This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moriyama, H. Articles by Naito, M. Search for Related Content PubMed PubMed Citation Articles by Moriyama, H. Articles by Naito, M.

American Journal of Pathology, Vol 150, 2047-2060, Copyright © 1997 by American Society for Investigative Pathology

REGULAR ARTICLES

Expression of macrophage colony-stimulating factor and its receptor in hepatic granulomas of Kupffer-cell-depleted mice

H Moriyama, T Yamamoto, H Takatsuka, H Umezu, K Tokunaga, T Nagano, M Arakawa and M Naito
Second Department of Pathology, Niigata University School of Medicine, Japan.

In mice administered with liposome-entrapped dichloromethylene diphosphonate, which depletes Kupffer cells, the size and the number of zymosan-induced granulomas in the liver were smaller than in untreated mice. The number of macrophage precursors, as detected by the monoclonal antibodies for macrophage precursors, increased after zymosan injection in both groups of mice, proliferated, and differentiated into macrophages. Expression of macrophage colony- stimulating factor (M-CSF), interleukin-1, monocyte chemoattractant protein-1, tumor necrosis factor-alpha, and interferon-gamma mRNA was enhanced in the stage of granuloma formation in the control mouse liver, whereas it was suppressed in Kupffer-cell-depleted mice. However, M-CSF mRNA expression was increased in the Kupffer-cell- depleted mice to form granulomas in the late stages. In situ hybridization demonstrated the expression of M-CSF mRNA and c-fms mRNA in Kupffer cells and monocyte-derived macrophages in the sinusoid and granulomas. The concentration of M-CSF in serum of zymosan-injected control mice was within normal range, but that of zymosan-treated or untreated Kupffer-cell-depleted mice was markedly elevated at day 1. These findings imply that Kupffer cells are indispensable for granuloma formation and produce various cytokines including M-CSF. The local production and consumption of M-CSF in the liver may play a crucial role in granulomatous inflammation.

This article has been cited by other articles:

				H. Moriyama, M. Kobayashi, T. Takada, T. Shimizu, M. Terada, J.-I. Narita, M. Maruyama, K. Watanabe, E. Suzuki, and F. Gejyo Two-dimensional Analysis of Elements and Mononuclear Cells in Hard Metal Lung Disease Am. J. Respir. Crit. Care Med., July 1, 2007; 176(1): 70 - 77. [Abstract] [Full Text] [PDF]

				J. Yamate, Y. Machida, M. Ide, M. Kuwamura, T. Kotani, O. Sawamoto, and J. LaMarre Cisplatin-Induced Renal Interstitial Fibrosis in Neonatal Rats, Developing as Solitary Nephron Unit Lesions Toxicol Pathol, February 1, 2005; 33(2): 207 - 217. [Abstract] [Full Text] [PDF]

				J. Morimoto, M. Inobe, C. Kimura, S. Kon, H. Diao, M. Aoki, T. Miyazaki, D. T. Denhardt, S. Rittling, and T. Uede Osteopontin affects the persistence of {beta}-glucan-induced hepatic granuloma formation and tissue injury through two distinct mechanisms Int. Immunol., March 1, 2004; 16(3): 477 - 488. [Abstract] [Full Text] [PDF]

				K. Tanaka, J. Morimoto, S. Kon, C. Kimura, M. Inobe, H. Diao, G. Hirschfeld, J. M. Weiss, and T. Uede Effect of Osteopontin Alleles on {beta}-Glucan-Induced Granuloma Formation in the Mouse Liver Am. J. Pathol., February 1, 2004; 164(2): 567 - 575. [Abstract] [Full Text] [PDF]

				J. Yamate, K. Sato, M. Ide, M. Nakanishi, M. Kuwamura, S. Sakuma, and S. Nakatsuji Participation of Different Macrophage Populations and Myofibroblastic Cells in Chronically Developed Renal Interstitial Fibrosis after Cisplatin-induced Renal Injury in Rats Vet. Pathol., May 1, 2002; 39(3): 322 - 333. [Abstract] [Full Text] [PDF]

				S. Kawasaki, H. Takizawa, H. Yoneyama, T. Nakayama, R. Fujisawa, M. Izumizaki, T. Imai, O. Yoshie, I. Homma, K. Yamamoto, et al. Intervention of Thymus and Activation-Regulated Chemokine Attenuates the Development of Allergic Airway Inflammation and Hyperresponsiveness in Mice J. Immunol., February 1, 2001; 166(3): 2055 - 2062. [Abstract] [Full Text] [PDF]

				A. A. Wynn, K. Miyakawa, E. Miyata, G. Dranoff, M. Takeya, and K. Takahashi Role of Granulocyte/Macrophage Colony-Stimulating Factor in Zymocel-Induced Hepatic Granuloma Formation Am. J. Pathol., January 1, 2001; 158(1): 131 - 145. [Abstract] [Full Text] [PDF]

				A. Sugihara, Y. Adachi, M. Inaba, H. Hisha, K. Sugiura, S. Miyashima, Y. Amoh, S. Taketani, H. Oyaizu, K. Ikebukuro, et al. Age-Dependent Abnormalities of Hematopoietic Stem Cells in (NZW BXSB)F1 Mice Stem Cells, November 1, 1999; 17(6): 357 - 365. [Abstract] [Full Text]

				S.-I. Hagiwara, M. Takeya, H. Suzuki, T. Kodama, L. J. W. van der Laan, G. Kraal, N. Kitamura, and K. Takahashi Role of Macrophage Scavenger Receptors in Hepatic Granuloma Formation in Mice Am. J. Pathol., March 1, 1999; 154(3): 705 - 720. [Abstract] [Full Text] [PDF]