This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ricevuto, E. Articles by Martinotti, S. Search for Related Content PubMed PubMed Citation Articles by Ricevuto, E. Articles by Martinotti, S.

American Journal of Pathology, Vol 148, 405-413, Copyright © 1996 by American Society for Investigative Pathology

REGULAR ARTICLES

Molecular diagnosis of p53 mutations in gastric carcinoma by touch preparation

E Ricevuto, C Ficorella, C Fusco, K Cannita, A Tessitore, E Toniato, A Gabriele, L Frati, P Marchetti, A Gulino and S Martinotti
Department of Experimental Medicine, University of L'Aquila, Italy.

Thirty-one tumor samples from selected cases of gastric carcinoma were analyzed for mutations of the p53 tumor suppressor gene. Template DNA was prepared according to the touch preparation procedure, which allowed us to isolate clusters of neoplastic cells out of a stromal cellular background to be used as a template in the amplification of target exons of the p53 locus. In our present study, by polymerase chain reaction/single strand conformation polymorphism analysis we give evidence of p53 mutations occurring in the DNA-binding core domain of the protein (exons 5 through 9), which are clustered in stages III and IV of the disease (six mutations out of seventeen samples; 35%). No p53 mutations were detected in fourteen gastric cancer samples at I and II stages. Beside the use of conventional molecular scanning procedures, our study proposes the application of the touch preparation method to increase the detection of genetic alterations in human solid tumors.

This article has been cited by other articles:

				A. Shibata, J. Parsonnet, T. A. Longacre, M. I. Garcia, B. Puligandla, R.E. Davis, J. H. Vogelman, N. Orentreich, and L. A. Habel CagA status of Helicobacter pylori infection and p53 gene mutations in gastric adenocarcinoma Carcinogenesis, March 1, 2002; 23(3): 419 - 424. [Abstract] [Full Text] [PDF]

				A. Calogero, A. Arcella, G. De Gregorio, A. Porcellini, D. Mercola, C. Liu, V. Lombari, M. Zani, G. Giannini, F. M. Gagliardi, et al. The Early Growth Response Gene EGR-1 Behaves as a Suppressor Gene That Is Down-Regulated Independent of ARF/Mdm2 but not p53 Alterations in Fresh Human Gliomas Clin. Cancer Res., September 1, 2001; 7(9): 2788 - 2796. [Abstract] [Full Text] [PDF]