This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nikkari, S. T. Articles by Clowes, A. W. Search for Related Content PubMed PubMed Citation Articles by Nikkari, S. T. Articles by Clowes, A. W.

American Journal of Pathology, Vol 148, 777-783, Copyright © 1996 by American Society for Investigative Pathology

REGULAR ARTICLES

Expression of collagen, interstitial collagenase, and tissue inhibitor of metalloproteinases-1 in restenosis after carotid endarterectomy

ST Nikkari, RL Geary, T Hatsukami, M Ferguson, R Forough, CE Alpers and AW Clowes
Department of Surgery, University of Washington, Seattle 98195-6410, USA.

Extracellular matrix is the principal component of the fibrous caps of atherosclerotic plaques and intimal hyperplastic lesions of reconstructed arteries. Interstitial collagen form an important part of the matrix, and the balance between collagen synthesis and degradation by interstitial collagenase (matrix metalloproteinase-1, MMP-1) may determine whether plaques rupture or vessels develop stenosis. We examined type I procollagen gene expression in human atherosclerotic and restenotic carotid arteries using in situ messenger RNA (mRNA) hybridization and the expression of MMP-1 and its endogenous inhibitor (tissue inhibitor of metalloproteinases-1, TIMP-1) by immunohistochemistry. Compared with normal arteries, atherosclerotic plaques bed increased expression of immunoreactive MMP-1 and TIMP-1 with modest increase of type 1 procollagen mRNA. Early restenotic lesions (< 1.5 years) contained abundant type I procollagen mRNA but little immunoreactive MMP-1 and TIMP-1. Late restenotic lesions (> 4 years) resembled atheroma and exhibited increased immunoreactive MMP-1 and TIMP-1 as well as abundant type I procollagen mRNA. Compared with atherosclerotic plaques, type I procollagen is increased and MMP-1 is decreased in early restenotic lesions. MMP-1 and TIMP-1 expressions are upregulated in lesions with a clear atheroma. These findings suggest that the balance between proteolysis and matrix synthesis may influence both the stability of atheromatous plaques and the development of restenotic lesions.

This article has been cited by other articles:

				X. Qin, M. A. Corriere, L. M. Matrisian, and R. J. Guzman Matrix Metalloproteinase Inhibition Attenuates Aortic Calcification Arterioscler. Thromb. Vasc. Biol., July 1, 2006; 26(7): 1510 - 1516. [Abstract] [Full Text] [PDF]

				G. T. Jones, I. P. Kay, J.W. S. Chu, G.T. Wilkins, L.V. Phillips, M. McCormick, A.M. van Rij, and M.J.A. Williams Elevated Plasma Active Matrix Metalloproteinase-9 Level Is Associated With Coronary Artery In-Stent Restenosis Arterioscler. Thromb. Vasc. Biol., July 1, 2006; 26(7): e121 - e125. [Abstract] [Full Text] [PDF]

				A. C. Newby Matrix metalloproteinases regulate migration, proliferation, and death of vascular smooth muscle cells by degrading matrix and non-matrix substrates Cardiovasc Res, February 15, 2006; 69(3): 614 - 624. [Abstract] [Full Text] [PDF]

				A. C. Newby Dual Role of Matrix Metalloproteinases (Matrixins) in Intimal Thickening and Atherosclerotic Plaque Rupture Physiol Rev, January 1, 2005; 85(1): 1 - 31. [Abstract] [Full Text] [PDF]

				Z. Luan, A. J. Chase, and A. C. Newby Statins Inhibit Secretion of Metalloproteinases-1, -2, -3, and -9 From Vascular Smooth Muscle Cells and Macrophages Arterioscler. Thromb. Vasc. Biol., May 1, 2003; 23(5): 769 - 775. [Abstract] [Full Text] [PDF]

				J.-L. Beaudeux, L. Burc, F. Imbert-Bismut, P. Giral, M. Bernard, E. Bruckert, and M. J. Chapman Serum Plasma Pregnancy-Associated Protein A: A Potential Marker of Echogenic Carotid Atherosclerotic Plaques in Asymptomatic Hyperlipidemic Subjects at High Cardiovascular Risk Arterioscler. Thromb. Vasc. Biol., January 1, 2003; 23(1): e7 - 10. [Abstract] [Full Text] [PDF]

				R. L. Geary, J. M. Wong, A. Rossini, S. M. Schwartz, and L. D. Adams Expression Profiling Identifies 147 Genes Contributing to a Unique Primate Neointimal Smooth Muscle Cell Phenotype Arterioscler. Thromb. Vasc. Biol., December 1, 2002; 22(12): 2010 - 2016. [Abstract] [Full Text] [PDF]

				M. Bailey, H. Xiao, M. Ogle, and N. Vyavahare Aluminum Chloride Pretreatment of Elastin Inhibits Elastolysis by Matrix Metalloproteinases and Leads to Inhibition of Elastin-Oriented Calcification Am. J. Pathol., December 1, 2001; 159(6): 1981 - 1986. [Abstract] [Full Text] [PDF]

				N. Vyavahare, P. L. Jones, S. Tallapragada, and R. J. Levy Inhibition of Matrix Metalloproteinase Activity Attenuates Tenascin-C Production and Calcification of Implanted Purified Elastin in Rats Am. J. Pathol., September 1, 2000; 157(3): 885 - 893. [Abstract] [Full Text] [PDF]

				S. Jormsjo, S. Ye, J. Moritz, D. H. Walter, S. Dimmeler, A. M. Zeiher, A. Henney, A. Hamsten, and P. Eriksson Allele-Specific Regulation of Matrix Metalloproteinase-12 Gene Activity Is Associated With Coronary Artery Luminal Dimensions in Diabetic Patients With Manifest Coronary Artery Disease Circ. Res., May 12, 2000; 86(9): 998 - 1003. [Abstract] [Full Text] [PDF]

				A. B. Zaltsman, S. J. George, and A. C. Newby Increased Secretion of Tissue Inhibitors of Metalloproteinases 1 and 2 From the Aortas of Cholesterol Fed Rabbits Partially Counterbalances Increased Metalloproteinase Activity Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1700 - 1707. [Abstract] [Full Text] [PDF]

				G. Plenz, A. Dorszewski, G. Breithardt, and H. Robenek Expression of Type VIII Collagen After Cholesterol Diet and Injury in the Rabbit Model of Atherosclerosis Arterioscler. Thromb. Vasc. Biol., May 1, 1999; 19(5): 1201 - 1209. [Abstract] [Full Text] [PDF]

				A. Kranzhofer, A. H. Baker, S. J. George, and A. C. Newby Expression of Tissue Inhibitor of Metalloproteinase-1, -2, and -3 During Neointima Formation in Organ Cultures of Human Saphenous Vein Arterioscler. Thromb. Vasc. Biol., February 1, 1999; 19(2): 255 - 265. [Abstract] [Full Text] [PDF]

				M. D. Rekhter Collagen synthesis in atherosclerosis: too much and not enough Cardiovasc Res, February 1, 1999; 41(2): 376 - 384. [Abstract] [Full Text] [PDF]

				R. P. Fabunmi, G. K. Sukhova, S. Sugiyama, and P. Libby Expression of Tissue Inhibitor of Metalloproteinases-3 in Human Atheroma and Regulation in Lesion-Associated Cells : A Potential Protective Mechanism in Plaque Stability Circ. Res., August 10, 1998; 83(3): 270 - 278. [Abstract] [Full Text] [PDF]

				E. Allaire, D. Hasenstab, R. D. Kenagy, B. Starcher, M. M. Clowes, and A. W. Clowes Prevention of Aneurysm Development and Rupture by Local Overexpression of Plasminogen Activator Inhibitor-1 Circulation, July 21, 1998; 98(3): 249 - 255. [Abstract] [Full Text] [PDF]

				S. Li, L. H. Chow, and J. G. Pickering Cell Surface-bound Collagenase-1 and Focal Substrate Degradation Stimulate the Rear Release of Motile Vascular Smooth Muscle Cells J. Biol. Chem., November 3, 2000; 275(45): 35384 - 35392. [Abstract] [Full Text] [PDF]