help button home button Am J Pathol ASIP MEMBERSHIP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yaremko, M. L.
Right arrow Articles by Westbrook, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yaremko, M. L.
Right arrow Articles by Westbrook, C. A.

American Journal of Pathology, Vol 148, 95-104, Copyright © 1996 by American Society for Investigative Pathology

REGULAR ARTICLES

Immunomagnetic separation can enrich fixed solid tumors for epithelial cells

ML Yaremko, PR Kelemen, C Kutza, D Barker and CA Westbrook
Department of Pathology, University of Chicago, Illinois 60637, USA.

Immunomagnetic separation is a highly specific technique for the enrichment or isolation of cells from a variety of fresh tissues and microorganisms or molecules from suspensions. Because new techniques for molecular analysis of solid tumors are now applicable to fixed tissue but sometimes require or benefit from enrichment for tumor cells, we tested the efficacy of immunomagnetic separation for enriching fixed solid tumors for malignant epithelial cells. We applied it to two different tumors and fixation methods to separate neoplastic from non-neoplastic cells in primary colorectal cancers and metastatic breast cancers, and were able to enrich to a high degree of purity. Immunomagnetic separation was effective in unembedded fixed tissue as well as fixed paraffin-embedded tissue. The magnetically separated cells were amenable to fluorescence in situ hybridization and polymerase chain reaction amplification of their DNA with minimal additional manipulation. The high degree of enrichment achieved before amplification contributed to interpretation of loss of heterozygosity in metastatic breast cancers, and simplified fluorescence in situ hybridization analysis because only neoplastic cells were hybridized and counted. Immunomagnetic separation is effective for the enrichment of fixed solid tumors, can be performed with widely available commercial antibodies, and requires little specialized instrumentation. It can contribute to interpretation of results in situations where enrichment by other methods is difficult or not possible.


This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
B. Molnar, A. Ladanyi, L. Tanko, L. Sreter, and Z. Tulassay
Circulating Tumor Cell Clusters in the Peripheral Blood of Colorectal Cancer Patients
Clin. Cancer Res., December 1, 2001; 7(12): 4080 - 4085.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. C. Jamur, A. C. G. Grodzki, A. N. Moreno, W. D. Swaim, R. P. Siraganian, and C. Oliver
Immunomagnetic Isolation of Rat Bone Marrow-derived and Peritoneal Mast Cells
J. Histochem. Cytochem., December 1, 1997; 45(12): 1715 - 1722.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the American Society for Investigative Pathology.