help button home button Am J Pathol International Conference on Pathology of Chest Diseases
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Su, L.
Right arrow Articles by Lane, E. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Su, L.
Right arrow Articles by Lane, E. B.

American Journal of Pathology, Vol 145, 1349-1357, Copyright © 1994 by American Society for Investigative Pathology

REGULAR ARTICLES

Protein and mRNA expression of simple epithelial keratins in normal, dysplastic, and malignant oral epithelia

L Su, PR Morgan and EB Lane
Department of Oral Medicine and Pathology, United Medical School, Guy's Hospital, London, United Kingdom.

Simple epithelial keratins K7, K8, and K18 are present in no more than trace amounts in normal stratified squamous epithelial but have been reported in squamous cell carcinomas. With the aim of determining the level at which keratin synthesis is regulated in vivo, we have compared the expression of mRNA by in situ hybridization and protein by immunohistochemistry for K7, K8, and K18 in a series of normal, dysplastic, and malignant oral epithelia. In normal epithelia mRNAs for K7, K8, and K18 were present in basal and lower spinous cells but adjacent sections were generally negative for the respective proteins. In severe dysplasia there was irregular suprabasal extension of K8 and K18 mRNAs in all cases and their proteins were expressed in more than half of the cases. The carcinomas expressed K8 and K18 mRNAs homogeneously and were strongly reactive for these keratin proteins but K7 expression appeared reduced in malignancy. These results are consistent with the post-transcriptional regulation of K7, K8, and K18 expression in normal epithelia and the presence of their proteins in dysplastic and malignant epithelia suggests the release of these epithelial cells from a post-transcriptional block on K8 and K18 translation. Alternatively, rapid degradation of K8 and K18 protein might be occurring in normal epithelia but be suppressed in dysplasia and malignancy.


This article has been cited by other articles:


Home page
 Crit. Rev. Oral Biol. Med.Home page
J. Reibel
PROGNOSISOF ORAL PRE-MALIGNANT LESIONS: SIGNIFICANCEOF CLINICAL, HISTOPATHOLOGICAL, AND MOLECULAR BIOLOGICAL CHARACTERISTICS
Crit. Rev. Oral. Biol. Med., January 1, 2003; 14(1): 47 - 62.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Crit. Care Med.Home page
F. P. Paulsen, P. Steven, M. Tsokos, K. Jungmann, A. Muller, T. Verse, and W. Pirsig
Upper Airway Epithelial Structural Changes in Obstructive Sleep-disordered Breathing
Am. J. Respir. Crit. Care Med., August 15, 2002; 166(4): 501 - 509.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Society for Investigative Pathology.