| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 148, 225-232, Copyright © 1996 by American Society for Investigative Pathology
REGULAR ARTICLES |
AB Tuck, M Park, EE Sterns, A Boag and BE Elliott
Department of Pathology, Kingston General Hospital, Ontario, Canada.
Expression of hepatocyte growth factor (HGF) and HGF receptor (HGFR, product of the met proto-oncogene) mRNA were examined by nonisotopic in situ hybridization in a spectrum of benign and malignant human breast tissues. mRNA for both HGFR and HGF was detected in benign ductal epithelium. Epithelial expression of HGF mRNA was particularly intense in regions of ductal epithelial hyperplasia. Positive expression of HGF (but not HGFR) mRNA was also found in adipocytes, endothelial cells, and to varying degrees in stromal fibroblasts. In 12 of 12 cases of ductal carcinoma in situ and infiltrating ductal carcinoma, carcinoma cells showed a heterogeneous pattern of expression for both HGFR and HGF mRNA. In infiltrating ductal carcinomas, intense expression of HGFR mRNA was not restricted to ductular structures but as also seen in non- duct-forming carcinoma cells. The same zones of the tumors (most commonly at the advancing margins) that expressed strongly HGFR mRNA often were also strongly positive for HGF mRNA, suggesting a possible autocrine effect. The expression pattern of HGFR protein in 25 cases including the same series of tissues used for in situ hybridization analysis was similar to that of HGFR mRNA, as determined by an immunoperoxidase technique. The finding that HGFR is expressed by both benign and malignant epithelium, and its not restricted to duct-forming structures, suggests that, although the potential for HGF/HGFR binding is maintained in malignancy, the response to ligand binding at the level of the receptor or the cellular response to receptor activation may change at some point during progression.
This article has been cited by other articles:
 |
|
 |
|
  L. N. Bell, J. L. Ward, M. Degawa-Yamauchi, J. E. Bovenkerk, R. Jones, B. M. Cacucci, C. E. Gupta, C. Sheridan, K. Sheridan, S. S. Shankar, et al. Adipose tissue production of hepatocyte growth factor contributes to elevated serum HGF in obesity Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E843 - E848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Burgess, A. Coxon, S. Meyer, J. Sun, K. Rex, T. Tsuruda, Q. Chen, S.-Y. Ho, L. Li, S. Kaufman, et al. Fully Human Monoclonal Antibodies to Hepatocyte Growth Factor with Therapeutic Potential against Hepatocyte Growth Factor/c-Met-Dependent Human Tumors Cancer Res., February 1, 2006; 66(3): 1721 - 1729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Khoury, M. A. Naujokas, D. Zuo, V. Sangwan, M. M. Frigault, S. Petkiewicz, D. L. Dankort, W. J. Muller, and M. Park HGF Converts ErbB2/Neu Epithelial Morphogenesis to Cell Invasion Mol. Biol. Cell, February 1, 2005; 16(2): 550 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Tacchini, C. De Ponti, E. Matteucci, R. Follis, and M.A. Desiderio Hepatocyte growth factor-activated NF-{kappa}B regulates HIF-1 activity and ODC expression, implicated in survival, differently in different carcinoma cell lines Carcinogenesis, November 1, 2004; 25(11): 2089 - 2100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hov, R. U. Holt, T. B. Ro, U.-M. Fagerli, H. Hjorth-Hansen, V. Baykov, J. G. Christensen, A. Waage, A. Sundan, and M. Borset A Selective c-Met Inhibitor Blocks an Autocrine Hepatocyte Growth Factor Growth Loop in ANBL-6 Cells and Prevents Migration and Adhesion of Myeloma Cells Clin. Cancer Res., October 1, 2004; 10(19): 6686 - 6694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hecht, M. Papoutsi, H. D. Tran, J. Wilting, and L. Schweigerer Hepatocyte Growth Factor/c-Met Signaling Promotes the Progression of Experimental Human Neuroblastomas Cancer Res., September 1, 2004; 64(17): 6109 - 6118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-i. Nakashiro, S. Hara, Y. Shinohara, M. Oyasu, H. Kawamata, S. Shintani, H. Hamakawa, and R. Oyasu Phenotypic Switch from Paracrine to Autocrine Role of Hepatocyte Growth Factor in an Androgen-Independent Human Prostatic Carcinoma Cell Line, CWR22R Am. J. Pathol., August 1, 2004; 165(2): 533 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. G. Wright, J. Tsai, Z. Jia, and B. E. Elliott Inhibition by Copper(II) Binding of Hepatocyte Growth Factor (HGF) Interaction with Its Receptor Met and Blockade of HGF/Met Function J. Biol. Chem., July 30, 2004; 279(31): 32499 - 32506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Schag, S. M. Schmidt, M. R. Muller, T. Weinschenk, S. Appel, M. M. Weck, F. Grunebach, S. Stevanovic, H.-G. Rammensee, and P. Brossart Identification of C-Met Oncogene as a Broadly Expressed Tumor-Associated Antigen Recognized by Cytotoxic T-Lymphocytes Clin. Cancer Res., June 1, 2004; 10(11): 3658 - 3666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Parr, G. Watkins, R. E. Mansel, and W. G. Jiang The Hepatocyte Growth Factor Regulatory Factors in Human Breast Cancer Clin. Cancer Res., January 1, 2004; 10(1): 202 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Davol, R. Bagdasaryan, G. J. Elfenbein, A. L. Maizel, and A. R. Frackelton Jr. Shc Proteins Are Strong, Independent Prognostic Markers for Both Node-Negative and Node-Positive Primary Breast Cancer Cancer Res., October 15, 2003; 63(20): 6772 - 6783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Martin, C. Parr, G. Davies, G. Watkins, J. Lane, K. Matsumoto, T. Nakamura, R. E. Mansel, and W. G. Jiang Growth and angiogenesis of human breast cancer in a nude mouse tumour model is reduced by NK4, a HGF/SF antagonist Carcinogenesis, August 1, 2003; 24(8): 1317 - 1323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Popsueva, D. Poteryaev, E. Arighi, X. Meng, A. Angers-Loustau, D. Kaplan, M. Saarma, and H. Sariola GDNF promotes tubulogenesis of GFR{alpha}1-expressing MDCK cells by Src-mediated phosphorylation of Met receptor tyrosine kinase J. Cell Biol., April 14, 2003; 161(1): 119 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Imaizumi, H. Murota, S. Kanda, Y. Hishikawa, T. Koji, T. Taguchi, Y. Tanaka, Y. Yamada, S. Ikeda, T. Kohno, et al. Expression of the c-Met Proto-Oncogene and Its Possible Involvement in Liver Invasion in Adult T-cell Leukemia Clin. Cancer Res., January 1, 2003; 9(1): 181 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Vadnais, G. Nault, Z. Daher, M. Amraei, Y. Dodier, I. R. Nabi, and J. Noel Autocrine Activation of the Hepatocyte Growth Factor Receptor/Met Tyrosine Kinase Induces Tumor Cell Motility by Regulating Pseudopodial Protrusion J. Biol. Chem., December 6, 2002; 277(50): 48342 - 48350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lorenzato, M. Olivero, S. Patane, E. Rosso, A. Oliaro, P. M. Comoglio, and M. F. Di Renzo Novel Somatic Mutations of the MET Oncogene in Human Carcinoma Metastases Activating Cell Motility and Invasion Cancer Res., December 1, 2002; 62(23): 7025 - 7030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yu and G. Merlino Constitutive c-Met Signaling through a Nonautocrine Mechanism Promotes Metastasis in a Transgenic Transplantation Model Cancer Res., May 1, 2002; 62(10): 2951 - 2956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pediaditakis, S. P. S. Monga, W. M. Mars, and G. K. Michalopoulos Differential Mitogenic Effects of Single Chain Hepatocyte Growth Factor (HGF)/Scatter Factor and HGF/NK1 following Cleavage by Factor Xa J. Biol. Chem., April 12, 2002; 277(16): 14109 - 14115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. C. Hu, K. Y. Lam, S. Law, J. Wong, and G. Srivastava Profiling of Differentially Expressed Cancer-related Genes in Esophageal Squamous Cell Carcinoma (ESCC) Using Human Cancer cDNA Arrays: Overexpression of Oncogene MET Correlates with Tumor Differentiation in ESCC Clin. Cancer Res., November 1, 2001; 7(11): 3519 - 3525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Gmyrek, M. Walburg, C. P. Webb, H.-M. Yu, X. You, E. D. Vaughan, G. F. Vande Woude, and B. S. Knudsen Normal and Malignant Prostate Epithelial Cells Differ in Their Response to Hepatocyte Growth Factor/Scatter Factor Am. J. Pathol., August 1, 2001; 159(2): 579 - 590. [Abstract] [Full Text]
|
|
|
|
|
|
W. G. Jiang, D. Grimshaw, J. Lane, T. A. Martin, R. Abounder, J. Laterra, and R. E. Mansel A Hammerhead Ribozyme Suppresses Expression of Hepatocyte Growth Factor/Scatter Factor Receptor c-MET and Reduces Migration and Invasiveness of Breast Cancer Cells Clin. Cancer Res., August 1, 2001; 7(8): 2555 - 2562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shaharabany, R. Abramovitch, T. Kushnir, G. Tsarfaty, M. Ravid-Megido, J. Horev, R. Ron, Y. Itzchak, and I. Tsarfaty In Vivo Molecular Imaging of Met Tyrosine Kinase Growth Factor Receptor Activity in Normal Organs and Breast Tumors Cancer Res., June 1, 2001; 61(12): 4873 - 4878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tokunou, T. Niki, K. Eguchi, S. Iba, H. Tsuda, T. Yamada, Y. Matsuno, H. Kondo, Y. Saitoh, H. Imamura, et al. c-MET Expression in Myofibroblasts : Role in Autocrine Activation and Prognostic Significance in Lung Adenocarcinoma Am. J. Pathol., April 1, 2001; 158(4): 1451 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N.N.C. Tam, S.S.M. Chung, D.T.W. Lee, and Y.C. Wong Aberrant expression of hepatocyte growth factor and its receptor, c-Met, during sex hormone-induced prostatic carcinogenesis in the Noble rat Carcinogenesis, December 1, 2000; 21(12): 2183 - 2191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. J. M. Wielenga, R. van der Voort, T. E. I. Taher, L. Smit, E. A. Beuling, C. van Krimpen, M. Spaargaren, and S. T. Pals Expression of c-Met and Heparan-Sulfate Proteoglycan Forms of CD44 in Colorectal Cancer Am. J. Pathol., November 1, 2000; 157(5): 1563 - 1573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-i. Nakashiro, M. Okamoto, Y. Hayashi, and R. Oyasu Hepatocyte Growth Factor Secreted by Prostate-Derived Stromal Cells Stimulates Growth of Androgen-Independent Human Prostatic Carcinoma Cells Am. J. Pathol., September 1, 2000; 157(3): 795 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Favoni and A. De Cupis The Role of Polypeptide Growth Factors in Human Carcinomas: New Targets for a Novel Pharmacological Approach Pharmacol. Rev., June 1, 2000; 52(2): 179 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Otsuka, J. Jakubczak, W. Vieira, D. P. Bottaro, D. Breckenridge, W. J. Larochelle, and G. Merlino Disassociation of Met-Mediated Biological Responses In Vivo: the Natural Hepatocyte Growth Factor/Scatter Factor Splice Variant NK2 Antagonizes Growth but Facilitates Metastasis Mol. Cell. Biol., March 15, 2000; 20(6): 2055 - 2065. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Qiao, R. Saulnier, A. Patryzkat, N. Rahimi, L. Raptis, J. Rossiter, E. Tremblay, and B. Elliott Cooperative Effect of Hepatocyte Growth Factor and Fibronectin in Anchorage-independent Survival of Mammary Carcinoma Cells: Requirement for Phosphatidylinositol 3-Kinase Activity Cell Growth Differ., February 1, 2000; 11(2): 123 - 133. [Abstract] [Full Text]
|
|
|
|
|
|
R. van der Voort, T. E. I. Taher, V. J. M. Wielenga, M. Spaargaren, R. Prevo, L. Smit, G. David, G. Hartmann, E. Gherardi, and S. T. Pals Heparan Sulfate-modified CD44 Promotes Hepatocyte Growth Factor/Scatter Factor-induced Signal Transduction through the Receptor Tyrosine Kinase c-Met J. Biol. Chem., March 5, 1999; 274(10): 6499 - 6506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Stevenson, K. S. Ravichandran, and A. R. Frackelton Jr. Shc Dominant Negative Disrupts Cell Cycle Progression in Both G0-G1 and G2-M of ErbB2-positive Breast Cancer Cells Cell Growth Differ., January 1, 1999; 10(1): 61 - 71. [Abstract] [Full Text]
|
|
|
|
|
|
N. Rahimi, W. Hung, E. Tremblay, R. Saulnier, and B. Elliott c-Src Kinase Activity Is Required for Hepatocyte Growth Factor-induced Motility and Anchorage-independent Growth of Mammary Carcinoma Cells J. Biol. Chem., December 11, 1998; 273(50): 33714 - 33721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jeffers, M. Fiscella, C. P. Webb, M. Anver, S. Koochekpour, and G. F. Vande Woude The mutationally activated Met receptor mediates motility and metastasis PNAS, November 24, 1998; 95(24): 14417 - 14422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Adriaenssens, L. Dumont, S. Lottin, D. Bolle, A. Lepretre, A. Delobelle, F. Bouali, T. Dugimont, J. Coll, and J.-J. Curgy H19 Overexpression in Breast Adenocarcinoma Stromal Cells Is Associated with Tumor Values and Steroid Receptor Status but Independent of p53 and Ki-67 Expression Am. J. Pathol., November 1, 1998; 153(5): 1597 - 1607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lail-Trecker, R. Gulati, and J. J. Peluso A Role for Hepatocyte Growth Factors/Scatter Factor in Regulating Normal and Neoplastic Cells of Reproductive Tissues Reproductive Sciences, May 1, 1998; 5(3): 114 - 121. [Abstract] [PDF]
|
|
|
|
|
|
J. L. Jakubczak, W. J. Larochelle, and G. Merlino NK1, a Natural Splice Variant of Hepatocyte Growth Factor/Scatter Factor, Is a Partial Agonist In Vivo Mol. Cell. Biol., March 1, 1998; 18(3): 1275 - 1283. [Abstract] [Full Text]
|
|
|
|
|
|
H. Takayama, W. J. LaRochelle, R. Sharp, T. Otsuka, P. Kriebel, M. Anver, S. A. Aaronson, and G. Merlino Diverse tumorigenesis associated with aberrant development in mice overexpressing hepatocyte growth factor/scatter factor PNAS, January 21, 1997; 94(2): 701 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rusciano, P. Lorenzoni, and M. M. Burger Constitutive Activation of c-Met in Liver Metastatic B16 Melanoma Cells Depends on Both Substrate Adhesion and Cell Density and Is Regulated by a Cytosolic Tyrosine Phosphatase Activity J. Biol. Chem., August 23, 1996; 271(34): 20763 - 20769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Hung and B. Elliott Co-operative Effect of c-Src Tyrosine Kinase and Stat3 in Activation of Hepatocyte Growth Factor Expression in Mammary Carcinoma Cells J. Biol. Chem., April 6, 2001; 276(15): 12395 - 12403. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
