| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 145, 1485-1495, Copyright © 1994 by American Society for Investigative Pathology
REGULAR ARTICLES |
DM Morrow, N Xiong, RR Getty, MZ Ratajczak, D Morgan, M Seppala, L Riittinen, AM Gewirtz and ML Tykocinski
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106-4943.
Placental protein 14 (PP14), an immunosuppressive molecule previously known to be expressed in the female and male reproductive tracts only, was shown to be expressed by hematopoietic cells of the megakaryocytic lineage. Northern blot analysis confirmed the induction specificity of PP14 mRNA in phorbol ester-treated K562 cells. Potent immunosuppressive activity in conditioned medium from phorbol ester-treated K562 cells was attributed to hematopoietic PP14 by anti-PP14 antibody blocking. Immunoprecipitation with anti-PP14 antibodies from conditioned medium revealed two distinct PP14 protein isoforms, designated PP14.1 and PP14.2. Polymerase chain reaction cloning and analysis demonstrated the presence of distinct mRNA counterparts to PP14.1 and PP14.2 that had not been resolved by Northern blot analyses. Hematopoietic PP14.1 mRNA corresponds in size to endometrial PP14 mRNA, whereas the smaller hematopoietic PP14.2 mRNA displays an internal in-frame 66-nucleotide deletion that can be explained by alternative splicing and predicts a 22-amino-acid deletion in the encoded gene product. Both PP14 mRNA isoforms were additionally detected by reverse transcriptase polymerase chain reaction analyses in two human megakaryocytic cell lines and in normal human megakaryocytes and platelets. PP14 mRNA was not detected by reverse transcriptase polymerase chain reaction in a panel of nonhematopoietic, nonendometrial tissues examined. The finding of hematopoietic PP14 within the megakaryocytic lineage provides an additional regulatory link between the coagulation and immune systems in normal and pathological settings.
This article has been cited by other articles:
 |
|
 |
|
  M. Seppala, H. Koistinen, R. Koistinen, P.C.N. Chiu, and W.S.B. Yeung Glycosylation related actions of glycodelin: gamete, cumulus cell, immune cell and clinical associations Hum. Reprod. Update, May 1, 2007; 13(3): 275 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lapid and N. Sharon Meet the multifunctional and sexy glycoforms of glycodelin Glycobiology, March 1, 2006; 16(3): 39R - 45R. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Mishan-Eisenberg, Z. Borovsky, M. C. Weber, R. Gazit, M. L. Tykocinski, and J. Rachmilewitz Differential Regulation of Th1/Th2 Cytokine Responses by Placental Protein 14 J. Immunol., November 1, 2004; 173(9): 5524 - 5530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rachmilewitz, Z. Borovsky, G. J. Riely, R. Miller, and M. L. Tykocinski Negative Regulation of T Cell Activation by Placental Protein 14 Is Mediated by the Tyrosine Phosphatase Receptor CD45 J. Biol. Chem., April 11, 2003; 278(16): 14059 - 14065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Seppala, R. N. Taylor, H. Koistinen, R. Koistinen, and E. Milgrom Glycodelin: A Major Lipocalin Protein of the Reproductive Axis with Diverse Actions in Cell Recognition and Differentiation Endocr. Rev., August 1, 2002; 23(4): 401 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rachmilewitz, Z. Borovsky, G. Mishan-Eisenberg, E. Yaniv, G. J. Riely, and M. L. Tykocinski Focal Localization of Placental Protein 14 Toward Sites of TCR Engagement J. Immunol., March 15, 2002; 168(6): 2745 - 2750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rachmilewitz, G. J. Riely, J.-H. Huang, A. Chen, and M. L. Tykocinski A rheostatic mechanism for T-cell inhibition based on elevation of activation thresholds Blood, December 15, 2001; 98(13): 3727 - 3732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Song, S. Ramaswamy, S. Ramachandran, L. C. Flowers, I. R. Horowitz, J. A. Rock, and S. Parthasarathy Angiogenic role for glycodelin in tumorigenesis PNAS, July 13, 2001; (2001) 151151198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Goldfarb, D. Wong, and F. K. Racke Induction of Megakaryocytic Differentiation in Primary Human Erythroblasts : A Physiological Basis for Leukemic Lineage Plasticity Am. J. Pathol., April 1, 2001; 158(4): 1191 - 1198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kalidas, L. Joshi, and C. Batt Characterization of glycosylated variants of {beta}-lactoglobulin expressed in Pichia pastoris Protein Eng. Des. Sel., March 1, 2001; 14(3): 201 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lacazette, A.-M. Gachon, and G. Pitiot A novel human odorant-binding protein gene family resulting from genomic duplicons at 9q34: differential expression in the oral and genital spheres Hum. Mol. Genet., January 22, 2000; 9(2): 289 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Magert, A. Cieslak, O. Alkan, B. Luscher, W. Kauffels, and W.-G. Forssmann The Golden Hamster Aphrodisin Gene. STRUCTURE, EXPRESSION IN PAROTID GLANDS OF FEMALE ANIMALS, AND COMPARISON WITH A SIMILAR MURINE GENE J. Biol. Chem., January 1, 1999; 274(1): 444 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. N. Taylor, J.-F. Savouret, C. Vaisse, J.-L. Vigne, I. Ryan, D. Hornung, M. Seppälä, and E. Milgrom Promegestone (R5020) and Mifepristone (RU486) Both Function as Progestational Agonists of Human Glycodelin Gene Expression in Isolated Human Epithelial Cells J. Clin. Endocrinol. Metab., November 1, 1998; 83(11): 4006 - 4012. [Abstract] [Full Text]
|
|
|
|
|
|
J. Rachmilewitz and M. L. Tykocinski Differential Effects of Chondroitin Sulfates A and B on Monocyte and B-Cell Activation: Evidence for B-Cell Activation Via a CD44-Dependent Pathway Blood, July 1, 1998; 92(1): 223 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ratajczak, J Ratajczak, J Ford, R Kregenow, W Marlicz, and A. Gewirtz FLT3/FLK-2 (STK-1) Ligand does not stimulate human megakaryopoiesis in vitro Stem Cells, January 1, 1996; 14(1): 146 - 150. [Abstract]
|
|
|
|
|
|
A. Dell, H. R. Morris, R. L. Easton, M. Panico, M. Patankar, S. Oehninger, R. Koistinen, H. Koistinen, M. Seppala, and G. F. Clark Structural Analysis of the Oligosaccharides Derived from Glycodelin, a Human Glycoprotein with Potent Immunosuppressive and Contraceptive Activities J. Biol. Chem., October 13, 1995; 270(41): 24116 - 24126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Vigne, D. Hornung, M. D. Mueller, and R. N. Taylor Purification and Characterization of an Immunomodulatory Endometrial Protein, Glycodelin J. Biol. Chem., May 11, 2001; 276(20): 17101 - 17105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Song, S. Ramaswamy, S. Ramachandran, L. C. Flowers, I. R. Horowitz, J. A. Rock, and S. Parthasarathy Angiogenic role for glycodelin in tumorigenesis PNAS, July 31, 2001; 98(16): 9265 - 9270. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
