help button home button Am J Pathol ASIP 2008 Summer Academy, Molecular Methcanisms of Human Disease: Injury, Inflammation, and Tissue Repair
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brieland, J.
Right arrow Articles by Engleberg, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brieland, J.
Right arrow Articles by Engleberg, C.

American Journal of Pathology, Vol 145, 1537-1546, Copyright © 1994 by American Society for Investigative Pathology

REGULAR ARTICLES

Replicative Legionella pneumophila lung infection in intratracheally inoculated A/J mice. A murine model of human Legionnaires' disease

J Brieland, P Freeman, R Kunkel, C Chrisp, M Hurley, J Fantone and C Engleberg
Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor.

The role of host immune responses in the pathogenesis of Legionnaires' disease is incompletely understood, due in part to the current lack of an animal model that is both susceptible to replicative Legionella pneumophila-induced lung infection and for which species-specific immunological reagents are available. We have developed a model of replicative L. pneumophila lung infection in intratracheally inoculated A/J mice. L. pneumophila was obtained in the exponential growth phase and inoculated into the trachea of 6- to 8-week-old female A/J mice. Microbiological and histopathological evidence of infection was demonstrated in mice inoculated with 10(6) colony-forming units. Development of an acute pneumonia that resembled human Legionnaires' disease coincided with exponential growth of the bacteria in the lung 24 to 48 hours after intratracheal inoculation of L. pneumophila. This was associated with increased plasma levels of interferon-gamma at 24 hours after inoculation. After 48 hours, the bacteria were gradually eliminated from the lung over the next 5 days, corresponding with resolution of the inflammatory response in the lung, thereby mimicking the outcome frequently seen in the immunocompetent human host. Treatment of animals with anti-interferon-gamma antibody enhanced bacterial replication and disease progression, indicating an important role of host immune response in resolution of the infection. Because of the availability of murine-specific reagents, this model of replicative L. pneumophila lung infection in A/J mice after intrapulmonary inoculation of L. pneumophila potentially provides an important tool for future studies investigating the role of host immune responses in the pathogenesis of Legionnaires' disease in the immunocompetent host.


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
U. Bhan, G. Trujillo, K. Lyn-Kew, M. W. Newstead, X. Zeng, C. M. Hogaboam, A. M. Krieg, and T. J. Standiford
Toll-Like Receptor 9 Regulates the Lung Macrophage Phenotype and Host Immunity in Murine Pneumonia Caused by Legionella pneumophila
Infect. Immun., July 1, 2008; 76(7): 2895 - 2904.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. M. Sansom, P. Riedmaier, H. J. Newton, M. A. Dunstone, C. E. Muller, H. Stephan, E. Byres, T. Beddoe, J. Rossjohn, P. J. Cowan, et al.
Enzymatic Properties of an Ecto-nucleoside Triphosphate Diphosphohydrolase from Legionella pneumophila: SUBSTRATE SPECIFICITY AND REQUIREMENT FOR VIRULENCE
J. Biol. Chem., May 9, 2008; 283(19): 12909 - 12918.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Kunz, K. Oberle, A. Sander, C. Bogdan, and U. Schleicher
Lymphadenopathy in a Novel Mouse Model of Bartonella-Induced Cat Scratch Disease Results from Lymphocyte Immigration and Proliferation and Is Regulated by Interferon-{alpha}/{beta}
Am. J. Pathol., April 1, 2008; 172(4): 1005 - 1018.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. P. Mizgerd and S. J. Skerrett
Animal models of human pneumonia
Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L387 - L398.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. Asare, M. Santic, I. Gobin, M. Doric, J. Suttles, J. E. Graham, C. D. Price, and Y. Abu Kwaik
Genetic Susceptibility and Caspase Activation in Mouse and Human Macrophages Are Distinct for Legionella longbeachae and L. pneumophila
Infect. Immun., April 1, 2007; 75(4): 1933 - 1945.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
B. Schmeck, P. D. N'Guessan, M. Ollomang, J. Lorenz, J. Zahlten, B. Opitz, A. Flieger, N. Suttorp, and S. Hippenstiel
Legionella pneumophila-induced NF-{kappa}B- and MAPK-dependent cytokine release by lung epithelial cells
Eur. Respir. J., January 1, 2007; 29(1): 25 - 33.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Amer, L. Franchi, T.-D. Kanneganti, M. Body-Malapel, N. Ozoren, G. Brady, S. Meshinchi, R. Jagirdar, A. Gewirtz, S. Akira, et al.
Regulation of Legionella Phagosome Maturation and Infection through Flagellin and Host Ipaf
J. Biol. Chem., November 17, 2006; 281(46): 35217 - 35223.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. A. Archer and C. R. Roy
MyD88-Dependent Responses Involving Toll-Like Receptor 2 Are Important for Protection and Clearance of Legionella pneumophila in a Mouse Model of Legionnaires' Disease.
Infect. Immun., June 1, 2006; 74(6): 3325 - 3333.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Sporri, N. Joller, U. Albers, H. Hilbi, and A. Oxenius
MyD88-Dependent IFN-{gamma} Production by NK Cells Is Key for Control of Legionella pneumophila Infection
J. Immunol., May 15, 2006; 176(10): 6162 - 6171.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
A. B. Molofsky, B. G. Byrne, N. N. Whitfield, C. A. Madigan, E. T. Fuse, K. Tateda, and M. S. Swanson
Cytosolic recognition of flagellin by mouse macrophages restricts Legionella pneumophila infection
J. Exp. Med., April 17, 2006; 203(4): 1093 - 1104.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. A. Allard, V. K. Viswanathan, and N. P. Cianciotto
lbtA and lbtB Are Required for Production of the Legionella pneumophila Siderophore Legiobactin
J. Bacteriol., February 15, 2006; 188(4): 1351 - 1363.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. L. Neild, S. Shin, and C. R. Roy
Activated Macrophages Infected with Legionella Inhibit T Cells by Means of MyD88-Dependent Production of Prostaglandins
J. Immunol., December 15, 2005; 175(12): 8181 - 8190.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. B. Molofsky, L. M. Shetron-Rama, and M. S. Swanson
Components of the Legionella pneumophila Flagellar Regulon Contribute to Multiple Virulence Traits, Including Lysosome Avoidance and Macrophage Death
Infect. Immun., September 1, 2005; 73(9): 5720 - 5734.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Yoshizawa, K. Tateda, T. Matsumoto, F. Gondaira, S. Miyazaki, T. J. Standiford, and K. Yamaguchi
Legionella pneumophila Evades Gamma Interferon-Mediated Growth Suppression through Interleukin-10 Induction in Bone Marrow-Derived Macrophages
Infect. Immun., May 1, 2005; 73(5): 2709 - 2717.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Schiavoni, C. Mauri, D. Carlei, F. Belardelli, M. Castellani Pastoris, and E. Proietti
Type I IFN Protects Permissive Macrophages from Legionella pneumophila Infection through an IFN-{gamma}-Independent Pathway
J. Immunol., July 15, 2004; 173(2): 1266 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Kikuchi, T. Kobayashi, K. Gomi, T. Suzuki, Y. Tokue, A. Watanabe, and T. Nukiwa
Dendritic Cells Pulsed with Live and Dead Legionella pneumophila Elicit Distinct Immune Responses
J. Immunol., February 1, 2004; 172(3): 1727 - 1734.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
O. Rossier, S. R. Starkenburg, and N. P. Cianciotto
Legionella pneumophila Type II Protein Secretion Promotes Virulence in the A/J Mouse Model of Legionnaires' Disease Pneumonia
Infect. Immun., January 1, 2004; 72(1): 310 - 321.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Tateda, J. C. Deng, T. A. Moore, M. W. Newstead, R. Paine III, N. Kobayashi, K. Yamaguchi, and T. J. Standiford
Hyperoxia Mediates Acute Lung Injury and Increased Lethality in Murine Legionella Pneumonia: The Role of Apoptosis
J. Immunol., April 15, 2003; 170(8): 4209 - 4216.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
S. Aoki, Y. Hirakata, Y. Miyazaki, K. Izumikawa, K. Yanagihara, K. Tomono, Y. Yamada, T. Tashiro, S. Kohno, and S. Kamihira
Detection of Legionella DNA by PCR of whole-blood samples in a mouse model
J. Med. Microbiol., April 1, 2003; 52(4): 325 - 329.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Robey and N. P. Cianciotto
Legionella pneumophila feoAB Promotes Ferrous Iron Uptake and Intracellular Infection
Infect. Immun., October 1, 2002; 70(10): 5659 - 5669.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. M. Samrakandi, S. L. G. Cirillo, D. A. Ridenour, L. E. Bermudez, and J. D. Cirillo
Genetic and Phenotypic Differences between Legionella pneumophila Strains
J. Clin. Microbiol., April 1, 2002; 40(4): 1352 - 1362.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. C. Deng, K. Tateda, X. Zeng, and T. J. Standiford
Transient Transgenic Expression of Gamma Interferon Promotes Legionella pneumophila Clearance in Immunocompetent Hosts
Infect. Immun., October 1, 2001; 69(10): 6382 - 6390.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Robey, W. O'Connell, and N. P. Cianciotto
Identification of Legionella pneumophila rcp, a pagP-Like Gene That Confers Resistance to Cationic Antimicrobial Peptides and Promotes Intracellular Infection
Infect. Immun., July 1, 2001; 69(7): 4276 - 4286.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Salins, C. Newton, R. Widen, T. W. Klein, and H. Friedman
Differential Induction of Gamma Interferon in Legionella pneumophila- Infected Macrophages from BALB/c and A/J Mice
Infect. Immun., June 1, 2001; 69(6): 3605 - 3610.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Tateda, T. A. Moore, M. W. Newstead, W. C. Tsai, X. Zeng, J. C. Deng, G. Chen, R. Reddy, K. Yamaguchi, and T. J. Standiford
Chemokine-Dependent Neutrophil Recruitment in a Murine Model of Legionella Pneumonia: Potential Role of Neutrophils as Immunoregulatory Cells
Infect. Immun., April 1, 2001; 69(4): 2017 - 2024.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Tateda, T. A. Moore, J. C. Deng, M. W. Newstead, X. Zeng, A. Matsukawa, M. S. Swanson, K. Yamaguchi, and T. J. Standiford
Early Recruitment of Neutrophils Determines Subsequent T1/T2 Host Responses in a Murine Model of Legionella pneumophila Pneumonia
J. Immunol., March 1, 2001; 166(5): 3355 - 3361.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. L. G. Cirillo, L. E. Bermudez, S. H. El-Etr, G. E. Duhamel, and J. D. Cirillo
Legionella pneumophila Entry Gene rtxA Is Involved in Virulence
Infect. Immun., January 1, 2001; 69(1): 508 - 517.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. K. Brieland, C. Jackson, S. Hurst, D. Loebenberg, T. Muchamuel, R. Debets, R. Kastelein, T. Churakova, J. Abrams, R. Hare, et al.
Immunomodulatory Role of Endogenous Interleukin-18 in Gamma Interferon-Mediated Resolution of Replicative Legionella pneumophila Lung Infection
Infect. Immun., December 1, 2000; 68(12): 6567 - 6573.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
O. A. T. Alli, L.-Y. Gao, L. L. Pedersen, S. Zink, M. Radulic, M. Doric, and Y. Abu Kwaik
Temporal Pore Formation-Mediated Egress from Macrophages and Alveolar Epithelial Cells by Legionella pneumophila
Infect. Immun., November 1, 2000; 68(11): 6431 - 6440.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. K. Brieland, D. Loebenberg, F. Menzel, and R. S. Hare
Efficacy of SCH27899 in an Animal Model of Legionnaires' Disease Using Immunocompromised A/J Mice
Antimicrob. Agents Chemother., May 1, 2000; 44(5): 1333 - 1336.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
E. Diez, Z. Yaraghi, A. MacKenzie, and P. Gros
The Neuronal Apoptosis Inhibitory Protein (Naip) Is Expressed in Macrophages and Is Modulated After Phagocytosis and During Intracellular Infection with Legionella pneumophila
J. Immunol., February 1, 2000; 164(3): 1470 - 1477.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. D. Cirillo, S. L. G. Cirillo, L. Yan, L. E. Bermudez, S. Falkow, and L. S. Tompkins
Intracellular Growth in Acanthamoeba castellanii Affects Monocyte Entry Mechanisms and Enhances Virulence of Legionella pneumophila
Infect. Immun., September 1, 1999; 67(9): 4427 - 4434.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. K. Brieland, D. G. Remick, M. L. LeGendre, N. C. Engleberg, and J. C. Fantone
In Vivo Regulation of Replicative Legionella pneumophila Lung Infection by Endogenous Interleukin-12
Infect. Immun., January 1, 1998; 66(1): 65 - 69.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Susa, B. Ticac, T. Rukavina, M. Doric, and R. Marre
Legionella pneumophila Infection in Intratracheally Inoculated T Cell-Depleted or -Nondepleted A/J Mice
J. Immunol., January 1, 1998; 160(1): 316 - 321.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the American Society for Investigative Pathology.