This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by den Bakker, M. A. Articles by Zwarthoff, E. C. Search for Related Content PubMed PubMed Citation Articles by den Bakker, M. A. Articles by Zwarthoff, E. C.

American Journal of Pathology, Vol 147, 1339-1349, Copyright © 1995 by American Society for Investigative Pathology

REGULAR ARTICLES

Neurofibromatosis type 2 protein co-localizes with elements of the cytoskeleton

MA den Bakker, M Tascilar, PH Riegman, AC Hekman, W Boersma, PJ Janssen, TA de Jong, W Hendriks, TH van der Kwast and EC Zwarthoff
Department of Pathology, Erasmus University Rotterdam, The Netherlands.

The product of the neurofibromatosis type 2 (NF2) tumor suppressor gene is a 595-amino-acid protein bearing resemblance to a family of band-4.1- related proteins. These proteins, including ezrin, radixin, and moesin, probably function as molecular linking proteins, connecting the cytoskeleton to the cell membrane. On the grounds of the homology to the ezrin, radixin, and moesin proteins and on the basis of its predicted secondary structure, the NF2 protein is also thought to act as a cytoskeleton-cell membrane linking protein. Using monoclonal antibodies to amino- and carboxyl-terminal synthetic NF2 peptides we demonstrate the co-localization of the NF2 protein with elements of the cytoskeleton in a COS cell model system and in cultured human cells. Furthermore, the presence of the NF2 protein in tissue sections is shown. The monoclonal antibodies specifically stain smooth muscle cells and the stratum granulosum of the human epidermis. In cultured smooth muscle cells the NF2 protein co-localizes with actin stress fibers. Immunoelectron microscopy demonstrates the presence of the NF2 protein associated with keratohyalin granules and to a lesser extent with intermediate filaments in the human epidermis. We conclude that the NF2 protein is indeed associated with multiple elements of the cytoskeleton.

This article has been cited by other articles:

				A. I. McClatchey and M. Giovannini Membrane organization and tumorigenesis--the NF2 tumor suppressor, Merlin Genes & Dev., October 1, 2005; 19(19): 2265 - 2277. [Abstract] [Full Text] [PDF]

				K. Alfthan, L. Heiska, M. Gronholm, G. H. Renkema, and O. Carpen Cyclic AMP-dependent Protein Kinase Phosphorylates Merlin at Serine 518 Independently of p21-activated Kinase and Promotes Merlin-Ezrin Heterodimerization J. Biol. Chem., April 30, 2004; 279(18): 18559 - 18566. [Abstract] [Full Text] [PDF]

				J. T. Stickney, W. C. Bacon, M. Rojas, N. Ratner, and W. Ip Activation of the Tumor Suppressor Merlin Modulates Its Interaction with Lipid Rafts Cancer Res., April 15, 2004; 64(8): 2717 - 2724. [Abstract] [Full Text] [PDF]

				K. M. M. Schulze, C. O. Hanemann, H. W. Muller, and H. Hanenberg Transduction of wild-type merlin into human schwannoma cells decreases schwannoma cell growth and induces apoptosis Hum. Mol. Genet., January 1, 2002; 11(1): 69 - 76. [Abstract] [Full Text] [PDF]

				O.-M. Mykkanen, M. Gronholm, M. Ronty, M. Lalowski, P. Salmikangas, H. Suila, and O. Carpen Characterization of Human Palladin, a Microfilament-associated Protein Mol. Biol. Cell, October 1, 2001; 12(10): 3060 - 3073. [Abstract] [Full Text] [PDF]

				N. P. Hirsch, A. Murphy, and J. J. Radcliffe Neurofibromatosis: clinical presentations and anaesthetic implications Br. J. Anaesth., April 1, 2001; 86(4): 555 - 564. [Abstract] [Full Text] [PDF]

				L. Goutebroze, E. Brault, C. Muchardt, J. Camonis, and G. Thomas Cloning and Characterization of SCHIP-1, a Novel Protein Interacting Specifically with Spliced Isoforms and Naturally Occurring Mutant NF2 Proteins Mol. Cell. Biol., March 1, 2000; 20(5): 1699 - 1712. [Abstract] [Full Text]

				M. A. den Bakker, K. J. Vissers, A. C. Molijn, J. M. Kros, E. C. Zwarthoff, and T. H. van der Kwast Expression of the Neurofibromatosis Type 2 Gene in Human Tissues J. Histochem. Cytochem., November 1, 1999; 47(11): 1471 - 1480. [Abstract] [Full Text]

				M Sainio, F Zhao, L Heiska, O Turunen, M den Bakker, E Zwarthoff, M Lutchman, G. Rouleau, J Jaaskelainen, A Vaheri, et al. Neurofibromatosis 2 tumor suppressor protein colocalizes with ezrin and CD44 and associates with actin-containing cytoskeleton J. Cell Sci., January 9, 1997; 110(18): 2249 - 2260. [Abstract] [PDF]