., July 1, 1998; 72(7): 6131 - 6137. [Abstract] [Full Text] [PDF]

				J. Nicholas, J.-C. Zong, D. J. Alcendor, D. M. Ciufo, L. J. Poole, R. T. Sarisky, C.-J. Chiou, X. Zhang, X. Wan, H.-G. Guo, et al. Novel Organizational Features, Captured Cellular Genes, and Strain Variability Within the Genome of KSHV/HHV8 J Natl Cancer Inst Monographs, April 1, 1998; 1998(23): 79 - 88. [Abstract] [Full Text]

				M. C. Sirianni, L. Vincenzi, V. Fiorelli, S. Topino, E. Scala, S. Uccini, A. Angeloni, A. Faggioni, D. Cerimele, F. Cottoni, et al. gamma -Interferon Production in Peripheral Blood Mononuclear Cells and Tumor Infiltrating Lymphocytes From Kaposi's Sarcoma Patients: Correlation With the Presence of Human Herpesvirus-8 in Peripheral Blood Mononuclear Cells and Lesional Macrophages Blood, February 1, 1998; 91(3): 968 - 976. [Abstract] [Full Text] [PDF]

				R. Masood, J. Cai, T. Zheng, D. L. Smith, Y. Naidu, and P. S. Gill Vascular endothelial growth factor/vascular permeability factor is an autocrine growth factor for AIDS-Kaposi sarcoma PNAS, February 4, 1997; 94(3): 979 - 984. [Abstract] [Full Text] [PDF]

				B. Mosley, C. De Imus, D. Friend, N. Boiani, B. Thoma, L. S. Park, and D. Cosman Dual Oncostatin M (OSM) Receptors. CLONING AND CHARACTERIZATION OF AN ALTERNATIVE SIGNALING SUBUNIT CONFERRING OSM-SPECIFIC RECEPTOR ACTIVATION J. Biol. Chem., December 20, 1996; 271(51): 32635 - 32643. [Abstract] [Full Text] [PDF]