This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kitamura, H. Articles by Ohkawa, H. Search for Related Content PubMed PubMed Citation Articles by Kitamura, H. Articles by Ohkawa, H.

American Journal of Pathology, Vol 147, 1682-1692, Copyright © 1995 by American Society for Investigative Pathology

REGULAR ARTICLES

Bone marrow neutrophilia and suppressed bone turnover in human interleukin-6 transgenic mice. A cellular relationship among hematopoietic cells, osteoblasts, and osteoclasts mediated by stromal cells in bone marrow

H Kitamura, H Kawata, F Takahashi, Y Higuchi, T Furuichi and H Ohkawa
Fuji-Gotemba Research Laboratory, Chugai Pharmaceutical Co., Shizuoka, Japan.

To elucidate the effect of interleukin-6 (IL-6) on bone and bone marrow (BM), human IL-6 transgenic mice (hIL-6 tgm) were produced. Their bone and BM were examined histologically, radiologically, histomorphometrically, and hematologically on a temporal basis. hIL-6 tgm showed histologically evident neutrophilia in BM. Increase in precursors of granulocytes and monocytes in hIL-6 tgm was demonstrated by an assay for colony forming unit in culture (CFU-C) of BM cells. Decrease in osteoblasts and osteoid and suppression of primary spongiosa formation were predominantly observed in hIL-6 tgm at 14 weeks old, the terminal stage of life for hIL-6 tgm. An assay for colony forming unit in fibroblastic (CFU-F) of BM cells revealed a decrease in osteoblast precursor (with regard to alkaline phosphatase- positive colonies) in hIL-6 tgm at 15 weeks old. Histomorphometry demonstrated a decrease of both osteoclast number and bone resorption in hIL-6 tgm. These results suggested that enhanced granulocytic hematopoiesis, suppressed bone turnover, and alteration of cellular population in stromal cells in BM occurred in hIL-6 tgm. Thus we provide new findings that facilitate understanding of cellular interrelationships among hematopoietic cells, osteoblasts, and osteoclasts mediated by stromal cells in BM.

This article has been cited by other articles:

				L. Duplomb, M. Baud'huin, C. Charrier, M. Berreur, V. Trichet, F. Blanchard, and D. Heymann Interleukin-6 Inhibits Receptor Activator of Nuclear Factor {kappa}B Ligand-Induced Osteoclastogenesis by Diverting Cells into the Macrophage Lineage: Key Role of Serine727 Phosphorylation of Signal Transducer and Activator of Transcription 3 Endocrinology, July 1, 2008; 149(7): 3688 - 3697. [Abstract] [Full Text] [PDF]

				F. Yoshitake, S. Itoh, H. Narita, K. Ishihara, and S. Ebisu Interleukin-6 Directly Inhibits Osteoclast Differentiation by Suppressing Receptor Activator of NF-{kappa}B Signaling Pathways J. Biol. Chem., April 25, 2008; 283(17): 11535 - 11540. [Abstract] [Full Text] [PDF]

				R. Chung, J. C. Cool, M. A. Scherer, B. K. Foster, and C. J. Xian Roles of neutrophil-mediated inflammatory response in the bony repair of injured growth plate cartilage in young rats J. Leukoc. Biol., December 1, 2006; 80(6): 1272 - 1280. [Abstract] [Full Text] [PDF]

				C. A. O'Brien, R. L. Jilka, Q. Fu, S. Stewart, R. S. Weinstein, and S. C. Manolagas IL-6 is not required for parathyroid hormone stimulation of RANKL expression, osteoclast formation, and bone loss in mice Am J Physiol Endocrinol Metab, November 1, 2005; 289(5): E784 - E793. [Abstract] [Full Text] [PDF]

				A. Teti New Concepts in Osteoclast Pathophysiology IBMS BoneKEy, June 1, 2005; 2(6): 17 - 22. [Full Text] [PDF]

				J. E. Compston Sex Steroids and Bone Physiol Rev, January 1, 2001; 81(1): 419 - 447. [Abstract] [Full Text] [PDF]

				V. A. Luyckx, B. Leclercq, L. K. Dowland, and A. S. L. Yu Diet-dependent hypercalciuria in transgenic mice with reduced CLC5 chloride channel expression PNAS, October 12, 1999; 96(21): 12174 - 12179. [Abstract] [Full Text] [PDF]

				H. Yoshitake, S. R. Rittling, D. T. Denhardt, and M. Noda Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorption PNAS, July 6, 1999; 96(14): 8156 - 8160. [Abstract] [Full Text] [PDF]

				T Laitala-Leinonen, C Lowik, S Papapoulos, and H. Vaananen Inhibition of intravacuolar acidification by antisense RNA decreases osteoclast differentiation and bone resorption in vitro J. Cell Sci., January 11, 1999; 112(21): 3657 - 3666. [Abstract] [PDF]

				R. Pacifici Editorial: Cytokines, Estrogen, and Postmenopausal Osteoporosis--The Second Decade Endocrinology, June 1, 1998; 139(6): 2659 - 2661. [Full Text] [PDF]

				K. Kawasaki, Y.-H. Gao, S. Yokose, Y. Kaji, T. Nakamura, T. Suda, K. Yoshida, T. Taga, T. Kishimoto, H. Kataoka, et al. Osteoclasts Are Present in gp130-Deficient Mice Endocrinology, November 1, 1997; 138(11): 4959 - 4965. [Abstract] [Full Text] [PDF]