| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 149, 1803-1811, Copyright © 1996 by American Society for Investigative Pathology
REGULAR ARTICLES |
K Ashida, H Nakatsukasa, T Higashi, S Ohguchi, N Hino, K Nouso, Y Urabe, K Yoshida, N Kinugasa and T Tsuji
First Department of Internal Medicine, Okayama University Medical School, Japan.
To examine the possible involvement of gelatinase B in human hepatocellular carcinoma (HCC), cellular localization of transcripts and protein of gelatinase B were studied by using in situ hybridization and immunohistochemistry. Transcripts for gelatinase B were observed in tumor cells in 22 cases of 27 HCCs and also in dysplastic nodules. However, there was no significant difference in the expression among histological grades of HCC. The expression was mostly homogeneous, but the intensity varied with the nodules. Of 13 cases with capsular invasion, 12 expressed gelatinase B, whereas 10 of 14 without capsular invasion did (P < 0.05). Gelatinase B transcripts were commonly observed in the sinusoidal cells of the hepatic lobules, in mesenchymal cells both in fibrous capsules and around the necrosis, and also in some undefined cells of the portal tracts of noncancerous liver. Localization of gelatinase B protein was mostly similar to but sometimes different from that of the transcripts in cancer nodules. In conclusion, the expression of gelatinase B appears to be an important characteristic of malignant transformation of hepatocytes. The findings suggest that gelatinase B synthesized by cancer cells plays an important role in the growth and invasion of HCC by degrading surrounding extracellular matrices.
This article has been cited by other articles:
 |
|
 |
|
  Y.-P. Han, L. Zhou, J. Wang, S. Xiong, W. L. Garner, S. W. French, and H. Tsukamoto Essential Role of Matrix Metalloproteinases in Interleukin-1-induced Myofibroblastic Activation of Hepatic Stellate Cell in Collagen J. Biol. Chem., February 6, 2004; 279(6): 4820 - 4828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kaneyoshi, H. Nakatsukasa, T. Higashi, K. Fujiwara, I. Naito, K. Nouso, K. Kariyama, Y. Kobayashi, M. Uemura, S.-i. Nakamura, et al. Actual Invasive Potential of Human Hepatocellular Carcinoma Revealed by in Situ Gelatin Zymography Clin. Cancer Res., December 1, 2001; 7(12): 4027 - 4032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Zucker, M. Hymowitz, E. E. Rollo, R. Mann, C. E. Conner, J. Cao, H. D. Foda, D. C. Tompkins, and B. P. Toole Tumorigenic Potential of Extracellular Matrix Metalloproteinase Inducer Am. J. Pathol., June 1, 2001; 158(6): 1921 - 1928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ozaki, T. Mizuta, G. Zhao, H. Yotsumoto, T. Hara, S. Kajihara, A. Hisatomi, T. Sakai, and K. Yamamoto Involvement of the Ets-1 Gene in Overexpression of Matrilysin in Human Hepatocellular Carcinoma Cancer Res., November 1, 2000; 60(22): 6519 - 6525. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. A. Raithatha, H. Muzik, N. B. Rewcastle, R. N. Johnston, D. R. Edwards, and P. A. Forsyth Localization of gelatinase-A and gelatinase-B mRNA and protein in human gliomas Neuro-oncol, July 1, 2000; 2(3): 145 - 150. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Lichtinghagen, O. Huegel, T. Seifert, C. I. Haberkorn, D. Michels, P. Flemming, M. Bahr, and K. H.W. Boeker Expression of Matrix Metalloproteinase-2 and -9 and Their Inhibitors in Peripheral Blood Cells of Patients with Chronic Hepatitis C Clin. Chem., February 1, 2000; 46(2): 183 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Arnold, A. B. Young, R. K. Munn, R. A. Patchell, N. Nanayakkara, and W. R. Markesbery Expression of p53, bcl-2, E-Cadherin, Matrix Metalloproteinase-9, and Tissue Inhibitor of Metalloproteinases-1 in Paired Primary Tumors and Brain Metastasis Clin. Cancer Res., December 1, 1999; 5(12): 4028 - 4033. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
