This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moller, P. Articles by Krammer, P. H. Search for Related Content PubMed PubMed Citation Articles by Moller, P. Articles by Krammer, P. H.

American Journal of Pathology, Vol 149, 9-13, Copyright © 1996 by American Society for Investigative Pathology

REGULAR ARTICLES

Paneth cells express high levels of CD95 ligand transcripts: a unique property among gastrointestinal epithelia

P Moller, H Walczak, S Reidl, J Strater and PH Krammer
Institute of Pathology of the University of Ulm, Germany.

CD95 (APO-1/Fas), a cell surface receptor and member of the tumor necrosis factor receptor superfamily, induces apoptosis upon oligomerization. CD95 is broadly expressed in normal tissues. The CD95 ligand (CD95L) is a member of the tumor necrosis factor family of cytokines and exists in a membrane-bound and in a soluble form. In vitro, CD95L is expressed and released by activated T lymphocytes. The range of cell types capable of expressing CD95L in vivo is unknown so far. Using a specific probe for human CD95L and sensitive in situ hybridization, we examined CD95L mRNA expression along the gastrointestinal tract. The scarce lymphohistiocytic infiltrate within the lamina propria contained small subsets of medium-sized labeled cells, some of which bad short cytoplasmic protrusions and others of which were lymphoid in morphology. Autochthonous cells of the gastrointestinal tract did not contain any detectable transcripts except for Paneth cells that expressed CD95L mRNA at high levels. In ulcerative colitis, CD95L mRNA-positive inflammatory cells were increased in number, and metaplastic Paneth cells were the only epithelial cells expressing CD95L. Paneth cells are CD95 negative. Therefore, these cells may not commit CD95-mediated autocrine suicide. By secreting soluble CD95L, however, the Paneth cells might contribute to mucosal integrity.

This article has been cited by other articles:

				N. L. Jones, A. S. Day, H. Jennings, P. T. Shannon, E. Galindo-Mata, and P. M. Sherman Enhanced Disease Severity in Helicobacter pylori-Infected Mice Deficient in Fas Signaling Infect. Immun., May 1, 2002; 70(5): 2591 - 2597. [Abstract] [Full Text] [PDF]

				S. Jodo, S. Xiao, A. Hohlbaum, D. Strehlow, A. Marshak-Rothstein, and S.-T. Ju Apoptosis-inducing Membrane Vesicles. A NOVEL AGENT WITH UNIQUE PROPERTIES J. Biol. Chem., October 19, 2001; 276(43): 39938 - 39944. [Abstract] [Full Text] [PDF]

				C. Zietz, U. Rumpler, M. Sturzl, and U. Lohrs Inverse Relation of Fas-Ligand and Tumor-Infiltrating Lymphocytes in Angiosarcoma : Indications of Apoptotic Tumor Counterattack Am. J. Pathol., September 1, 2001; 159(3): 963 - 970. [Abstract] [Full Text] [PDF]

				M. J. Pinkoski, T. Brunner, D. R. Green, and T. Lin Fas and Fas ligand in gut and liver Am J Physiol Gastrointest Liver Physiol, March 1, 2000; 278(3): G354 - G366. [Abstract] [Full Text] [PDF]

				X.-M. Chen, G. J. Gores, C. V. Paya, and N. F. LaRusso Cryptosporidium parvum induces apoptosis in biliary epithelia by a Fas/Fas ligand-dependent mechanism Am J Physiol Gastrointest Liver Physiol, September 1, 1999; 277(3): G599 - G608. [Abstract] [Full Text] [PDF]

				Y.-Y. Fan, J. Zhang, R. Barhoumi, R. C. Burghardt, N. D. Turner, J. R. Lupton, and R. S. Chapkin Antagonism of CD95 signaling blocks butyrate induction of apoptosis in young adult mouse colonic cells Am J Physiol Cell Physiol, August 1, 1999; 277(2): C310 - C319. [Abstract] [Full Text] [PDF]

				C. Kakinuma, K. Takagaki, T. Yatomi, N. Nakamura, S. Nagata, A. Uemura, and Y. Shibutani Acute Toxicity of an Anti-Fas Antibody in Mice Toxicol Pathol, July 1, 1999; 27(4): 412 - 420. [Abstract] [PDF]

				F. Sbih-Lammali, B. Clausse, H. Ardila-Osorio, R. Guerry, M. Talbot, S. Havouis, L. Ferradini, J. Bosq, T. Tursz, and P. Busson Control of Apoptosis in Epstein Barr Virus-positive Nasopharyngeal Carcinoma Cells: Opposite Effects of CD95 and CD40 Stimulation Cancer Res., February 1, 1999; 59(4): 924 - 930. [Abstract] [Full Text] [PDF]

				J. Strater, S. M. Mariani, H. Walczak, F. G. Rucker, F. Leithauser, P. H. Krammer, and P. Moller CD95 Ligand (CD95L) in Normal Human Lymphoid Tissues : A Subset of Plasma Cells Are Prominent Producers of CD95L Am. J. Pathol., January 1, 1999; 154(1): 193 - 201. [Abstract] [Full Text] [PDF]

				K. J. Hamann, D. R. Dorscheid, F. D. Ko, A. E. Conforti, A. I. Sperling, K. F. Rabe, and S. R. White Expression of Fas (CD95) and FasL (CD95L) in Human Airway Epithelium Am. J. Respir. Cell Mol. Biol., October 1, 1998; 19(4): 537 - 542. [Abstract] [Full Text]

				H Ueyama, T Kiyohara, N Sawada, K Isozaki, S Kitamura, S Kondo, J Miyagawa, S Kanayama, Y Shinomura, H Ishikawa, et al. High Fas ligand expression on lymphocytes in lesions of ulcerative colitis Gut, July 1, 1998; 43(1): 48 - 55. [Abstract] [Full Text] [PDF]

				B. A. Jones and G. J. Gores Physiology and pathophysiology of apoptosis in epithelial cells of the liver, pancreas, and intestine Am J Physiol Gastrointest Liver Physiol, December 1, 1997; 273(6): G1174 - G1188. [Abstract] [Full Text] [PDF]