| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 145, 913-921, Copyright © 1994 by American Society for Investigative Pathology
REGULAR ARTICLES |
Z Brown, ME Gerritsen, WW Carley, RM Strieter, SL Kunkel and J Westwick
Department of Pharmacology, University of Bath, Avon, United Kingdom.
The elicitation of leukocytes from the circulation to inflamed tissue depends on the activation of both the leukocyte and endothelial cell. In this study we determined the gene expression and secretion patterns for the chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in cytokine- and lipopolysaccharide (LPS)-treated cultured human lung microvascular endothelial cells (HLE). HLE constitutively expressed low levels of MCP-1 and IL-8. Treatment of HLE with a variety of cytokines and LPS up-regulated both IL-8 mRNA expression and release of immunoreactive IL-8 with an order of potency tumor necrosis factor-alpha (TNF-alpha) >> IL-1 alpha > LPS, whereas interferon-gamma (IFN-gamma) had no effect on IL-8 mRNA or antigenic levels. However, IFN-gamma, in combination with high doses of IL-1 alpha, resulted in a synergistic increase in IL-8 generation. MCP-1 gene expression and secretion was induced in a dose-dependent manner after IL-1 alpha, TNF-alpha, IFN-gamma, and LPS activation of HLE. IL-1 alpha was the most potent inducer of MCP-1 generation and LPS was relatively ineffective. IFN-gamma, in combination with low doses of IL- 1 alpha, resulted in a synergistic increase in MCP-1 generation by HLE. These results demonstrate that although IL-8 and MCP-1 generation by HLE occurs on cytokine treatment, the relative ability of a given cytokine to elicit IL-8 generation is not directly parallel to effects on MCP-1 generation. These data suggest that the regulation of IL-8 and MCP-1 expression exhibit significant differences in their mechanisms. Such differences in the expression of specific chemokines may explain the specific appearance of various leukocytes at sites of inflammation and injury. These data also directly demonstrate that the lung microvascular endothelium contribute to the cytokine network of the lung, with the ability to respond to locally generated cytokines and to produce potent mediators of the local inflammatory response.
This article has been cited by other articles:
 |
|
 |
|
  R.-N. E. Dogan, A. Elhofy, and W. J. Karpus Production of CCL2 by Central Nervous System Cells Regulates Development of Murine Experimental Autoimmune Encephalomyelitis through the Recruitment of TNF- and iNOS-Expressing Macrophages and Myeloid Dendritic Cells J. Immunol., June 1, 2008; 180(11): 7376 - 7384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nagao, M. Matsumura, A. Mabuchi, A. Ishida-Okawara, O. Koshio, T. Nakayama, H. Minamitani, and K. Suzuki Up-regulation of adhesion molecule expression in glomerular endothelial cells by anti-myeloperoxidase antibody Nephrol. Dial. Transplant., January 1, 2007; 22(1): 77 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Quehenberger Thematic Review Series: The Immune System and Atherogenesis. Molecular mechanisms regulating monocyte recruitment in atherosclerosis J. Lipid Res., August 1, 2005; 46(8): 1582 - 1590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pertosa, S. Simone, M. Soccio, D. Marrone, L. Gesualdo, F. P. Schena, and G. Grandaliano Coagulation Cascade Activation Causes CC Chemokine Receptor-2 Gene Expression and Mononuclear Cell Activation in Hemodialysis Patients J. Am. Soc. Nephrol., August 1, 2005; 16(8): 2477 - 2486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Kronke, V. N. Bochkov, J. Huber, F. Gruber, S. Bluml, A. Furnkranz, A. Kadl, B. R. Binder, and N. Leitinger Oxidized Phospholipids Induce Expression of Human Heme Oxygenase-1 Involving Activation of cAMP-responsive Element-binding Protein J. Biol. Chem., December 19, 2003; 278(51): 51006 - 51014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. I. Majeski, M. K. Paintlia, A. D. Lopez, R. A. Harley, S. D. London, and L. London Respiratory Reovirus 1/L Induction of Intraluminal Fibrosis, a Model of Bronchiolitis Obliterans Organizing Pneumonia, Is Dependent on T Lymphocytes Am. J. Pathol., October 1, 2003; 163(4): 1467 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Tay, A. McCormack, C. Lawson, and M. L. Rose IFN-{gamma} Reverses the Stop Signal Allowing Migration of Antigen-Specific T Cells into Inflammatory Sites J. Immunol., March 15, 2003; 170(6): 3315 - 3322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Watari, H. Watari, T. Fujimoto, H. Yamada, J. Nishihira, J. f. Strauss III, and S. Fujimoto Lipopolysaccharide Induces Interleukin-8 Production By Human Cervical Smooth Muscle Cells Reproductive Sciences, February 1, 2003; 10(2): 110 - 117. [Abstract] [PDF]
|
|
|
|
 |
|
 E. I. Majeski, R. A. Harley, S. C. Bellum, S. D. London, and L. London Differential Role for T Cells in the Development of Fibrotic Lesions Associated with Reovirus 1/L-Induced Bronchiolitis Obliterans Organizing Pneumonia versus Acute Respiratory Distress Syndrome Am. J. Respir. Cell Mol. Biol., February 1, 2003; 28(2): 208 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Saukkonen, B. Bazydlo, M. Thomas, R. M. Strieter, J. Keane, and H. Kornfeld {beta}-Chemokines Are Induced by Mycobacterium tuberculosis and Inhibit Its Growth Infect. Immun., April 1, 2002; 70(4): 1684 - 1693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Diks, S. J.H. van Deventer, and M. P. Peppelenbosch Invited review: Lipopolysaccharide recognition, internalisation, signalling and other cellular effects Innate Immunity, October 1, 2001; 7(5): 335 - 348. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Masood, J. Cai, A. Tulpule, T. Zheng, A. Hamilton, S. Sharma, B. M. Espina, D. L. Smith, and P. S. Gill Interleukin 8 Is an Autocrine Growth Factor and a Surrogate Marker for Kaposi's Sarcoma Clin. Cancer Res., September 1, 2001; 7(9): 2693 - 2702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Blease, Y. Chen, P. G. Hellewell, and A. Burke-Gaffney Lipoteichoic Acid inhibits Lipopolysaccharide-Induced Adhesion Molecule Expression and IL-8 Release in Human Lung Microvascular Endothelial Cells J. Immunol., December 1, 1999; 163(11): 6139 - 6147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. O. D. Critchley, R. L. Jones, R. G. Lea, T. A. Drudy, R. W. Kelly, A. R. W. Williams, and D. T. Baird Role of Inflammatory Mediators in Human Endometrium during Progesterone Withdrawal and Early Pregnancy J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 240 - 248. [Abstract] [Full Text]
|
|
|
|
|
|
C. A. Cuff, J. Schwartz, C. M. Bergman, K. S. Russell, J. R. Bender, and N. H. Ruddle Lymphotoxin {alpha}3 Induces Chemokines and Adhesion Molecules: Insight into the Role of LT{alpha} in Inflammation and Lymphoid Organ Development J. Immunol., December 15, 1998; 161(12): 6853 - 6860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Han, R. K. Tangirala, S. R. Green, and O. Quehenberger Chemokine Receptor CCR2 Expression and Monocyte Chemoattractant Protein-1–Mediated Chemotaxis in Human Monocytes : A Regulatory Role for Plasma LDL Arterioscler. Thromb. Vasc. Biol., December 1, 1998; 18(12): 1983 - 1991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fujisawa, S. Hayashi, A. Kurdowska, J. M. Noble, K. Naitoh, and E. J. Miller Staphylococcal Enterotoxin A-Induced Injury of Human Lung Endothelial Cells and IL-8 Accumulation Are Mediated by TNF-{alpha} J. Immunol., November 15, 1998; 161(10): 5627 - 5632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Youssef, G. Wildbaum, G. Maor, N. Lanir, A. Gour-Lavie, N. Grabie, and N. Karin Long-Lasting Protective Immunity to Experimental Autoimmune Encephalomyelitis Following Vaccination with Naked DNA Encoding C-C Chemokines J. Immunol., October 15, 1998; 161(8): 3870 - 3879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Melrose, N. Tsurushita, G. Liu, and E. L. Berg IFN-{gamma} Inhibits Activation-Induced Expression of E- and P-Selectin on Endothelial Cells J. Immunol., September 1, 1998; 161(5): 2457 - 2464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E M Nilsen, F-E Johansen, F L Jahnsen, K E A Lundin, T Scholz, P Brandtzaeg, and G Haraldsen Cytokine profiles of cultured microvascular endothelial cells from the human intestine Gut, May 1, 1998; 42(5): 635 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Hayes, N. J. Jordan, S. Towers, G. Smith, J. R. Paterson, J. J. Earnshaw, A. G. Roach, J. Westwick, and R. J. Williams Human Vascular Smooth Muscle Cells Express Receptors for CC Chemokines Arterioscler. Thromb. Vasc. Biol., March 1, 1998; 18(3): 397 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Biswas, F. Delfanti, S. Bernasconi, M. Mengozzi, M. Cota, N. Polentarutti, A. Mantovani, A. Lazzarin, S. Sozzani, and G. Poli Interleukin-6 Induces Monocyte Chemotactic Protein-1 in Peripheral Blood Mononuclear Cells and in the U937 Cell Line Blood, January 1, 1998; 91(1): 258 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Wempe, V. Lindner, and H. G. Augustin Basic Fibroblast Growth Factor (bFGF) Regulates the Expression of the CC Chemokine Monocyte Chemoattractant Protein-1 (MCP-1) in Autocrine-Activated Endothelial Cells Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 2471 - 2478. [Abstract] [Full Text]
|
|
|
|
|
|
T.-a. Imaizumi, K. H. Albertine, D. L. Jicha, T. M. McIntyre, S. M. Prescott, and G. A. Zimmerman Human Endothelial Cells Synthesize ENA-78: Relationship to IL-8 and to Signaling of PMN Adhesion Am. J. Respir. Cell Mol. Biol., August 1, 1997; 17(2): 181 - 192. [Abstract] [Full Text]
|
|
|
|
|
|
C. Garlanda and E. Dejana Heterogeneity of Endothelial Cells : Specific Markers Arterioscler. Thromb. Vasc. Biol., July 1, 1997; 17(7): 1193 - 1202. [Abstract] [Full Text]
|
|
|
|
 |
|
 F.-J. Neumann, N. Marx, M. Gawaz, K. Brand, I. Ott, C. Rokitta, C. Sticherling, C. Meinl, A. May, and A. Schomig Induction of Cytokine Expression in Leukocytes by Binding of Thrombin-Stimulated Platelets Circulation, May 20, 1997; 95(10): 2387 - 2394. [Abstract] [Full Text]
|
|
|
|
|
|
R. K. Tangirala, K. Murao, and O. Quehenberger Regulation of Expression of the Human Monocyte Chemotactic Protein-1 Receptor (hCCR2) by Cytokines J. Biol. Chem., March 21, 1997; 272(12): 8050 - 8056. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
