This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bussolati, G. Articles by Sapino, A. Search for Related Content PubMed PubMed Citation Articles by Bussolati, G. Articles by Sapino, A.

American Journal of Pathology, Vol 148, 1895-1903, Copyright © 1996 by American Society for Investigative Pathology

REGULAR ARTICLES

Immunolocalization and gene expression of oxytocin receptors in carcinomas and non-neoplastic tissues of the breast

G Bussolati, P Cassoni, G Ghisolfi, F Negro and A Sapino
Department of Biomedical Sciences and Oncology, University of Torino, Torino, Italy.

Recent evidence indicates that oxytocin (OT), in addition to the induction of myometrial and myoepithelial cell contraction, can influence proliferation and differentiation in developing mammary glands and in breast cancer cells, hence the interest in detecting and locating OT receptors (OTRs). We produced rabbit antisera and a monoclonal antibody against a synthetic peptide corresponding to the carboxy terminus of the predicted OTR sequence. We tested their specificity in immunoblasts and immunocytochemical tests. All of the antibodies specifically stained myometrium (at term of pregnancy). In the human breast, OTRs were detected in myoepithelial cells along ducts of normal lobules and in sclerosing adenosis. Intraductal cells in benign hyperplastic lesions were also positive. OTRs were demonstrated in cases of primary and metastatic carcinomas of the breast. In the same tissues, OTR gene expression was shown by reverse transcriptase polymerase chain reaction procedures detecting the specific mRNA. These results suggest that the interaction between OT and its receptors might play a role in the origin and evolution of non-neoplastic lesions and carcinomas of the breast.

This article has been cited by other articles:

				H. Thackare, H. D. Nicholson, and K. Whittington Oxytocin--its role in male reproduction and new potential therapeutic uses Hum. Reprod. Update, July 1, 2006; 12(4): 437 - 448. [Abstract] [Full Text] [PDF]

				P. Cassoni, T. Marrocco, B. Bussolati, E. Allia, L. Munaron, A. Sapino, and G. Bussolati Oxytocin Induces Proliferation and Migration in Immortalized Human Dermal Microvascular Endothelial Cells and Human Breast Tumor-Derived Endothelial Cells Mol. Cancer Res., June 1, 2006; 4(6): 351 - 359. [Abstract] [Full Text] [PDF]

				V. Lollivier, P.-G. Marnet, S. Delpal, D. Rainteau, C. Achard, A. Rabot, and M. Ollivier-Bousquet Oxytocin stimulates secretory processes in lactating rabbit mammary epithelial cells J. Physiol., January 1, 2006; 570(1): 125 - 140. [Abstract] [Full Text] [PDF]

				J. C. Reubi Peptide Receptors as Molecular Targets for Cancer Diagnosis and Therapy Endocr. Rev., August 1, 2003; 24(4): 389 - 427. [Abstract] [Full Text] [PDF]

				C. Pequeux, C. Breton, J.-C. Hendrick, M.-T. Hagelstein, H. Martens, R. Winkler, V. Geenen, and J.-J. Legros Oxytocin Synthesis and Oxytocin Receptor Expression by Cell Lines of Human Small Cell Carcinoma of the Lung Stimulate Tumor Growth through Autocrine/Paracrine Signaling Cancer Res., August 15, 2002; 62(16): 4623 - 4629. [Abstract] [Full Text] [PDF]

				P. Cassoni, A. Sapino, S. Deaglio, B. Bussolati, M. Volante, L. Munaron, A. Albini, A. Torrisi, and G. Bussolati Oxytocin Is a Growth Factor for Kaposi's Sarcoma Cells: Evidence of Endocrine-Immunological Cross-Talk Cancer Res., April 1, 2002; 62(8): 2406 - 2413. [Abstract] [Full Text] [PDF]

				G. Bussolati, M. Chinol, B. Chini, A. Nacca, P. Cassoni, and G. Paganelli 111In-labeled 1,4,7,10-Tetraazacyclododecane-N,N',N'',N'''-tetraacetic Acid-Lys8-Vasotocin: A New Powerful Radioligand for Oxytocin Receptor-expressing Tumors Cancer Res., June 1, 2001; 61(11): 4393 - 4397. [Abstract] [Full Text] [PDF]

				G. Bussolati and P. Cassoni Editorial: The Oxytocin/Oxytocin Receptor System--Expect the Unexpected Endocrinology, April 1, 2001; 142(4): 1377 - 1379. [Full Text]

				P. Cassoni, A. Sapino, L. Munaron, S. Deaglio, B. Chini, A. Graziani, A. Ahmed, and G. Bussolati Activation of Functional Oxytocin Receptors Stimulates Cell Proliferation in Human Trophoblast and Choriocarcinoma Cell Lines Endocrinology, March 1, 2001; 142(3): 1130 - 1136. [Abstract] [Full Text] [PDF]

				J.-Y. Cho, R. Léveillé, R. Kao, B. Rousset, A. F. Parlow, W. E. Burak Jr., E. L. Mazzaferri, and S. M. Jhiang Hormonal Regulation of Radioiodide Uptake Activity and Na+/I- Symporter Expression in Mammary Glands J. Clin. Endocrinol. Metab., August 1, 2000; 85(8): 2936 - 2943. [Abstract] [Full Text]

				J. F. Novak, M. B. Judkins, M. I. Chernin, P. Cassoni, G. Bussolati, J. A. Nitche, and S. K. Nishimoto A Plasmin-derived Hexapeptide from the Carboxyl End of Osteocalcin Counteracts Oxytocin-mediated Growth of Inhibition of Osteosarcoma Cells Cancer Res., July 1, 2000; 60(13): 3470 - 3476. [Abstract] [Full Text]

				J. A. Copland, Y.-J. Jeng, Z. Strakova, K. L. Ives, M. R. Hellmich, and M. S. Soloff Demonstration of Functional Oxytocin Receptors in Human Breast Hs578T Cells and Their Up-Regulation through a Protein Kinase C-Dependent Pathway Endocrinology, May 1, 1999; 140(5): 2258 - 2267. [Abstract] [Full Text]