This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, C. Articles by Tammi, R. Search for Related Content PubMed PubMed Citation Articles by Wang, C. Articles by Tammi, R.

American Journal of Pathology, Vol 148, 1861-1869, Copyright © 1996 by American Society for Investigative Pathology

REGULAR ARTICLES

Hyaluronan distribution in the normal epithelium of esophagus, stomach, and colon and their cancers

C Wang, M Tammi, H Guo and R Tammi
Department of Anatomy, University of Kuopio, Finland.

The distribution of hyaluronan (HA) in normal gastrointestinal wall and in tumors originating from their epithelium was studied using a specific probe prepared from cartilage proteoglycan (bHABC, biotinylated hyaluronan binding complex). The normal stratified squamous epithelium of esophagus showed an intense HA staining in the basal and lower intermediate layers, whereas the simple epithelia in the stomach and large intestine were HA negative. Esophageal in situ carcinomas expressed HA also in the cell layers close to the luminal surface, in regions normally negative. Most of the invasive squamous cell carcinomas maintained their HA expression, but in very poorly differentiated types the tumor parenchyma was devoid of HA. In both gastric and colonic adenocarcinomas the tumor parenchyma showed no HA. The stromal tissue was intensely HA positive in all tumors. Cancer cells invading the intestinal smooth muscle were surrounded by copious amounts of HA, whereas the muscular layer was otherwise very poor in HA staining. These results show that relatively well differentiated carcinoma cells themselves retain the high or low HA expression pattern of their original epithelium, whereas tumors stimulate HA deposition in the surrounding stroma.

This article has been cited by other articles:

				G. Ishii, T.-K. Ito, K. Aoyagi, H. Fujimoto, H. Chiba, T. Hasebe, S. Fujii, K. Nagai, H. Sasaki, and A. Ochiai Presence of Human Circulating Progenitor Cells for Cancer Stromal Fibroblasts in the Blood of Lung Cancer Patients Stem Cells, June 1, 2007; 25(6): 1469 - 1477. [Abstract] [Full Text] [PDF]

				A. E. Ducale, S. I. Ward, T. Dechert, and D. R. Yager Regulation of hyaluronan synthase-2 expression in human intestinal mesenchymal cells: mechanisms of interleukin-1{beta}-mediated induction Am J Physiol Gastrointest Liver Physiol, September 1, 2005; 289(3): G462 - G470. [Abstract] [Full Text] [PDF]

				M. Edward, C. Gillan, D. Micha, and R.H. Tammi Tumour regulation of fibroblast hyaluronan expression: a mechanism to facilitate tumour growth and invasion Carcinogenesis, July 1, 2005; 26(7): 1215 - 1223. [Abstract] [Full Text] [PDF]

				C. D. Blundell, P. L. DeAngelis, A. J. Day, and A. Almond Use of 15N-NMR to resolve molecular details in isotopically-enriched carbohydrates: sequence-specific observations in hyaluronan oligomers up to decasaccharides Glycobiology, November 1, 2004; 14(11): 999 - 1009. [Abstract] [Full Text] [PDF]

				H. Zhang, S. L. Baader, M. Sixt, J. Kappler, and U. Rauch Neurocan-GFP Fusion Protein: A New Approach to Detect Hyaluronan on Tissue Sections and Living Cells J. Histochem. Cytochem., July 1, 2004; 52(7): 915 - 922. [Abstract] [Full Text] [PDF]

				H.-R. Kim, M. A. Wheeler, C. M. Wilson, J. Iida, D. Eng, M. A. Simpson, J. B. McCarthy, and K. M. Bullard Hyaluronan Facilitates Invasion of Colon Carcinoma Cells in Vitro via Interaction with CD44 Cancer Res., July 1, 2004; 64(13): 4569 - 4576. [Abstract] [Full Text] [PDF]

				S. Sillanpaa, M. A. Anttila, K. Voutilainen, R. H. Tammi, M. I. Tammi, S. V. Saarikoski, and V.-M. Kosma CD44 Expression Indicates Favorable Prognosis in Epithelial Ovarian Cancer Clin. Cancer Res., November 1, 2003; 9(14): 5318 - 5324. [Abstract] [Full Text] [PDF]

				E. L. J. Hiltunen, M. Anttila, A. Kultti, K. Ropponen, J. Penttinen, M. Yliskoski, A. T. Kuronen, M. Juhola, R. Tammi, M. Tammi, et al. Elevated Hyaluronan Concentration without Hyaluronidase Activation in Malignant Epithelial Ovarian Tumors Cancer Res., November 15, 2002; 62(22): 6410 - 6413. [Abstract] [Full Text] [PDF]

				J. M. Karjalainen, R. H. Tammi, M. I. Tammi, M. J. Eskelinen, U. M. Agren, J. J. Parkkinen, E. M. Alhava, and V.-M. Kosma Reduced Level of CD44 and Hyaluronan Associated with Unfavorable Prognosis in Clinical Stage I Cutaneous Melanoma Am. J. Pathol., September 1, 2000; 157(3): 957 - 965. [Abstract] [Full Text] [PDF]

				P. Auvinen, R. Tammi, J. Parkkinen, M. Tammi, U. Agren, R. Johansson, P. Hirvikoski, M. Eskelinen, and V.-M. Kosma Hyaluronan in Peritumoral Stroma and Malignant Cells Associates with Breast Cancer Spreading and Predicts Survival Am. J. Pathol., February 1, 2000; 156(2): 529 - 536. [Abstract] [Full Text] [PDF]

				M. A. Anttila, R. H. Tammi, M. I. Tammi, K. J. Syrjänen, S. V. Saarikoski, and V.-M. Kosma High Levels of Stromal Hyaluronan Predict Poor Disease Outcome in Epithelial Ovarian Cancer Cancer Res., January 1, 2000; 60(1): 150 - 155. [Abstract] [Full Text]

				V. B. Lokeshwar, M. J. Young, G. Goudarzi, N. Iida, A. I. Yudin, G. N. Cherr, and M. G. Selzer Identification of Bladder Tumor-derived Hyaluronidase: Its Similarity to HYAL1 Cancer Res., September 1, 1999; 59(17): 4464 - 4470. [Abstract] [Full Text] [PDF]

				R. Tammi, D. MacCallum, V. C. Hascall, J.-P. Pienimaki, M. Hyttinen, and M. Tammi Hyaluronan Bound to CD44 on Keratinocytes Is Displaced by Hyaluronan Decasaccharides and Not Hexasaccharides J. Biol. Chem., October 30, 1998; 273(44): 28878 - 28888. [Abstract] [Full Text] [PDF]

				V. B. Lokeshwar and M. G. Selzer Differences in Hyaluronic Acid-mediated Functions and Signaling in Arterial, Microvessel, and Vein-derived Human Endothelial Cells J. Biol. Chem., September 1, 2000; 275(36): 27641 - 27649. [Abstract] [Full Text] [PDF]

				J.-P. Pienimaki, K. Rilla, C. Fulop, R. K. Sironen, S. Karvinen, S. Pasonen, M. J. Lammi, R. Tammi, V. C. Hascall, and M. I. Tammi Epidermal Growth Factor Activates Hyaluronan Synthase 2 in Epidermal Keratinocytes and Increases Pericellular and Intracellular Hyaluronan J. Biol. Chem., June 1, 2001; 276(23): 20428 - 20435. [Abstract] [Full Text] [PDF]

				V. B. Lokeshwar, D. Rubinowicz, G. L. Schroeder, E. Forgacs, J. D. Minna, N. L. Block, M. Nadji, and B. L. Lokeshwar Stromal and Epithelial Expression of Tumor Markers Hyaluronic Acid and HYAL1 Hyaluronidase in Prostate Cancer J. Biol. Chem., April 6, 2001; 276(15): 11922 - 11932. [Abstract] [Full Text] [PDF]