This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kuroda, M. Articles by Machinami, R. Search for Related Content PubMed PubMed Citation Articles by Kuroda, M. Articles by Machinami, R.

American Journal of Pathology, Vol 147, 1221-1227, Copyright © 1995 by American Society for Investigative Pathology

REGULAR ARTICLES

Chimeric TLS/FUS-CHOP gene expression and the heterogeneity of its junction in human myxoid and round cell liposarcoma

M Kuroda, T Ishida, H Horiuchi, N Kida, H Uozaki, H Takeuchi, K Tsuji, T Imamura, S Mori and R Machinami
Department of Pathology, University of Tokyo, Japan.

Myxoid liposarcomas have a unique and specific t(12;16)q13;p11) chromosomal translocation. The breakpoint has recently been identified and shown to involve the TLS/FUS gene on chromosome 16 and the CHOP gene on chromosome 12. This translocation causes fusion of these genes resulting in the expression of a novel chimeric TLS/FUS-CHOP message. Using the polymerase chain reaction with primer sets derived from sequences of TLS/FUS and CHOP cDNAs, we could amplify three types of the fusion transcripts from seven of seven samples of myxoid and round cell liposarcomas. In six of the seven positive samples, two kinds of chimeric messenger RNAs were found that have been reported previously. However, the last sample had a novel chimeric message that had an extra sequence of 33 bp derived from the TLS/FUS gene. Thus, it was shown that these fusion transcripts had a varying extent of the sequence of TLS/FUS gene incorporated at the site of the fusion. However, the TLS/FUS-CHOP fusion transcripts were not detected in two pleomorphic liposarcomas or in three myxoid variants of malignant fibrous histiocytomas. Our findings indicate that in liposarcomas TLS/FUS-CHOP fusion transcripts have variations at the junction of chimeric messages, which was the case for Ewing's sarcoma. Detection of the chimeric message by reverse transcription polymerase chain reaction was also suggested to be a useful approach for the diagnosis of myxoid and round cell liposarcomas that have (12;16) translocation, and for distinguishing them from pleomorphic liposarcoma and myxoid variant of malignant fibrous histiocytomas.

This article has been cited by other articles:

				T Ohguri, M Hisaoka, S Kawauchi, K Sasaki, T Aoki, S Kanemitsu, A Matsuyama, Y Korogi, and H Hashimoto Cytogenetic analysis of myxoid liposarcoma and myxofibrosarcoma by array-based comparative genomic hybridisation J. Clin. Pathol., September 1, 2006; 59(9): 978 - 983. [Abstract] [Full Text] [PDF]

				U. Tateishi, T. Hasegawa, Y. Beppu, A. Kawai, M. Satake, and N. Moriyama Prognostic Significance of MRI Findings in Patients with Myxoid-Round Cell Liposarcoma Am. J. Roentgenol., March 1, 2004; 182(3): 725 - 731. [Abstract] [Full Text] [PDF]

				C. T. Storlazzi, F. Mertens, A. Nascimento, M. Isaksson, J. Wejde, O. Brosjo, N. Mandahl, and I. Panagopoulos Fusion of the FUS and BBF2H7 genes in low grade fibromyxoid sarcoma Hum. Mol. Genet., September 15, 2003; 12(18): 2349 - 2358. [Abstract] [Full Text] [PDF]

				U Tateishi, T Hasegawa, Y Beppu, A Kawai, and N Moriyama Prognostic significance of grading (MIB-1 system) in patients with myxoid liposarcoma J. Clin. Pathol., August 1, 2003; 56(8): 579 - 582. [Abstract] [Full Text] [PDF]

				T. Hosaka, Y. Nakashima, K. Kusuzaki, H. Murata, T. Nakayama, T. Nakamata, T. Aoyama, T. Okamoto, K. Nishijo, N. Araki, et al. A Novel Type of EWS-CHOP Fusion Gene in Two Cases of Myxoid Liposarcoma J. Mol. Diagn., August 1, 2002; 4(3): 164 - 171. [Abstract] [Full Text] [PDF]

				C. R. Antonescu, S. J. Tschernyavsky, R. Decuseara, D. H. Leung, J. M. Woodruff, M. F. Brennan, J. A. Bridge, J. R. Neff, J. R. Goldblum, and M. Ladanyi Prognostic Impact of P53 Status, TLS-CHOP Fusion Transcript Structure, and Histological Grade in Myxoid Liposarcoma: A Molecular and Clinicopathologic Study of 82 Cases Clin. Cancer Res., December 1, 2001; 7(12): 3977 - 3987. [Abstract] [Full Text] [PDF]

				C. R. Antonescu, A. Elahi, M. Humphrey, M. Y. Lui, J. H. Healey, M. F. Brennan, J. M. Woodruff, S. C. Jhanwar, and M. Ladanyi Specificity of TLS-CHOP Rearrangement for Classic Myxoid/Round Cell Liposarcoma: Absence in Predominantly Myxoid Well-Differentiated Liposarcomas J. Mol. Diagn., August 1, 2000; 2(3): 132 - 138. [Abstract] [Full Text]

				C. R. Antonescu, A. Elahi, J. H. Healey, M. F. Brennan, M. Y. Lui, J. Lewis, S. C. Jhanwar, J. M. Woodruff, and M. Ladanyi Monoclonality of Multifocal Myxoid Liposarcoma: Confirmation by Analysis of TLS-CHOP or EWS-CHOP Rearrangements Clin. Cancer Res., July 1, 2000; 6(7): 2788 - 2793. [Abstract] [Full Text]