This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weintraub, A. S. Articles by Taubman, M. B. Search for Related Content PubMed PubMed Citation Articles by Weintraub, A. S. Articles by Taubman, M. B.

American Journal of Pathology, Vol 149, 259-272, Copyright © 1996 by American Society for Investigative Pathology

REGULAR ARTICLES

Autocrine secretion of osteopontin by vascular smooth muscle cells regulates their adhesion to collagen gels

AS Weintraub, CM Giachelli, RS Krauss, M Almeida and MB Taubman
Department of Pediatrics, Mount Sinai School of Medicine, New York, New York, USA.

Osteopontin (OPN) is a secreted protein postulated to facilitate vascular smooth muscle cell (VSMC) adhesion and migration. Rat aortic VSMC lines were isolated after infection with recombinant retroviruses harboring OPN sense and antisense constructs. All lines grew normally in monolayer culture. On three-dimensional collagen gels, normal VSMCs and lines containing sense constructs (n=15) or empty vector (n=10) attached to gel and invaded the matrix. Four of five antisense clones did not adhere or invade. Antisense clones had lower OPN levels after stimulation with angiotensin II than sense clones or clones containing the empty vector (antisense, 257+/-102 ng/ml; sense, 473+/-104; vector, 434+/-66). Non-adhering antisense clones had lower mean OPN levels after angiotensin II stimulation (161+/-47 ng/ml) than sense or antisense lines with normal adhesion (486+/-63 ng/ml). The ability to adhere correlated with OPN levels >250 ng/ml. Adhesion and invasion were fully restored with addition of 100 to 200 ng/ml of exogenous OPN and were inhibited in normal VSMCs by incubation with 1 microgram/ml anti-OPN antibody. The autocrine secretion of OPN appears to play an important role in VSMC adhesion, spreading, and invasion.

This article has been cited by other articles:

				M. Scatena, L. Liaw, and C. M. Giachelli Osteopontin: A Multifunctional Molecule Regulating Chronic Inflammation and Vascular Disease Arterioscler. Thromb. Vasc. Biol., November 1, 2007; 27(11): 2302 - 2309. [Abstract] [Full Text] [PDF]

				J. Cheng, D.-H. Huo, D.-M. Kuang, J. Yang, L. Zheng, and S.-M. Zhuang Human Macrophages Promote the Motility and Invasiveness of Osteopontin-Knockdown Tumor Cells Cancer Res., June 1, 2007; 67(11): 5141 - 5147. [Abstract] [Full Text] [PDF]

				E. Geissinger, C. Weisser, P. Fischer, M. Schartl, and C. Wellbrock Autocrine Stimulation by Osteopontin Contributes to Antiapoptotic Signalling of Melanocytes in Dermal Collagen Cancer Res., August 15, 2002; 62(16): 4820 - 4828. [Abstract] [Full Text] [PDF]

				E. Cavusoglu, I. Chen, J. Rappaport, and J. D. Marmur Inhibition of Tissue Factor Gene Induction and Activity Using a Hairpin Ribozyme Circulation, May 14, 2002; 105(19): 2282 - 2287. [Abstract] [Full Text] [PDF]

				G. G. Bishop, J. A. McPherson, J. M. Sanders, S. E. Hesselbacher, M. J. Feldman, C. A. McNamara, L. W. Gimple, E. R. Powers, S. A. Mousa, and I. J. Sarembock Selective {{alpha}}v{beta}3-Receptor Blockade Reduces Macrophage Infiltration and Restenosis After Balloon Angioplasty in the Atherosclerotic Rabbit Circulation, April 10, 2001; 103(14): 1906 - 1911. [Abstract] [Full Text] [PDF]

				G. Li, Y.-F. Chen, S. S. Kelpke, S. Oparil, and J. A. Thompson Estrogen Attenuates Integrin-{beta}3-Dependent Adventitial Fibroblast Migration After Inhibition of Osteopontin Production in Vascular Smooth Muscle Cells Circulation, June 27, 2000; 101(25): 2949 - 2955. [Abstract] [Full Text] [PDF]

				M. Nemir, D. Bhattacharyya, X. Li, K. Singh, A. B. Mukherjee, and B. B. Mukherjee Targeted Inhibition of Osteopontin Expression in the Mammary Gland Causes Abnormal Morphogenesis and Lactation Deficiency J. Biol. Chem., January 14, 2000; 275(2): 969 - 976. [Abstract] [Full Text] [PDF]

				J. A. Ellison, J. J. Velier, P. Spera, Z. L. Jonak, X. Wang, F. C. Barone, G. Z. Feuerstein, T. Abumiya, and G. J. del Zoppo Osteopontin and its Integrin Receptor {alpha}vß3 Are Upregulated During Formation of the Glial Scar After Focal Stroke • Editorial Comment Stroke, August 1, 1998; 29(8): 1698 - 1707. [Abstract] [Full Text] [PDF]

				L. Liaw, D. M. Lombardi, M. M. Almeida, S. M. Schwartz, D. deBlois, and C. M. Giachelli Neutralizing Antibodies Directed Against Osteopontin Inhibit Rat Carotid Neointimal Thickening After Endothelial Denudation Arterioscler. Thromb. Vasc. Biol., January 1, 1997; 17(1): 188 - 193. [Abstract] [Full Text]

				H. Chaulet, C. Desgranges, M.-A. Renault, F. Dupuch, G. Ezan, F. Peiretti, G. Loirand, P. Pacaud, and A.-P. Gadeau Extracellular Nucleotides Induce Arterial Smooth Muscle Cell Migration Via Osteopontin Circ. Res., October 26, 2001; 89(9): 772 - 778. [Abstract] [Full Text] [PDF]