This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yatabe, Y. Articles by Mori, N. Search for Related Content PubMed PubMed Citation Articles by Yatabe, Y. Articles by Mori, N.

American Journal of Pathology, Vol 149, 1351-1361, Copyright © 1996 by American Society for Investigative Pathology

REGULAR ARTICLES

Poor correlation between clonal immunoglobulin gene rearrangement and immunoglobulin gene transcription in Hodgkin's disease

Y Yatabe, K Oka, J Asai and N Mori
First Department of Pathology, Nagoya University, School of Medicine, Japan.

It recently has been suggested that some cases of Hodgkin's disease (HD) are derived from B lymphocytes. We therefore examined immunoglobulin heavy chain gene rearrangement and transcription by polymerase chain reaction in 30 cases of HD and compared them with 25 cases of non-Hodgkin's B-cell lymphoma. Clonal VDJ gene rearrangements were observed in 6 cases of HD. Of these 6 cases, clonal VDJ gene transcription was found by RNA-dependent polymerase chain reaction in only 2 cases. One of these 2 cases histologically showed composite lymphoma of HD and diffuse large B cell lymphoma. We performed in situ hybridization with a clonal VDJ gene probe in this case to determine the localization of the genetic clonality. The large lymphoma cells reacted positively, but Hodgkin's and Reed-Sternberg cells showed only nonspecific staining. No discrepancy between immunoglobulin gene rearrangement and transcription was observed in 24 of the 25 cases of non-Hodgkin's B-cell lymphoma. The discordance between clonal gene rearrangement and transcription in HD contrasts sharply with the high correlation of those processes in non-Hodgkin's B-cell lymphoma.

This article has been cited by other articles:

				T. Marafioti, M. Hummel, H.-D. Foss, H. Laumen, P. Korbjuhn, I. Anagnostopoulos, H. Lammert, G. Demel, J. Theil, T. Wirth, et al. Hodgkin and Reed-Sternberg cells represent an expansion of a single clone originating from a germinal center B-cell with functional immunoglobulin gene rearrangements but defective immunoglobulin transcription Blood, February 15, 2000; 95(4): 1443 - 1450. [Abstract] [Full Text] [PDF]

				A. Jox, T. Zander, R. Kuppers, J. Irsch, H. Kanzler, M. Kornacker, H. Bohlen, V. Diehl, and J. Wolf Somatic Mutations Within the Untranslated Regions of Rearranged Ig Genes in a Case of Classical Hodgkin's Disease as a Potential Cause for the Absence of Ig in the Lymphoma Cells Blood, June 1, 1999; 93(11): 3964 - 3972. [Abstract] [Full Text] [PDF]