This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Garcia, J. H. Articles by Bree, M. P. Search for Related Content PubMed PubMed Citation Articles by Garcia, J. H. Articles by Bree, M. P.

American Journal of Pathology, Vol 148, 241-248, Copyright © 1996 by American Society for Investigative Pathology

REGULAR ARTICLES

Effects of CD11b/18 monoclonal antibody on rats with permanent middle cerebral artery occlusion

JH Garcia, KF Liu and MP Bree
Department of Pathology, Henry Ford Hospital, Detroit, Michigan 48202, USA.

The progression of a lesion from ischemic injury to infarct, after the permanent occlusion of a middle cerebral artery, may be influenced by the influx of leukocytes into the ischemic territory. We aimed to evaluate the effectiveness of treating rats that had permanent middle cerebral artery occlusion with a single dose of an anti-CD11b/18 monoclonal antibody injected 1 hour after the arterial occlusion. To mimic the clinical situation of patients with ischemic strokes who may be treated within 1 hour of the ischemic event, the artery remained occluded. Forty-one adult Wistar rats had permanent middle cerebral artery occlusion, and one was subjected to a sham operation. One hour later, 22 rats received CD11b/18 monoclonal antibody and an additional 20 were injected either with a nonspecific antibody (n = 10) or a buffer solution (n = 10). Experiments were terminated at intervals ranging 12 to 96 hours after the arterial occlusion. Endpoints included neurological testing, daily evaluation of body weight, counts of white blood cells in the peripheral blood, measurement of the area of pallor in the ischemic hemisphere, counts of necrotic neurons, and counts of leukocytes sequestered in the ischemic hemisphere. In experiments terminated 12 hours after the arterial occlusion (n = 4), there were fewer necrotic neurons in the group treated with the CD11b/18 monoclonal antibody compared with the two controls (P < .05), but this difference was not reflected in the neurological scores. Numbers of necrotic neurons in experiments terminated > 12 hours later were not different among the three subgroups. White blood cell counts in peripheral blood were lower in animals with arterial occlusion injected with the monoclonal antibody CD11b/18 (P < .05); numbers of leukocytes sequestered in the ischemic hemisphere were not different in the three groups. Neither changes in body weight nor in the volume of the area of pallor were significantly different among the three groups.

This article has been cited by other articles:

				S. G. Soriano, A. Coxon, Y. F. Wang, M. P. Frosch, S. A. Lipton, P. R. Hickey, T. N. Mayadas, and P. H. Chan Mice Deficient in Mac-1 (CD11b/CD18) Are Less Susceptible to Cerebral Ischemia/Reperfusion Injury • Editorial Comment Stroke, January 1, 1999; 30(1): 134 - 139. [Abstract] [Full Text] [PDF]

				L. Pantoni, C. Sarti, and D. Inzitari Cytokines and Cell Adhesion Molecules in Cerebral Ischemia : Experimental Bases and Therapeutic Perspectives Arterioscler. Thromb. Vasc. Biol., April 1, 1998; 18(4): 503 - 513. [Abstract] [Full Text] [PDF]

				L. Pantoni, L. Bartolini, G. Pracucci, D. Inzitari, and J. H. Garcia Interrater Agreement on a Simple Neurological Score in Rats • Response Stroke, April 1, 1998; 29(4): 871 - 872. [Full Text] [PDF]

				M. Schroeter, S. Jander, I. Huitinga, O. W. Witte, G. Stoll, and W. D. Dietrich Phagocytic Response in Photochemically Induced Infarction of Rat Cerebral Cortex: The Role of Resident Microglia Stroke, February 1, 1997; 28(2): 382 - 386. [Abstract] [Full Text]